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Publication
Avaliação da correlação entre a presença de Salmonela SPP. e microorganismos indicadores de higiene em carcaças de suínos
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Authors
Advisor(s)
Abstract(s)
As bactérias do género Salmonella spp. são responsáveis por infeções em
suínos e em seres humanos, influenciando assim a produção de carne e também a
saúde pública.
O presente estudo teve como objetivo mapear a distribuição da contaminação
microbiológica, em particular no que respeita a indicadores de higiene e Salmonella
spp., em carcaças de suínos e avaliar e estabelecer uma correlação, no sentido de
poder usar a quantificação de aeróbios totais a 30°C e Enterobacteriaceae, como
indicadores fiáveis da presença de Salmonella spp., com o intuito de evitar/reduzir as
análises morosas e dispendiosas de pesquisa deste patógeno.
Com este propósito foram utilizados dados compreendidos entre o dia 20 de
junho de 2017 e 10 de junho de 2019. Neste período de tempo foram analisadas 525
amostras pertencentes a um matadouro no distrito de Coimbra, recolhidas no
corredor de expedição, antes da refrigeração. Em todas as amostras, foi pesquisada a
presença de Salmonella spp. e quantificados os aeróbios totais a 30°C e
Enterobacteriaceae.
Relativamente à quantificação de Enterobacteriaceae, foram determinados
valores entre 0,70 Log10 ufc/cm2
e 4,32 Log10 ufc/cm2
, com uma média de 1,72 ± 0,72
Log10 ufc/cm2
. Na quantificação de aeróbios totais, os valores variaram entre 0,90 Log10
ufc/cm2
e 5,88 Log10 ufc/cm2
, com um valor médio de 3,33 ± 0,66 Log10 ufc/cm2
.
No que concerne a Salmonella spp., do total de amostras analisadas, esta foi
detetada em apenas 42 amostras, as quais apresentaram valores de indicadores de
higiene de diversas ordens de grandeza. Desta forma, não foi possível estabelecer
estatisticamente uma correlação positiva entre a contagem de indicadores de higiene
e a presença de Salmonella spp., como se procurou inicialmente com este estudo.
Palavras-chave: Carcaças de suínos, Enterobacteriaceae, Indicadores de Higiene,
Salmonella
Bacteria of the genus Salmonella spp. are responsible for infections in pigs and humans, thus influencing meat production and also public health. The present study aimed to map the distribution of microbiological contamination, in particular concerning hygiene indicators and Salmonella spp., in pig carcasses and to evaluate and establish a correlation in order to use the total aerobic bacteria at 30 °C and Enterobactereaceae as reliable indicators of the presence of Salmonella spp., in order to avoid/reduce the time consuming and expensive analysis of this pathogen. For this purpose, data were used between June 20, 2017 and June 10, 2019. During this period, 525 samples belonging to a slaughterhouse in the district of Coimbra, collected in the expedition corridor, before refrigeration were analyzed. In all samples, the presence of Salmonella spp. was investigated and total aerobic bacteria at 30 °C and Enterobactereaceae were quantified. Regarding the quantification of Enterobactereaceae, it presented values between 0.70 Log10cfu/cm2 and 4.32 Log10cfu/cm2 , with an average of 1.72 ± 0.72 Log10cfu/cm2 , for the total aerobic quantification, the values ranged from 0.90 Log10cfu/cm2 to 5.88 Log10cfu/cm2 , with an average value of 3.33 ± 0.66 Log10cfu/cm2 . As far as Salmonella spp. is concerned, out of the total samples analyzed, it was detected in only 42 samples, which presented hygiene indicator values of several orders of magnitude. Thus, it was not possible to statistically establish a positive correlation between the hygiene indicator count and the presence of Salmonella spp., as initially sought with this study. Key words: Enterobactereaceae, Hygiene Indicators, pig carcasses, Salmonella
Bacteria of the genus Salmonella spp. are responsible for infections in pigs and humans, thus influencing meat production and also public health. The present study aimed to map the distribution of microbiological contamination, in particular concerning hygiene indicators and Salmonella spp., in pig carcasses and to evaluate and establish a correlation in order to use the total aerobic bacteria at 30 °C and Enterobactereaceae as reliable indicators of the presence of Salmonella spp., in order to avoid/reduce the time consuming and expensive analysis of this pathogen. For this purpose, data were used between June 20, 2017 and June 10, 2019. During this period, 525 samples belonging to a slaughterhouse in the district of Coimbra, collected in the expedition corridor, before refrigeration were analyzed. In all samples, the presence of Salmonella spp. was investigated and total aerobic bacteria at 30 °C and Enterobactereaceae were quantified. Regarding the quantification of Enterobactereaceae, it presented values between 0.70 Log10cfu/cm2 and 4.32 Log10cfu/cm2 , with an average of 1.72 ± 0.72 Log10cfu/cm2 , for the total aerobic quantification, the values ranged from 0.90 Log10cfu/cm2 to 5.88 Log10cfu/cm2 , with an average value of 3.33 ± 0.66 Log10cfu/cm2 . As far as Salmonella spp. is concerned, out of the total samples analyzed, it was detected in only 42 samples, which presented hygiene indicator values of several orders of magnitude. Thus, it was not possible to statistically establish a positive correlation between the hygiene indicator count and the presence of Salmonella spp., as initially sought with this study. Key words: Enterobactereaceae, Hygiene Indicators, pig carcasses, Salmonella
Description
Keywords
Carcaças de suínos Enterobacteriaceae Enterobacteriaceae Salmonella