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Estudo da camada híbrida formada com adesivos universais sobre dentina preparada com plasma não térmico atmosférico
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Objetivo: Estudar a ultramorfologia da camada híbrida formada entre dois sistemas adesivos universais e a dentina, com e sem aplicação prévia de plasma não térmico atmosférico (PNTA) de árgon, antes do sistema adesivo.
Materiais e Métodos: Foram utilizados 8 molares humanos íntegros do banco de dentes da Egas Moniz School of Health and Science. De cada dente obteve-se uma fatia de dentina média com micrótomo de tecidos duros (Accutom-50, Struers, Ballerup, Dinamarca), posteriormente dividida em duas metades para a realização do procedimento restaurador com mesmo sistema adesivo, mas diferindo na prévia ou não aplicação de PNTA. Os sistemas adesivos estudados foram Scotchbond Universal Plus – SU (3M ESPE, 12290131, 29-07-2028, Neuss, Alemanha) e o Futurabond M+ – FM (VOCO, 2530433, 2027-07, Cuxhaven, Alemanha). Após o procedimento adesivo realizou-se a restauração com resina composta (grandioSO – VOCO, VC 52 002612 E1, 2028-02).
Todos os materiais foram fotopolimerizados com o fotopolimerizador (COXO, DB-686, Guangdong, China). Os espécimes foram preparados para visualização da interface adesiva por microscopia eletrónica de varrimento. As fotomicrografias foram obtidas a 15 kV, distância de trabalho 13 mm, com ampliações 500 X e 1500 X.
Resultados: No SU sem PNTA observou-se camada híbrida contínua e homogénea, com prolongamentos de resina finos e túbulos dentinários bem definidos; com a aplicação de PNTA verificou-se aumento do número e do comprimento dos prolongamentos. No FM, com e sem PNTA, a camada híbrida foi mais fina e
moderadamente uniforme, com discretas variações de espessura.
Conclusões: O PNTA não provocou alterações morfológicas relevantes na formação da camada híbrida. As diferenças dependeram sobretudo do adesivo, sendo o SU o que apresentou interface mais homogénea e melhor integração com a dentina, sugerindo maior influência do sistema adesivo na qualidade da interface adesiva do que a utilização do PNTA.
Objective: To study the ultrastructure of the hybrid layer formed between two universal adhesive systems and dentin, with and without prior application of argon non-thermal atmospheric plasma (NTAP), before adhesive application. Materials and Methods: Eight intact human molars from the tooth bank of Egas Moniz School of Health and Science were used. From each tooth, a mid-coronal dentin slice was obtained using a hard-tissue microtome (Accutom-50, Struers, Ballerup, Denmark) and then divided into two halves to perform the restorative procedure with the same adhesive system, differing only in whether NTAP was applied beforehand. The adhesive systems evaluated were Scotchbond Universal Plus – SU (3M ESPE, 12290131, 29-07-2028, Neuss, Germany) and Futurabond M+ – FM (VOCO, 2530433, 2027-07, Cuxhaven, Germany). After the adhesive procedure, restoration was performed with a resin composite (grandioSO – VOCO, VC 52 002612 E1, 2028-02). All materials were light-cured using a curing unit (COXO, DB-686, Guangdong, China). Specimens were prepared for evaluation of the adhesive interface by scanning electron microscopy (SEM). Photomicrographs were obtained at 15 kV, with a working distance of 13 mm, at 500× and 1500× magnifications. Results: In SU without NTAP, a continuous and homogeneous hybrid layer was observed, with thin resin tags and well-defined dentinal tubules; with NTAP application, an increase in the number and length of resin tags was noted. In FM, with and without NTAP, the hybrid layer was thinner and moderately uniform, showing slight variations in thickness. Conclusions: NTAP did not produce relevant morphological changes in hybrid layer formation. Differences were mainly related to the adhesive system, with SU showing a more homogeneous interface and better integration with dentin, suggesting that the adhesive system has a greater influence on adhesive interface quality than NTAP use.
Objective: To study the ultrastructure of the hybrid layer formed between two universal adhesive systems and dentin, with and without prior application of argon non-thermal atmospheric plasma (NTAP), before adhesive application. Materials and Methods: Eight intact human molars from the tooth bank of Egas Moniz School of Health and Science were used. From each tooth, a mid-coronal dentin slice was obtained using a hard-tissue microtome (Accutom-50, Struers, Ballerup, Denmark) and then divided into two halves to perform the restorative procedure with the same adhesive system, differing only in whether NTAP was applied beforehand. The adhesive systems evaluated were Scotchbond Universal Plus – SU (3M ESPE, 12290131, 29-07-2028, Neuss, Germany) and Futurabond M+ – FM (VOCO, 2530433, 2027-07, Cuxhaven, Germany). After the adhesive procedure, restoration was performed with a resin composite (grandioSO – VOCO, VC 52 002612 E1, 2028-02). All materials were light-cured using a curing unit (COXO, DB-686, Guangdong, China). Specimens were prepared for evaluation of the adhesive interface by scanning electron microscopy (SEM). Photomicrographs were obtained at 15 kV, with a working distance of 13 mm, at 500× and 1500× magnifications. Results: In SU without NTAP, a continuous and homogeneous hybrid layer was observed, with thin resin tags and well-defined dentinal tubules; with NTAP application, an increase in the number and length of resin tags was noted. In FM, with and without NTAP, the hybrid layer was thinner and moderately uniform, showing slight variations in thickness. Conclusions: NTAP did not produce relevant morphological changes in hybrid layer formation. Differences were mainly related to the adhesive system, with SU showing a more homogeneous interface and better integration with dentin, suggesting that the adhesive system has a greater influence on adhesive interface quality than NTAP use.
Descrição
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Palavras-chave
Dentina Camada híbrida Plasma não térmico atmosférico Microscopia eletrónica de varrimento