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Optimization and validation of an analytical method for the determination of opiates in urine using microextraction by packed sorbent

2023-12Conference object Open access
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dc.contributor.authorSimão, Ana Y.
dc.contributor.authorMonteiro, Catarina
dc.contributor.authorMarques, Hernâni
dc.contributor.authorRosado, Tiago
dc.contributor.authorBarroso, Mário
dc.contributor.authorAndraus, Maristela
dc.contributor.authorGallardo, Eugenia
dc.date.accessioned2024-01-24T12:36:26Z
dc.date.available2024-01-24T12:36:26Z
dc.date.issued2023-12
dc.descriptionComunicação apresentada no 13º Encontro Nacional de Cromatografia, 2023pt_PT
dc.description.abstractAccording to the European Monitoring Centre for Drugs and Drug Addiction (EMCDDA), approximately 1.3 million individuals have used opiates, both for medical and illicit purposes1, presenting a significant public health challenge2,3. To address this issue, methods for quantifying these substances are needed. Urine is a commonly employed matrix in clinical and forensic toxicological analyses due to its ease of collection and ample availability. Its short detection window is particularly effective for monitoring recent drug exposure4,5. This study aimed to optimize a method for determining tramadol, codeine, morphine, 6-acetylmorphine, 6- acetylcodeine, and fentanyl in urine samples (250 μL). The process involved centrifugation, acid hydrolysis, and extraction using microextraction by packed sorbent (MEPS). MEPS offered a rapid, environmentally friendly, and reusable extraction technique6. All parameters that influence the extraction were previously optimized. The method was validated following international guidelines, demonstrating excellent linearity [1 to 1000 ng/mL for all compounds, except for fentanyl (10 to 1000 ng/mL), with coefficients of determination of at least 0.99], and presenting coefficients of variation and bias ≤ 15% for precision and accuracy, except for the lowest calibrator (≤ 20%). Recoveries obtained ranged from 17 to 107%, with lowest percentages for morphine (12 to 17%). Despite the low extraction efficiency obtained for morphine, it was possible to detect concentrations as low as 1 ng/mL for all compounds, except for fentanyl (10 ng/mL). The method was successfully applied to real samples from consumers of these substances. This is the first method to use MEPS and GC-MS/MS for the simultaneous determination of these six opioids in urine samples.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/49321
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationBID/UBI-Santander Universidades/2019pt_PT
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.relationHair as a monitoring tool for new drugs: synthetic cannabinoids and opioids
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectOpiatespt_PT
dc.subjectUrinept_PT
dc.subjectMEPSpt_PT
dc.titleOptimization and validation of an analytical method for the determination of opiates in urine using microextraction by packed sorbentpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHair as a monitoring tool for new drugs: synthetic cannabinoids and opioids
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/PDQI_CENTRO/2020.09070.BD/PT
oaire.citation.conferencePlaceLisboapt_PT
oaire.citation.title13º Encontro Nacional de Cromatografiapt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPDQI_CENTRO
person.familyNameBarroso
person.givenNameMário
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.orcid0000-0001-8848-2734
person.identifier.scopus-author-id35329243100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublication.latestForDiscovery294b555e-48c4-4013-b981-b8917400c56c
relation.isProjectOfPublication4ca3c3c9-1802-4c7f-8730-9547236896fe
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