Loading...
Publication
Análise do perfil químico e características interfaciais da dentina radicular, após tratamento com um sistema de irrigação inovador Dual Rinse® HEDP, em comparação com irrigação convencional
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 2.06 MB | Adobe PDF |
Abstract(s)
Objetivos: Analisar o perfil químico e características interfaciais da dentina radicular após irrigação com Dual Rinse® HEDP, em comparação com irrigação sequencial de NaOCl a 3 % e EDTA a 17 %.
Materiais e métodos: Foi realizado um estudo pré-clínico com dentes monorradiculares hígidos, composto por 4 grupos: CG_X (apenas água salina); CG_Pos (NaOCl e EDTA); NaOCl_HEDP (NaOCl e HEDP); HEDP_Sol (apenas HEDP). Cada dente foi instrumentado com o sistema de limas BlueShaper, até à Z4, e irrigado consoante o grupo a que foi alocado. De seguida, os dentes foram hemi-seccionadas, e cada metade originada foi alocada ou a um ensaio de microespectroscopia de Raman, ou utilizada para ser restaurada com cilindros (n=3; 2x2 mm) de resina composta flowable auto-adesiva Constic. Os espécimes cilíndricos foram posteriormente submetidos a um ensaio de microcisalhamento (1 mm/min). Metade da amostra foi submetida a ensaios antes do envelhecimento térmico, enquanto a restante metade foi submetida a ensaios após o processo de envelhecimento. Os picos de intensidade de micro-Raman foram utilizados para calcular o rácio amida I/mineral (1650 cm-1/960cm-1), permitindo determinar a quantidade relativa de conteúdo orgânico à superfície. A análise estatística contemplou ANOVA two-way Bonferroni (p>0,05).
Resultados: Para o Rᴍ/ᴍ, não foram encontradas diferenças estatisticamente significativas, do fator tipo de “tratamento experimental” e do fator “envelhecimento” (ANOVA two-way, p>0,05). Relativamente à resistência adesiva, confirmou-se que as diferentes irrigações resultaram numa diferença estatisticamente significativa (ANOVA two-way; F=7.4; p<0,01), verificando-se diferenças apenas entre o grupo de CG_POS e o grupo NaOCL_HEDP (Bonferroni, p<0,001). A média global da resistência adesiva aumentou com o efeito do envelhecimento.
Conclusão: Os resultados sugerem que o HEDP pode ser uma alternativa promissora nos protocolos de irrigação, uma vez que provoca menor desmineralização na dentina. No entanto, continuam a ser necessários mais estudos, com ênfase em perceber se o presente protocolo poderá, ou não, afetar as restaurações pós-endodônticas.
Aim: To analyse the chemical profile and interfacial characteristics of root dentin after irrigation with Dual Rinse® HEDP, in comparison to sequential irrigation with 3% NaOCl and 17% EDTA. Materials and methods: A pre-clinical study was undertaken on healthy monoradicular teeth, which were randomly assigned to one of four groups: CG_X (saline water only); CG_P (NaOCl and EDTA); HYP_HEDP (NaOCl and HEDP); HEDP_SOLO (HEDP only). Each tooth was instrumented with the BlueShaper file system, up to Z4, and irrigated according to the group allocated. Subsequently, the teeth were hemi-sectioned, wherein each half was either allocated to a Raman micro-spectroscopy test or restored with cylinders (n=3; 2x2 mm) of Constic self-adhesive flowable composite resin. Each cylinder was placed in a milled acrylic mould, coated with standardised Teflon and light cured. Subsequently, the cylindrical specimens were subjected to a micro-shear test, with a displacement rate of 1 mm per minute. One half of the sample was analysed prior to thermal ageing, while the remaining half was analysed following the completion of the ageing process. The micro-Raman intensity peaks were used to calculate the amide I/mineral ratio (1650 cm-1/960cm-1), making it possible to determine the relative amount of organic content on the surface. Statistical analysis included Bonferroni two-way ANOVA (p>0.05). Results: For Rᴍ/ᴍ, no statistically significant differences were found between the “experimental treatment” factor and the “ageing” factor (two-way ANOVA, p>0.05). Regarding adhesive strength, it was confirmed that the different irrigations resulted in a statistically significant difference (two-way ANOVA; F=7.4; p<0.01), with differences only being found between the CG_POS group and the NaOCL_HEDP group (Bonferroni, p<0.001). The overall mean adhesive strength increased with the effect of ageing. Conclusion: The results suggest that HEDP may be a promising alternative in irrigation protocols, since it causes less demineralization in dentin. However, more studies are still needed, with an emphasis on determining whether this protocol may or may not affect the outcome of post-endodontic restorations.
Aim: To analyse the chemical profile and interfacial characteristics of root dentin after irrigation with Dual Rinse® HEDP, in comparison to sequential irrigation with 3% NaOCl and 17% EDTA. Materials and methods: A pre-clinical study was undertaken on healthy monoradicular teeth, which were randomly assigned to one of four groups: CG_X (saline water only); CG_P (NaOCl and EDTA); HYP_HEDP (NaOCl and HEDP); HEDP_SOLO (HEDP only). Each tooth was instrumented with the BlueShaper file system, up to Z4, and irrigated according to the group allocated. Subsequently, the teeth were hemi-sectioned, wherein each half was either allocated to a Raman micro-spectroscopy test or restored with cylinders (n=3; 2x2 mm) of Constic self-adhesive flowable composite resin. Each cylinder was placed in a milled acrylic mould, coated with standardised Teflon and light cured. Subsequently, the cylindrical specimens were subjected to a micro-shear test, with a displacement rate of 1 mm per minute. One half of the sample was analysed prior to thermal ageing, while the remaining half was analysed following the completion of the ageing process. The micro-Raman intensity peaks were used to calculate the amide I/mineral ratio (1650 cm-1/960cm-1), making it possible to determine the relative amount of organic content on the surface. Statistical analysis included Bonferroni two-way ANOVA (p>0.05). Results: For Rᴍ/ᴍ, no statistically significant differences were found between the “experimental treatment” factor and the “ageing” factor (two-way ANOVA, p>0.05). Regarding adhesive strength, it was confirmed that the different irrigations resulted in a statistically significant difference (two-way ANOVA; F=7.4; p<0.01), with differences only being found between the CG_POS group and the NaOCL_HEDP group (Bonferroni, p<0.001). The overall mean adhesive strength increased with the effect of ageing. Conclusion: The results suggest that HEDP may be a promising alternative in irrigation protocols, since it causes less demineralization in dentin. However, more studies are still needed, with an emphasis on determining whether this protocol may or may not affect the outcome of post-endodontic restorations.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
HEDP Dentina radicular Perfil químico Resina auto-adesiva