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Publication
A importância dos vasos do tipo H nas condições inflamatórias da gengiva e sua caracterização
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Abstract(s)
Introdução: Em patologias gengivais, como a periodontite e a peri-implantite, existem alterações ao nível do tecido ósseo. Estudos recentes referiram um novo tipo de neovasos, os vasos H, estes têm sido associados a fenómenos de regeneração óssea.
Objetivo primário: Observar histologica e ultraestruturalmente amostras gengivais de peri-implantite e periodontite.
Objetivo secundário: Observar células endoteliais para posterior identificação dos vasos H.
Materiais e Métodos: Foram colhidas amostras gengivais de peri-implantite (n=10) e de periodontite (n=3), esta colheita foi realizada para fins terapêuticos. Algumas das amostras foram preparadas para microscopia ótica (MO), e as restantes para microscopia eletrónica de transmissão (MET). Para microscopia ótica, as amostras foram fixadas em formol a 10%, desidratadas em álcool com concentrações ascendentes, incluídas em
parafina, cortadas com um micrótomo e coradas por hematoxilina – eosina. Os cortes foram observados em microscópio ótico Olympus® CX41, com ampliações de 10x, 20x e de 40x. As amostras foram fotografadas com a câmera DFK 33UX262 acoplada ao adaptador Olympus® U-CMAD3, utilizando o software IC Capture 2.5. As restantes amostras foram preparadas para serem observadas em MET de acordo com os protocolos
standard. Os cortes semi-finos foram realizados com faca de vidro e os ultrafinos com faca de diamante. As observações foram realizadas no MET JEOL 1200EX.
Resultados: Tanto na periodontite, como na peri-implantite, foram observadas áreas de infiltrado inflamatório. Em MO observaram-se pequenos vasos com algum infiltrado inflamatório em redor dos mesmos e através de MET observaram-se células endoteliais com espessamentos/duplicações da membrana basal e evidenciaram-se fibroblastos, macrófagos e outras células inflamatórias, tais como linfócitos e plasmócitos.
Conclusões: As amostras de peri-implantite evidenciaram maior infiltrado inflamatório comparativamente às amostras de periodontite. As células endoteliais apresentaram alterações ultraestruturais em ambas as patologias.
Introduction: In gingival pathologies such as periodontitis and peri-implantitis, there are changes in bone tissue. Recent studies have reported a new type of neovessel, the Hvessel, which has been associated with bone regeneration phenomena. Main aim: Histological and ultrastructural observation of gingival samples of periimplantitis and periodontitis. Secondary aim: Observe endothelial cells for later identification of H vessels. Materials and Methods: Gingival samples from peri-implantitis (n=10) and periodontitis (n=3) were collected for therapeutic purposes. Some of the samples were prepared for optical microscopy (OM) and the rest for transmission electron microscopy (TEM). For optical microscopy, the samples were fixed in 10% formaldehyde, dehydrated in alcohol with ascending concentrations, embedded in paraffin, cut with a microtome and stained with haematoxylin-eosin. The sections were observed under an Olympus® CX41 optical microscope at 10x, 20x and 40x magnification. The samples were photographed with the DFK 33UX262 camera coupled to the Olympus® U-CMAD3 adapter, using IC Capture 2.5 software. The remaining samples were prepared for observation in TEM according to standard protocols. Semi-thin sections were cut with a glass knife and ultra-thin sections with a diamond knife. The observations were made on the JEOL 1200EX TEM. Results: Both periodontitis and peri-implantitis showed areas of inflammatory infiltrate. In OM, small vessels were observed with some inflammatory infiltrate around them and TEM showed endothelial cells with thickening/doubling of the basement membrane and evidence of fibroblasts, macrophages and other inflammatory cells such as lymphocytes and plasma cells. Conclusions: The peri-implantitis samples showed a greater inflammatory infiltrate than the periodontitis samples. The endothelial cells showed ultrastructural alterations in both pathologies.
Introduction: In gingival pathologies such as periodontitis and peri-implantitis, there are changes in bone tissue. Recent studies have reported a new type of neovessel, the Hvessel, which has been associated with bone regeneration phenomena. Main aim: Histological and ultrastructural observation of gingival samples of periimplantitis and periodontitis. Secondary aim: Observe endothelial cells for later identification of H vessels. Materials and Methods: Gingival samples from peri-implantitis (n=10) and periodontitis (n=3) were collected for therapeutic purposes. Some of the samples were prepared for optical microscopy (OM) and the rest for transmission electron microscopy (TEM). For optical microscopy, the samples were fixed in 10% formaldehyde, dehydrated in alcohol with ascending concentrations, embedded in paraffin, cut with a microtome and stained with haematoxylin-eosin. The sections were observed under an Olympus® CX41 optical microscope at 10x, 20x and 40x magnification. The samples were photographed with the DFK 33UX262 camera coupled to the Olympus® U-CMAD3 adapter, using IC Capture 2.5 software. The remaining samples were prepared for observation in TEM according to standard protocols. Semi-thin sections were cut with a glass knife and ultra-thin sections with a diamond knife. The observations were made on the JEOL 1200EX TEM. Results: Both periodontitis and peri-implantitis showed areas of inflammatory infiltrate. In OM, small vessels were observed with some inflammatory infiltrate around them and TEM showed endothelial cells with thickening/doubling of the basement membrane and evidence of fibroblasts, macrophages and other inflammatory cells such as lymphocytes and plasma cells. Conclusions: The peri-implantitis samples showed a greater inflammatory infiltrate than the periodontitis samples. The endothelial cells showed ultrastructural alterations in both pathologies.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Gengivite Periodontite Peri-implantite Vasos do tipo H