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Exploring the anti-aging potential of lavandula pedunculata extracts: effect on cardiac cellular senescence
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Abstract(s)
O envelhecimento é um declínio progressivo e irreversível das funções fisiológicas do organismo, levando ao comprometimento da saúde e ao aumento do risco de desenvolvimento de doenças ao longo da vida. O envelhecimento pode ser identificado através de diversos marcadores moleculares, celulares e sistémicos. Neste trabalho destaca-se a senescência celular devido ao seu papel central no envelhecimento, tornando-a um fator de risco fundamental para as doenças cardiovasculares.
A senescência celular é um mecanismo fisiológico no qual uma célula interrompe o seu ciclo celular e entra num estado de repouso permanente, permanecendo metabolicamente ativa. Durante a senescência celular ocorre um aumento de múltiplos marcadores, nomeadamente os níveis proteicos de p53/21, a atividade de SA-β-gal e a secreção de SASP. Ao longo dos anos, tem-se verificado um interesse crescente em terapias que modulam estas vias de senescência, em particular alternativas naturais capazes de retardar doenças relacionadas com a idade.
As plantas aromáticas e medicinais apresentam um elevado potencial anti-envelhecimento, pois são ricas em compostos bioativos que apresentam efeitos antioxidantes, anti-inflamatórios e cardioprotetores, podendo ajudar na prevenção e tratamento de doenças cardiovasculares. Os efeitos benéficos relatados para estas plantas podem ser atribuídos aos seus extratos, como os óleos essenciais e os extratos não voláteis ricos em compostos fenólicos. O presente estudo centra-se na Lavandula pedunculata (Mill.) Cav. e o potencial anti-senescente dos seus extratos. Estudos anteriores reportaram propriedades antioxidantes, anti- inflamatórias e antifúngica significativas para esta espécie, o que justifica investimentos adicionais, particularmente na exploração do seu potencial em estratégias antienvelhecimento. O objetivo deste trabalho foi avaliar o efeito do óleo essencial e do extrato fenólico de Lavandula pedunculata na atividade da SA-β-gal, danos na dupla hélice de ADN, área e deformações nucleares e inibidores do ciclo celular. O potencial anti-senescente foi avaliado em cardiomioblastos H9c2 com senescênciainduzida com doxorrubicina. No geral, o óleo essencial de L. pedunculata foi capaz de diminuir significativamente a atividade de SA-β-gal e yH2AX, bem como a área e as deformações nucleares e os níveis das proteínas p53/p21, em concentrações não tóxicas. Estas descobertas destacam o potencial promissor desta espécie na mitigação da senescência cardíaca. No entanto, embora os resultados sejam bastante promissores, são necessários estudos adicionais para elucidar completamente o potencial bioativo destes extratos. No geral, o presente estudo aumenta a valorização de L. pedunculata, reforçando a sua relevância industrial e potencial como agente no antienvelhecimento e promotor da saúde cardiovascular.
Aging is an irreversible and progressive decline of the body's physiological functions, leading to health impairment and increased risk of disease development throughout life. Aging can be identified through several molecular, cellular and systemic markers. Here we highlight cellular senescence due to its central role in aging, making it a key risk factor for cardiovascular diseases. Cellular senescence is a physiological mechanism in which a cell interrupts its cell cycle and enters a state of permanent rest, but remains metabolically active. During cellular senescence, there is an increase in multiple markers, namely p53/p21 protein levels, SA-β-gal activity and SASP secretion. Over the years, there has been a growing interest in therapies that modulate these senescence pathways, in particular natural alternatives capable of delaying age-related diseases. Aromatic and medicinal plants have great anti-aging potential, as they are rich in bioactive compounds that have shown antioxidant, anti-inflammatory and cardioprotective effects, which may help prevent and treat conditions such as cardiovascular diseases. The beneficial effects reported for these plants can be attributed to their extracts, such as essential oils and non-volatile extracts rich in phenolic compounds. The present study focuses on Lavandula pedunculata (Mill.) Cav. and the anti-senescence potential of its extracts. Previous studies have reported significant antioxidant, anti-inflammatory, and antifungal properties for this species, which justify further investment, particularly in exploring its potential for anti-aging strategies. The aim of this work was to evaluate the effect of the essential oil and phenolic extract of Lavandula pedunculata on SA-β-gal activity, double strand DNA damage, nuclear area and deformations, and cell cycle inhibitors. The anti- senescence potential was assessed using H9c2 cardiomyoblasts and senescence induced resorting to doxorubicin. Overall, L. pedunculata essential oil was able to significantly decrease SA-β-gal activity, yH2AX, nuclear area and deformations as well as p53/p21 protein levels, at non-toxic concentrations. These findings highlight its promising potential in mitigating cardiac senescence. Nevertheless, while the results are quite promising, further research is needed to fully elucidate the bioactive potential of these extracts. Overall, the present study enhances the valorisation of L. pedunculata, reinforcing its industrial relevance and potential as an anti-aging agent and a promoter of cardiovascular health.
Aging is an irreversible and progressive decline of the body's physiological functions, leading to health impairment and increased risk of disease development throughout life. Aging can be identified through several molecular, cellular and systemic markers. Here we highlight cellular senescence due to its central role in aging, making it a key risk factor for cardiovascular diseases. Cellular senescence is a physiological mechanism in which a cell interrupts its cell cycle and enters a state of permanent rest, but remains metabolically active. During cellular senescence, there is an increase in multiple markers, namely p53/p21 protein levels, SA-β-gal activity and SASP secretion. Over the years, there has been a growing interest in therapies that modulate these senescence pathways, in particular natural alternatives capable of delaying age-related diseases. Aromatic and medicinal plants have great anti-aging potential, as they are rich in bioactive compounds that have shown antioxidant, anti-inflammatory and cardioprotective effects, which may help prevent and treat conditions such as cardiovascular diseases. The beneficial effects reported for these plants can be attributed to their extracts, such as essential oils and non-volatile extracts rich in phenolic compounds. The present study focuses on Lavandula pedunculata (Mill.) Cav. and the anti-senescence potential of its extracts. Previous studies have reported significant antioxidant, anti-inflammatory, and antifungal properties for this species, which justify further investment, particularly in exploring its potential for anti-aging strategies. The aim of this work was to evaluate the effect of the essential oil and phenolic extract of Lavandula pedunculata on SA-β-gal activity, double strand DNA damage, nuclear area and deformations, and cell cycle inhibitors. The anti- senescence potential was assessed using H9c2 cardiomyoblasts and senescence induced resorting to doxorubicin. Overall, L. pedunculata essential oil was able to significantly decrease SA-β-gal activity, yH2AX, nuclear area and deformations as well as p53/p21 protein levels, at non-toxic concentrations. These findings highlight its promising potential in mitigating cardiac senescence. Nevertheless, while the results are quite promising, further research is needed to fully elucidate the bioactive potential of these extracts. Overall, the present study enhances the valorisation of L. pedunculata, reinforcing its industrial relevance and potential as an anti-aging agent and a promoter of cardiovascular health.
Description
Keywords
rosmaninho óleo essencial cardiovascular senolítico. lavender essential oil senolytic.