Publicação
Enhanced efficacy of laser-activated irrigation (Er,Cr:YSGG) in eradicating Enterococcus faecalis biofilm in 3D-printed molar replicas : a pilot study
| datacite.subject.fos | Ciências Médicas::Ciências da Saúde | |
| datacite.subject.sdg | 03:Saúde de Qualidade | |
| dc.contributor.author | Macedo, Margarida | |
| dc.contributor.author | Neves, João Albernaz | |
| dc.contributor.author | Pérez, Alejandro R. | |
| dc.contributor.author | Proença, Luís | |
| dc.contributor.author | Bessa, Lucinda J. | |
| dc.date.accessioned | 2026-06-08T14:03:37Z | |
| dc.date.available | 2026-06-08T14:03:37Z | |
| dc.date.issued | 2026-04 | |
| dc.description.abstract | Objectives: This study compared the efficacy of Sonic (EDDY) and Er,Cr:YSGG (2780 nm) laser activation in eradicating Enterococcus faecalis biofilm formed in 3D-printed molar replicas with two mesial canals and one distal canal. Materials and Methods: An in vitro design was implemented using 20 3D-printed mandibular molar replicas mimicking the natural canal morphology. Root canals were inoculated with E. faecalis and incubated for 21 days to allow the development of a mature biofilm. Three irrigation protocols were tested: Conventional needle irrigation (CNI), EDDY sonic activation (SA), and Er,Cr:YSGG (2780 nm) laser activation (LA). The control group was irrigated with phosphate-buffered saline (PBS) without activation. Residual bacterial load was quantified through colony-forming unit (CFU) counts and quantitative PCR (qPCR). Bacterial viability in the apical isthmus was assessed using fluorescence microscopy. A Student's t-test was performed to identify significant differences between CFU/mL values in groups, with significance set at 5% (p < 0.05). Results: CFU counts of E. faecalis were significantly lower in the CNI, SA, and LA groups compared to the control (p < 0.05). In the SA and LA groups, bacterial counts were reduced to the lower detection limit (< log10 CFU/mL of 1.00), suggesting near-total bacterial elimination. qPCR and fluorescence microscopy corroborated these results, providing greater differentiation between the outcomes of sonic and laser activations. Conclusions: Er,Cr:YSGG (2780 nm) laser activation showed superior efficacy in endodontic disinfection by effectively eradicating E. faecalis biofilm, including in the challenging isthmus region, representing a promising method for complex root canal anatomies. | eng |
| dc.identifier.citation | Macedo, M., J. Albernaz Neves, A. R. Pérez, L. Proença, and L. J. Bessa. 2026. “ Enhanced Efficacy of Laser-Activated Irrigation (Er,Cr:YSGG) in Eradicating Enterococcus faecalis Biofilm in 3D-Printed Molar Replicas: A Pilot Study.” Clinical and Experimental Dental Research 12: 1–10. https://doi.org/10.1002/cre2.70279 | |
| dc.identifier.doi | 10.1002/cre2.70279 | |
| dc.identifier.issn | 2057-4347 | |
| dc.identifier.uri | http://hdl.handle.net/10400.26/63545 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Wiley | |
| dc.relation.hasversion | https://doi.org/10.1002/cre2.70279 | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | 3D‐printed molar replicas | |
| dc.subject | endodontic irrigation | |
| dc.subject | Enterococcus faecalis biofilm | |
| dc.subject | Er | |
| dc.subject | Cr:YSGG laser | |
| dc.subject | sonic activation | |
| dc.title | Enhanced efficacy of laser-activated irrigation (Er,Cr:YSGG) in eradicating Enterococcus faecalis biofilm in 3D-printed molar replicas : a pilot study | eng |
| dc.type | contribution to journal | |
| dspace.entity.type | Publication | |
| oaire.citation.issue | 2 | |
| oaire.citation.startPage | e70279 | |
| oaire.citation.title | Clinical and Experimental Dental Research | |
| oaire.citation.volume | 12 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |