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Associação dos polimorfismos dos genes da IL-1 e do IL-1RA com a peri-implantite : estudo piloto
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Advisor(s)
Abstract(s)
Objetivos: Avaliar a possível associação dos polimorfismos genéticos IL-1A-889, IL-1B+3954, IL-1RN (intrão 2) e o desenvolvimento de peri-implantite, através de um estudo piloto efetuado numa população de pacientes caucasianos da Clínica Dentária Egas Moniz.
Materiais e Métodos: Foram selecionados 20 pacientes, 10 com saúde peri-implantar e 10 com peri-implantite. As amostras contendo células da mucosa jugal foram armazenadas a -20ºC e posteriormente submetidas ao processo de extração de DNA. A análise genética foi realizada através da técnica de PCR (Polymerase chain reaction), RFLP (Restriction fragment length polymorphism) e através de análise eletroforética. A análise estatística foi realizada através do programa Statistical Package for the Social Sciences (SPSS).
Resultados e Discussão: Para o gene da IL-1A-889, observou-se que o alelo mutado foi observado em maior percentagem no grupo peri-implantite comparativamente ao grupo controlo (30% vs 15% respetivamente). Para o gene IL-1B+3954, observou-se também que o alelo alterado estava presente em maior percentagem no grupo doença comparativamente ao grupo controlo (35% vs 10% respetivamente). O genótipo positivo (presença de pelo menos um alelo T em ambos os polimorfismos IL-1A-889 e IL-1B+3954), foi detetado em 6 pacientes, sendo que 5 pertenciam ao grupo doença e 1 ao grupo saúde.
Para o IL-1RN observou-se a presença do alelo 1 em todos os pacientes, à exceção de um paciente pertencente ao grupo saúde, que apresentava o alelo 1 (VNTR de 412pb) e o alelo 3 (VNTR de 326 pb). Não se observou uma diferença estatisticamente significativa quanto à presença dos polimorfismos nos genes da IL-1 e do IL-1RN entre os grupos.
Conclusão: Para o polimorfismo do gene IL-1RN não se verificaram diferenças entre os grupos. Relativamente aos polimorfismos dos genes da IL-1 não se observou uma diferença estatisticamente significativa entre o grupo saúde e doença, contudo uma tendência deve ser realçada mostrando uma potencial ligação do genótipo da IL-1 e a peri-implantite.
Objective: To evaluate the possible association of genetic polymorphisms IL-1A-889, IL-1B+3954, IL-1RN (Intron 2) and the development of peri-implantitis, through a pilot study carried out in a population of Caucasian patients from the Egas Moniz Dental Clinic. Materials and Methods: 20 patients were selected, 10 with peri-implant health and 10 with peri-implantitis. Samples containing cells from the buccal mucosa were stored at -20ºC and later submitted to the DNA extraction process. Genetic analysis was performed using the PCR (Polymerase chain reaction), RFLP (Restriction fragment length polymorphism) and electrophoretic analysis. Statistical analysis was performed using the Statistical Package for Social Sciences program (SPSS). Results and Discussion: For the IL-1A-889 gene, it was observed that the mutated allele was observed in a higher percentage in the peri-implantitis group compared to the control group (30% vs 15% respectively). For the IL-1B+3954 gene, it was also observed that the altered allele was present in a higher percentage in the disease group compared to the control group (35% vs 10% respectively). The positive genotype was detected in 6 patients, 5 belonging to the disease group and 1 to the health group. For IL-1RN, the presence of allele 1 was observed in all patients, except for one patient belonging to the health group, which had allele 1 and allele 3. There was no statistically significant difference in the presence of polymorphisms in the IL-1 and IL-1RN genes between the groups. Conclusion: For the IL-1RN gene polymorphism there were no differences between groups. Regarding IL-1 gene polymorphisms, there was no statistically significant difference between the health and disease group, however a trend should be highlighted, showing a potential link between the IL-1 genotype and peri-implantitis.
Objective: To evaluate the possible association of genetic polymorphisms IL-1A-889, IL-1B+3954, IL-1RN (Intron 2) and the development of peri-implantitis, through a pilot study carried out in a population of Caucasian patients from the Egas Moniz Dental Clinic. Materials and Methods: 20 patients were selected, 10 with peri-implant health and 10 with peri-implantitis. Samples containing cells from the buccal mucosa were stored at -20ºC and later submitted to the DNA extraction process. Genetic analysis was performed using the PCR (Polymerase chain reaction), RFLP (Restriction fragment length polymorphism) and electrophoretic analysis. Statistical analysis was performed using the Statistical Package for Social Sciences program (SPSS). Results and Discussion: For the IL-1A-889 gene, it was observed that the mutated allele was observed in a higher percentage in the peri-implantitis group compared to the control group (30% vs 15% respectively). For the IL-1B+3954 gene, it was also observed that the altered allele was present in a higher percentage in the disease group compared to the control group (35% vs 10% respectively). The positive genotype was detected in 6 patients, 5 belonging to the disease group and 1 to the health group. For IL-1RN, the presence of allele 1 was observed in all patients, except for one patient belonging to the health group, which had allele 1 and allele 3. There was no statistically significant difference in the presence of polymorphisms in the IL-1 and IL-1RN genes between the groups. Conclusion: For the IL-1RN gene polymorphism there were no differences between groups. Regarding IL-1 gene polymorphisms, there was no statistically significant difference between the health and disease group, however a trend should be highlighted, showing a potential link between the IL-1 genotype and peri-implantitis.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
DNA IL-1 Peri-implantite Polimorfismos