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Documenting human exposure to cannabinoids using oral fluid

2023-12Conference object Open access
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dc.contributor.authorAntunes, Mónica
dc.contributor.authorSimões, Susana
dc.contributor.authorFonseca, Suzana
dc.contributor.authorFranco, João Miguel
dc.contributor.authorGallardo, Eugenia
dc.contributor.authorBarroso, Mário
dc.date.accessioned2024-01-24T12:18:52Z
dc.date.available2024-01-24T12:18:52Z
dc.date.issued2023-12
dc.descriptionComunicação apresentada no 13º Encontro Nacional de Cromatografia, Lisboa, 17-19 dezembro 2023.pt_PT
dc.description.abstractThe importance of studying non-conventional biological matrices such as oral fluid (OF) is increasingly being recognized. This sample presents several advantages, mainly related to its collection procedure: it is non-invasive, easy to perform by non-medical personnel, can be performed under supervision to prevent adulteration, and provides low biohazard risk. OF samples are more likely to contain parent drugs, reflecting recent drug use – a major advantage of this matrix1. A fast and robust analytical methodology was developed in OF samples for the determination of tetrahydrocannabinol (THC), 11-hydroxy-tetrahydrocannabinol (THC-OH), 11-carboxytetrahydrocannabinol (THC-COOH), cannabinol (CBN) and cannabidiol (CBD) by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), aiming at documenting cannabis consumption. Briefly, 200-μL aliquots of OF were subjected to protein precipitation with a refrigerated methanol/acetonitrile mixture (80:20, v/v). After centrifugation, the extracts were evaporated to dryness, reconstituted in methanol, and 5-μL aliquots were injected into the UPLC-QTRAP-MS 6500+ (SCIEX®) system (in a 14-minute run). The analysis was carried out in MRM mode with two transitions for each compound and one transition for each internal standard. The method was validated according to the guidelines of ANSI/ASB 0362. Parameters such as ion suppression/enhancement, interferents, linearity, precision and accuracy, limits of detection and quantification, dilution integrity and stability were studied and showcased satisfactory results. The 2 ng/mL cut-off for THC3 was achieved, and the method was successfully applied to real samples (57.95- 898.28 ng/mL for THC; 0.17-4.09 ng/mL for THC-COOH; 1.26-44.57 ng/mL for CBN; 0.42-1007.86 ng/mL).pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/49320
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationCannabis Consumption in the University Population in Portugal: Determination of Biomarkers in Hair and Oral Fluid Samples Consumo de Cannabis na População Universitária em Portugal: Determinação de Biomarcadores em Amostras de Cabelo e Saliva
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCannabinoidspt_PT
dc.subjectOral Fluidpt_PT
dc.titleDocumenting human exposure to cannabinoids using oral fluidpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleCannabis Consumption in the University Population in Portugal: Determination of Biomarkers in Hair and Oral Fluid Samples Consumo de Cannabis na População Universitária em Portugal: Determinação de Biomarcadores em Amostras de Cabelo e Saliva
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/2020.05765.BD/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.citation.conferencePlaceLisboapt_PT
oaire.citation.title13º Encontro Nacional de Cromatografia, Lisboa, 17-19 dezembro, 2023.pt_PT
oaire.fundingStreamPOR_CENTRO
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSimões
person.familyNameFonseca
person.familyNameFranco
person.familyNameBarroso
person.givenNameSusana
person.givenNameSuzana
person.givenNameJoão
person.givenNameMário
person.identifier.ciencia-idC31D-C5F1-D6BD
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.orcid0000-0002-9457-1546
person.identifier.orcid0000-0001-9122-1017
person.identifier.orcid0000-0002-3268-3841
person.identifier.orcid0000-0001-8848-2734
person.identifier.scopus-author-id35329243100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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