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As metaloproteinases MMP-8 e MMP-9 no fluído crevicular de indivíduos com doença periodontal
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Abstract(s)
A periodontite é uma doença inflamatória crónica e multifatorial, frequentemente diagnosticada tardiamente, quando a destruição dos tecidos de suporte já se iniciou. A
progressão da doença pode estar associada a polimorfismos em genes que codificam de proteínas envolvidas na degradação do ligamento periodontal como as metalopeptidases da matriz (MMP). Os polimorfismos de nucleotídeo único (SNP), particularmente nas regiões promotoras, podem influenciar a expressão genética e nos níveis de proteína. Vários estudos examinaram a correlação entre SNP nos promotores de MMP-8 e MMP-9, juntamente com os níveis da enzima MMP-8, e o risco de desenvolver periodontite. No entanto, os resultados permanecem inconclusivos.
Este estudo teve como objetivo investigar a associação entre os polimorfismos MMP-8 -799C>T, MMP-8 +17C>G e MMP-9 -1562C>T e os níveis das enzimas MMP-8 no fluído
gengival crevicular (FGC) com a doença periodontal, avaliando o seu potencial como biomarcadores para o diagnóstico precoce da periodontite. A amostra do estudo foi composta por 62 indivíduos, dos quais 36 com diagnóstico de periodontite e 26 com tecido periodontal saudável. As amostras de FGC foram recolhidas com tiras de papel, sendo as concentrações enzimáticas medidas por ELISA e a atividade por zimografia. A análise genética foi realizada através de PCR e RFLP, seguida de eletroforese. Foram utilizados modelos lineares generalizados (distribuição gama, ligação log) para analisar os dados com distribuição não normal.
Os resultados do estudo não demonstraram uma associação estatisticamente significativa entre os polimorfismos estudados ou os níveis de MMP-8 com a presença de
periodontite quando ajustados à idade. Apenas a idade demonstrou uma correlação negativa com os níveis de MMP-8 e a presença de periodontite. Apesar da reduzida dimensão da amostra os dados corroboram a evidência que questiona a eficácia da MMP-8 como biomarcador diagnóstico isolado para a periodontite sugerindo a necessidade de incluir outras MMP em futuras investigações.
Periodontitis is a chronic, multifactorial inflammatory disease that is frequently diagnosed at a late stage, when the destruction of supporting tissues has already begun. Its progression may be related to polymorphisms in genes encoding proteins involved in the degradation of the periodontal ligament, such as matrix metallopeptidases (MMPs). Single nucleotide polymorphisms (SNPs), eparticularly those in promoter regions, have been demonstrated to influence on gene expression and protein levels. A number of studies have examined the correlation between SNPs in the promoters of MMP-8 and MMP-9, along with the levels of the MMP-8 enzyme, and the risk of developing periodontitis. However, the results remain inconclusive. This study aimed to investigate the association between the MMP-8 polymorphisms (- 799C>T, +17C>G) and MMP-9 (-1562C>T) and the levels of the MMP-8 enzyme in gingival crevicular fluid (FGC) with periodontal disease, evaluating their potential as biomarkers for the early diagnosis of periodontitis. The study population comprised 62 individualswith 36 diagnosed withperiodontitis and 26 exhibiting healthy periodontal tissues. The colectuion of FGC sampleswas accomplished using paper strips, enzyme concentrations were measured by ELISA, and enzyme activity was assessed by zymography. Genetic analysis was performed using PCR and RFLP, followed by electrophoresis. Generalised linear models (gamma distribution, log link) were used to analyse data with a non-normal distribution. The results demonstrated an absence of a significant association between the studied polymorphisms or MMP-8 levels and the presence of periodontitis. Only age was associated with lower MMP-8 levels or the presence of periodontitis. Despite the limited sample size, the data substantiate the existing evidence questioning the efficacy of MMP-8 as a standalone diagnostic biomarker for periodontitis, underscoring the need to include other MMPs in subsequent investigations.
Periodontitis is a chronic, multifactorial inflammatory disease that is frequently diagnosed at a late stage, when the destruction of supporting tissues has already begun. Its progression may be related to polymorphisms in genes encoding proteins involved in the degradation of the periodontal ligament, such as matrix metallopeptidases (MMPs). Single nucleotide polymorphisms (SNPs), eparticularly those in promoter regions, have been demonstrated to influence on gene expression and protein levels. A number of studies have examined the correlation between SNPs in the promoters of MMP-8 and MMP-9, along with the levels of the MMP-8 enzyme, and the risk of developing periodontitis. However, the results remain inconclusive. This study aimed to investigate the association between the MMP-8 polymorphisms (- 799C>T, +17C>G) and MMP-9 (-1562C>T) and the levels of the MMP-8 enzyme in gingival crevicular fluid (FGC) with periodontal disease, evaluating their potential as biomarkers for the early diagnosis of periodontitis. The study population comprised 62 individualswith 36 diagnosed withperiodontitis and 26 exhibiting healthy periodontal tissues. The colectuion of FGC sampleswas accomplished using paper strips, enzyme concentrations were measured by ELISA, and enzyme activity was assessed by zymography. Genetic analysis was performed using PCR and RFLP, followed by electrophoresis. Generalised linear models (gamma distribution, log link) were used to analyse data with a non-normal distribution. The results demonstrated an absence of a significant association between the studied polymorphisms or MMP-8 levels and the presence of periodontitis. Only age was associated with lower MMP-8 levels or the presence of periodontitis. Despite the limited sample size, the data substantiate the existing evidence questioning the efficacy of MMP-8 as a standalone diagnostic biomarker for periodontitis, underscoring the need to include other MMPs in subsequent investigations.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Biomarcador Fluído crevicular gengival Metalo Periodontite