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Publication
Avaliação do potencial citotóxico de adesivos em células de fibroblastos de rato (linha celular 3T3/SIGMA)
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Authors
Advisor(s)
Abstract(s)
Objetivo: Avaliar o potencial citotóxico de dois tipos de adesivos no mercado:
Scotchbond Universal e Optibond Solo Plus, e um adesivo em fase experimental: T1,
quando em contacto com fibroblastos de rato da linha celular 3T3 e em osteoblastos
humanos da linha celular MG63.
Métodos: Uma quantidade definida de células foi colocada em placas de 96 poços. Os
adesivos foram pincelados em caixas de petri e polimerizados de acordo com as
indicações do fabricante. Seguidamente foram imersos em meio de cultura e incubados
por 24 horas. As células foram expostas a diferentes diluições dos extratos, filtrados e não filtrados para os fibroblastos 3T3, e apenas não filtrados para os osteoblastos MG63. A viabilidade celular foi avaliada utilizando o método de MTT. A análise estatística foi feita no programa SPSS, por meio de análise de variância (ANOVA) e testes de
multicomparação de Tukey HSD. O nível de significância foi estabelecido a p < 0,05.
Resultados: Para os fibroblastos 3T3 a ordem de citotoxicidade dos extratos dos
adesivos, não filtrados foi T1 < Optibond Solo Plus < Scotchbond Universal, revelando
alterações quando filtrados: T1 < Scotchbond Universal < Optibond Solo Plus; Para os
osteoblastos MG63 o mais tóxico foi o Scotchbond Universal e o menos tóxico o T1. Conclusões: Apesar das limitações deste estudo in vitro, podemos concluir que os três
adesivos apresentam um efeito dose-dependente, maior concentração de extrato, maior o seu poder citotóxico. A filtração de um mesmo extrato, ou a sua ausência, tem efeitos na viabilidade celular, assim como a alteração do tipo de célula, sendo as MG63 mais
sensíveis. Apesar dos resultados promissores do adesivo T1, são necessários mais testes que corroborem com este estudo e avaliem outros parâmetros relativos à sua eficácia enquanto adesivo.
Objective: Evaluate the cytotoxic potential of two types of adhesives on the market: Scotchbond Universal and Optibond Solo Plus, and an adhesive in experimental phase: T1, when in contact with mouse fibroblasts of the 3T3 cell line and in human osteoblasts of the MG63 cell line. Methods: A defined number of cells was placed in 96 well plates. The adhesives were brushed in petri dishes and polymerized according to the manufacturer's indications. Then they were immersed in culture medium and incubated for 24 hours. The cells were exposed to different dilutions of extracts, filtered and unfiltered for 3T3 fibroblasts, and only unfiltered for MG63 osteoblasts. Cell viability was evaluated using the MTT method. Statistical analysis was performed in the SPSS program, through analysis of variance (ANOVA) and Tukey HSD multicomparison tests. The significance level was established at p < 0.05. Results: For 3T3 fibroblasts the cytotoxicity order of the unfiltered adhesive extracts was T1 < Optibond Solo Plus < Scotchbond Universal, revealing changes when filtered: T1 < Scotchbond Universal < Optibond Solo Plus; For MG63 osteoblasts the most toxic was Scotchbond Universal and the least toxic was T1. Conclusions: Despite the limitations of this in vitro study, we can conclude that the three adhesives present a dose-dependent effect, higher concentration of extract, higher cytotoxic power. The filtration of the same extract, or its absence, has effects on cell viability, as well as the alteration of the type of cell, being MG63 more sensitive. Despite the promising results of T1 adhesive, more tests are needed to corroborate this study and evaluate other parameters related to its effectiveness as an adhesive.
Objective: Evaluate the cytotoxic potential of two types of adhesives on the market: Scotchbond Universal and Optibond Solo Plus, and an adhesive in experimental phase: T1, when in contact with mouse fibroblasts of the 3T3 cell line and in human osteoblasts of the MG63 cell line. Methods: A defined number of cells was placed in 96 well plates. The adhesives were brushed in petri dishes and polymerized according to the manufacturer's indications. Then they were immersed in culture medium and incubated for 24 hours. The cells were exposed to different dilutions of extracts, filtered and unfiltered for 3T3 fibroblasts, and only unfiltered for MG63 osteoblasts. Cell viability was evaluated using the MTT method. Statistical analysis was performed in the SPSS program, through analysis of variance (ANOVA) and Tukey HSD multicomparison tests. The significance level was established at p < 0.05. Results: For 3T3 fibroblasts the cytotoxicity order of the unfiltered adhesive extracts was T1 < Optibond Solo Plus < Scotchbond Universal, revealing changes when filtered: T1 < Scotchbond Universal < Optibond Solo Plus; For MG63 osteoblasts the most toxic was Scotchbond Universal and the least toxic was T1. Conclusions: Despite the limitations of this in vitro study, we can conclude that the three adhesives present a dose-dependent effect, higher concentration of extract, higher cytotoxic power. The filtration of the same extract, or its absence, has effects on cell viability, as well as the alteration of the type of cell, being MG63 more sensitive. Despite the promising results of T1 adhesive, more tests are needed to corroborate this study and evaluate other parameters related to its effectiveness as an adhesive.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Sistemas adesivos Citotoxicidade Ensaio de MTT Viabilidade celular