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Changes in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excess

2015-03Journal article Restricted access
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dc.contributor.authorNair, Rashmi R.
dc.contributor.authorSilveira, Célia M.
dc.contributor.authorDiniz, Mário S.
dc.contributor.authorAlmeida, Maria G.
dc.contributor.authorMoura, José J. G.
dc.contributor.authorRivas, Maria G.
dc.date.accessioned2017-05-30T15:07:23Z
dc.date.available2017-05-30T15:07:23Z
dc.date.issued2015-03
dc.description.abstractThe activity of sulfate-reducing bacteria (SRB) intensifies the problems associated to corrosion of metals and the solution entails significant economic costs. Although molybdate can be used to control the negative effects of these organisms, the mechanisms triggered in the cells exposed to Mo-excess are poorly understood. In this work, the effects of molybdate ions on the growth and morphology of the SRB Desulfovibrio alaskensis G20 (DaG20) were investigated. In addition, the cellular localization, ion uptake and regulation of protein expression were studied. We found that molybdate concentrations ranging between 50 and 150 μM produce a twofold increase in the doubling time with this effect being more significant at 200 μM molybdate (five times increase in the doubling time). It was also observed that 500 μM molybdate completely inhibits the cellular growth. On the context of protein regulation, we found that several enzymes involved in energy metabolism, cellular division and metal uptake processes were particularly influenced under the conditions tested. An overall description of some of the mechanisms involved in the DaG20 adaptation to molybdate-stress conditions is discussed.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1007/s00775-014-1224-4pt_PT
dc.identifier.issn0949-8257
dc.identifier.issn1432-1327
dc.identifier.urihttp://hdl.handle.net/10400.26/18441
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relationMolecular aspects of molybdate inhibition in sulphate reducing bacteria.
dc.relationSTRUCTURAL AND MECHANISTIC FEATURES OF CYTOCHROME CD1 NITRITE REDUCTASE
dc.relation.publisherversionhttps://link.springer.com/article/10.1007%2Fs00775-014-1224-4pt_PT
dc.subjectSulfate-reducing bacteriapt_PT
dc.subjectHydrogen cyclingpt_PT
dc.subjectMolybdenumpt_PT
dc.subjectTungstenpt_PT
dc.subjectMicrobiologically influenced corrosionpt_PT
dc.titleChanges in metabolic pathways of Desulfovibrio alaskensis G20 cells induced by molybdate excesspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMolecular aspects of molybdate inhibition in sulphate reducing bacteria.
oaire.awardTitleSTRUCTURAL AND MECHANISTIC FEATURES OF CYTOCHROME CD1 NITRITE REDUCTASE
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/EXPL%2FBBB-BEP%2F0274%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F63061%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F79566%2F2011/PT
oaire.citation.endPage322pt_PT
oaire.citation.startPage311pt_PT
oaire.citation.titleJournal of Biological Inorganic Chemistrypt_PT
oaire.citation.volume20(2)pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctPolítica de copyright do editorpt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication2db51900-f70f-4c59-8824-c4a468d35ba9
relation.isProjectOfPublication2461d9c6-d40c-4fc3-a605-c4f71308a849
relation.isProjectOfPublicationbd7e1740-39f4-4605-b0a6-5eaed8b50229
relation.isProjectOfPublication.latestForDiscovery2db51900-f70f-4c59-8824-c4a468d35ba9

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