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As metaloproteínases-8 ativa e -9 como biomarcadores na doença periodontal : estudo piloto
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Abstract(s)
Objetivo: Sendo a doença periodontal normalmente diagnosticada numa fase tardia, em que a doença já se encontra numa fase avançada com extensa destruição dos tecidos periodontais de suporte, existe uma necessidade permanente do desenvolvimento de técnicas para identificação precoce da mesma. O objetivo da presente investigação, é avaliar a utilidade das MMP-8 ativa e MMP-9 como biomarcadores da doença periodontal, através do estudo da sua presença no fluido crevicular gengival de pacientes diagnosticados com periodontite.
Metodologia: Para a realização do presente estudo foram selecionados, na Clínica Universitária Egas Moniz, 15 pacientes saudáveis e 25 pacientes diagnosticados com periodontite, cujo diagnóstico teve por base protocolos previamente estabelecidos. Em todos os participantes as amostras de fluido crevicular gengival foram recolhidas com recurso a periopapers e a sua quantificação volumétrica foi realizada no periotron. Para a quantificação da MMP-8 ativa e de MMP-9 total nas amostras foram realizados testes através do método ELISA.
Resultados: Foram analisadas 40 amostras no total, sendo 25 indivíduos saudáveis pertencentes ao grupo de controlo e 15 doentes periodontais que integraram o grupo de estudo. A concentração de aMMP-8 no FCG não mostraram diferenças significativas entre os grupos. A concentração de MMP-9 total foi mais elevada no grupo de controlo. Entre os grupos, o volume de FCG foi semelhante e, embora sem diferenças significativas, indivíduos saudáveis e doentes periodontais, ambos fumadores, apresentaram concentrações de MMP-8 e MMP-9 superiores no FCG.
Conclusão: A análise da concentração de aMMP-8 e MMP-9 total no FCG não permitiu identificar as mesmas metaloproteínases como biomarcador confiáveis da doença periodontal.
Objective: As periodontal disease is usually diagnosed at a late stage when the disease is already advanced, with extensive destruction of the supporting periodontal tissues, there is a need to develop techniques for its early identification. The aim of this study was to evaluate the usefulness of active MMP 8 and MMP 9 as biomarkers of periodontal disease by studying their presence in the gingival crevicular fluid of patients diagnosed with periodontitis Methodology: To carry out this study, 15 healthy patients and 25 patients diagnosed with periodontitis were selected from the Egas Moniz University Clinic, whose diagnosis was based on previously established protocols. Samples of gingival crevicular fluid were taken from all participants using periopapers, and volumetric quantification of GCF was carried out on the periotron The ELISA method was used to quantify active MMP 8 and total MMP 9 in the samples. Results A total of 40 samples were analysed, with 25 healthy individuals in the control group and 15 periodontal patients in the study group. The concentration of aMMP 8 in the GCF showed no significant differences between the groups. The concentration of total MMP 9 was higher in the control group. Between the groups, the volume of GCF was similar and, although there were no significant differences, healthy individuals, and periodontal patients, both smokers, had higher concentrations of MMP 8 and MMP 9 in the GCF. Conclusion Analysing the concentration of total aMMP 8 and MMP 9 in the GFCF did not allow these metalloproteinases to be identified as a reliable biomarker of periodontal disease.
Objective: As periodontal disease is usually diagnosed at a late stage when the disease is already advanced, with extensive destruction of the supporting periodontal tissues, there is a need to develop techniques for its early identification. The aim of this study was to evaluate the usefulness of active MMP 8 and MMP 9 as biomarkers of periodontal disease by studying their presence in the gingival crevicular fluid of patients diagnosed with periodontitis Methodology: To carry out this study, 15 healthy patients and 25 patients diagnosed with periodontitis were selected from the Egas Moniz University Clinic, whose diagnosis was based on previously established protocols. Samples of gingival crevicular fluid were taken from all participants using periopapers, and volumetric quantification of GCF was carried out on the periotron The ELISA method was used to quantify active MMP 8 and total MMP 9 in the samples. Results A total of 40 samples were analysed, with 25 healthy individuals in the control group and 15 periodontal patients in the study group. The concentration of aMMP 8 in the GCF showed no significant differences between the groups. The concentration of total MMP 9 was higher in the control group. Between the groups, the volume of GCF was similar and, although there were no significant differences, healthy individuals, and periodontal patients, both smokers, had higher concentrations of MMP 8 and MMP 9 in the GCF. Conclusion Analysing the concentration of total aMMP 8 and MMP 9 in the GFCF did not allow these metalloproteinases to be identified as a reliable biomarker of periodontal disease.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Biomarcador Fluido crevicular gengival Metaloproteínase-9 Periodontite