Publicação
Evaluation of the ability to form biofilms in KPC-producing and ESBL-producing Klebsiella pneumoniae isolated from clinical samples
| datacite.subject.fos | Ciências Médicas::Ciências da Saúde | |
| datacite.subject.sdg | 03:Saúde de Qualidade | |
| dc.contributor.author | Sabença, Carolina | |
| dc.contributor.author | Costa, Eliana | |
| dc.contributor.author | Sousa, Sara | |
| dc.contributor.author | Barros, Lillian | |
| dc.contributor.author | Oliveira, Ana | |
| dc.contributor.author | Ramos, Sónia | |
| dc.contributor.author | Igrejas, Gilberto | |
| dc.contributor.author | Torres, Carmen | |
| dc.contributor.author | Poeta, Patrícia | |
| dc.date.accessioned | 2026-02-20T17:03:15Z | |
| dc.date.available | 2026-02-20T17:03:15Z | |
| dc.date.issued | 2023-07 | |
| dc.description.abstract | The appearance of Klebsiella pneumoniae strains producing extended-spectrum β-lactamase (ESBL), and carbapenemase (KPC) has turned into a significant public health issue. ESBL- and KPC-producing K. pneumoniae’s ability to form biofilms is a significant concern as it can promote the spread of antibiotic resistance and prolong infections in healthcare facilities. A total of 45 K. pneumoniae strains were isolated from human infections. Antibiograms were performed for 17 antibiotics, ESBL production was tested by Etest ESBL PM/PML, a rapid test was used to detect KPC carbapenemases, and resistance genes were detected by PCR. Biofilm production was detected by the microtiter plate method. A total of 73% of multidrug resistance was found, with the highest resistance rates to ampicillin, trimethoprim–sulfamethoxazole, cefotaxime, amoxicillin-clavulanic acid, and aztreonam. Simultaneously, the most effective antibiotics were tetracycline and amikacin. blaCTX-M, blaTEM, blaSHV, aac(3)-II, aadA1, tetA, cmlA, catA, gyrA, gyrB, parC, sul1, sul2, sul3, blaKPC, blaOXA, and blaPER genes were detected. Biofilm production showed that 80% of K. pneumoniae strains were biofilm producers. Most ESBL- and KPC-producing isolates were weak biofilm producers (40.0% and 60.0%, respectively). There was no correlation between the ability to form stronger biofilms and the presence of ESBL and KPC enzymes in K. pneumoniae isolates. | eng |
| dc.identifier.citation | Sabença C, Costa E, Sousa S, Barros L, Oliveira A, Ramos S, Igrejas G, Torres C, Poeta P. Evaluation of the Ability to Form Biofilms in KPC-Producing and ESBL-Producing Klebsiella pneumoniae Isolated from Clinical Samples. Antibiotics. 2023; 12(7):1143. https://doi.org/10.3390/antibiotics12071143 | |
| dc.identifier.doi | 10.3390/antibiotics12071143 | |
| dc.identifier.issn | 2079-6382 | |
| dc.identifier.uri | http://hdl.handle.net/10400.26/61812 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | MDPI | |
| dc.relation.hasversion | https://doi.org/10.3390/antibiotics12071143 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Klebsiella pneumoniae | |
| dc.subject | extended-spectrum β-lactamase (ESBL) | |
| dc.subject | Klebsiella pneumoniae carbapenemase (KPC) | |
| dc.subject | biofilm | |
| dc.subject | antimicrobial resistance | |
| dc.title | Evaluation of the ability to form biofilms in KPC-producing and ESBL-producing Klebsiella pneumoniae isolated from clinical samples | eng |
| dc.type | contribution to journal | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 7 | |
| oaire.citation.startPage | 1143 | |
| oaire.citation.title | Antibiotics | |
| oaire.citation.volume | 12 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |