Publication
Antagonism of BST-2/Tetherin is a conserved function of the Env glycoprotein of primary HIV-2 isolates
| dc.contributor.author | Chen, Chia-Yen | |
| dc.contributor.author | Shingai, Masashi | |
| dc.contributor.author | Welbourn, Sarah | |
| dc.contributor.author | Martin, Malcolm A. | |
| dc.contributor.author | Borrego, Pedro | |
| dc.contributor.author | Taveira, Nuno | |
| dc.contributor.author | Strebel, Klaus | |
| dc.date.accessioned | 2019-12-12T12:31:03Z | |
| dc.date.available | 2019-12-12T12:31:03Z | |
| dc.date.issued | 2016-12 | |
| dc.description.abstract | Although HIV-2 does not encode a vpu gene, the ability to antagonize bone marrow stromal antigen 2 (BST-2) is conserved in some HIV-2 isolates, where it is controlled by the Env glycoprotein. We previously reported that a single-amino-acid difference between the laboratory-adapted ROD10 and ROD14 Envs controlled the enhancement of virus release (referred to here as Vpu-like) activity. Here, we investigated how conserved the Vpu-like activity is in primary HIV-2 isolates. We found that half of the 34 tested primary HIV-2 Env isolates obtained from 7 different patients enhanced virus release. Interestingly, most HIV-2 patients harbored a mixed population of viruses containing or lacking Vpu-like activity. Vpu-like activity and Envelope functionality varied significantly among Env isolates; however, there was no direct correlation between these two functions, suggesting they evolved independently. In comparing the Env sequences from one HIV-2 patient, we found that similar to the ROD10/ROD14 Envs, a single-amino-acid change (T568I) in the ectodomain of the TM subunit was sufficient to confer Vpu-like activity to an inactive Env variant. Surprisingly, however, absence of Vpu-like activity was not correlated with absence of BST-2 interaction. Taken together, our data suggest that maintaining the ability to antagonize BST-2 is of functional relevance not only to HIV-1 but also to HIV-2 as well. Our data show that as with Vpu, binding of HIV-2 Env to BST-2 is important but not sufficient for antagonism. Finally, as observed previously, the Vpu-like activity in HIV-2 Env can be controlled by single-residue changes in the TM subunit. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Chen C-Y, Shingai M, Welbourn S, Martin MA, Borrego P, Taveira N, Strebel K. 2016. Antagonism of BST-2/tetherin is a conserved function of the Env glycoprotein of primary HIV-2 isolates. J Virol 90:11062–11074. doi:10.1128/JVI.01451-16 | pt_PT |
| dc.identifier.doi | 10.1128/JVI.01451-16 | pt_PT |
| dc.identifier.issn | 0022-538X | |
| dc.identifier.issn | 1098-5514 | |
| dc.identifier.uri | http://hdl.handle.net/10400.26/30460 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | American Society for Microbiology | pt_PT |
| dc.relation.publisherversion | https://doi.org/10.1128/JVI.01451-16 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | BST-2/Tetherin | pt_PT |
| dc.subject | Env Glycoprotein | pt_PT |
| dc.subject | Primary HIV-2 Isolates | pt_PT |
| dc.title | Antagonism of BST-2/Tetherin is a conserved function of the Env glycoprotein of primary HIV-2 isolates | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 11074 | pt_PT |
| oaire.citation.startPage | 11062 | pt_PT |
| oaire.citation.title | Journal of Virology | pt_PT |
| oaire.citation.volume | 90(24) | pt_PT |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |