Loading...
Publication
Formação in vitro de biofilmes de streptococcus mutans, candida albicans e derivados da saliva humana em resina composta e resina nanohíbrida : análise dos biofilmes formados e comparação
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 1.39 MB | Adobe PDF |
Authors
Advisor(s)
Abstract(s)
Introdução: Na superfície dentária, as comunidades microbianas formam um biofilme complexo, a placa dentária. A placa dentária também se forma em materiais restauradores, usados na restauração dentária. Pretendeu-se analisar quantitativa e qualitativamente a capacidade de S. mutans, C. albicans, mistura de ambas, e da microbiota da saliva em aderir e formar biofilme em duas resinas compostas utilizadas na restauração dentária, em comparação com o esmalte dentário.
Materiais e métodos: Biofilmes de S. mutans, C. albicans e mistos (S. mutans + C. albicans) foram formados in vitro (em meio BHI) durante 48 h em três superfícies diferentes: esmalte dentário – ED, resina composta microhíbrida – Z250 (3M Filtek™ Z250), e resina composta nanohíbrida – TEC (Tetric EvoCeram®). Cada biofilme formado foi analisado quantitativamente pela determinação de unidades formadoras de colónias (UFCs) e qualitativamente por microscopia de fluorescência após coloração Live/Dead.
Resultados: No biofilme mono-espécie de C. albicans, o número de UFCs contado a partir da TEC foi mais baixo e estatisticamente diferente (p < 0.05) do contado a partir do ED e da Z250. O biofilme de S. mutans e misto (C. albicans + S. mutans) formados em cada uma das superfícies foram semelhantes. Os biofilmes formados a partir da saliva de três indivíduos com baixo risco de cárie e de três indivíduos com alto/moderado risco não apresentaram diferenças significativas. Contudo, devido ao baixo número de indivíduos e ausência de ensaios replicados não foi possível chegar a um resultado com relevância clínica.
Conclusão: De um modo geral, as superfícies Z250 e TEC proporcionaram a formação de um biofilme semelhante àquele formado no ED. Apenas o biofilme de C. albicans na TEC se apresentou menos viável. Os resultados obtidos a partir da saliva de indivíduos com baixo e com moderado/alto risco de cárie foram preliminares não sendo suficientes para chegar a conclusões definitivas.
Introduction: On the tooth surface, the microbial communities form a complex biofilm, the dental plaque. The dental plaque is also formed in restorative materials used in dental restoration. We wanted to analyze quantitatively and qualitatively the capability of S. mutans, C. albicans, a mixture of both and saliva derived microbiota of adhering and forming biofilms on two resin composites, in comparison to tooth enamel. Materials and methods: C. albicans, S. mutans and mixed biofilms (S. mutans + C. albicans) were formed in vitro (in BHI broth) for 48 h on three distinct surfaces: tooth enamel – ED, microhybrid composite resin – Z250 (3M Filtek™ Z250), and nanohybrid composite resin – TEC (Tetric EvoCeram®). Each biofilm formed was analyzed quantitatively through the determination of colony forming units (CFUs) and qualitatively through fluorescence microscopy after Live/Dead staining. Results: On the mono-species C. albicans biofilm, the number of CFUs counted on TEC was lower and statistically different (p < 0.05) than the ones counted on ED and Z250. The S. mutans biofilm and the mixed biofilm (C. albicans + S. mutans) formed on the three surfaces were considerably similar. The biofilms formed from the saliva of three individuals with low risk and three with high risk of dental caries did not present significant differences. However, due to the low number of patients in each risk group, and the fact that only one experiment was done with the saliva samples, it was not possible to reach a conclusion with clinical meaning. Conclusion: Generally, the surfaces TEC and Z250 allowed the formation of single- and dualspecies biofilms that were similar to that formed on ED. Only C. albicans biofilm formed on TEC was less viable. The results obtained from the saliva of individuals with low and high caries risk were preliminary, and not enough to reach definite conclusions.
Introduction: On the tooth surface, the microbial communities form a complex biofilm, the dental plaque. The dental plaque is also formed in restorative materials used in dental restoration. We wanted to analyze quantitatively and qualitatively the capability of S. mutans, C. albicans, a mixture of both and saliva derived microbiota of adhering and forming biofilms on two resin composites, in comparison to tooth enamel. Materials and methods: C. albicans, S. mutans and mixed biofilms (S. mutans + C. albicans) were formed in vitro (in BHI broth) for 48 h on three distinct surfaces: tooth enamel – ED, microhybrid composite resin – Z250 (3M Filtek™ Z250), and nanohybrid composite resin – TEC (Tetric EvoCeram®). Each biofilm formed was analyzed quantitatively through the determination of colony forming units (CFUs) and qualitatively through fluorescence microscopy after Live/Dead staining. Results: On the mono-species C. albicans biofilm, the number of CFUs counted on TEC was lower and statistically different (p < 0.05) than the ones counted on ED and Z250. The S. mutans biofilm and the mixed biofilm (C. albicans + S. mutans) formed on the three surfaces were considerably similar. The biofilms formed from the saliva of three individuals with low risk and three with high risk of dental caries did not present significant differences. However, due to the low number of patients in each risk group, and the fact that only one experiment was done with the saliva samples, it was not possible to reach a conclusion with clinical meaning. Conclusion: Generally, the surfaces TEC and Z250 allowed the formation of single- and dualspecies biofilms that were similar to that formed on ED. Only C. albicans biofilm formed on TEC was less viable. The results obtained from the saliva of individuals with low and high caries risk were preliminary, and not enough to reach definite conclusions.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Biofilmes orais Candida albicans Resinas compostas Streptococcus mutans