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Avaliação comparativa da atividade antimicrobiana de óleos essenciais com diferentes medicamentos intracanais endodônticos : estudo in vitro
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Abstract(s)
Objetivo: Avaliar a capacidade antibacteriana e antifúngica dos óleos essenciais de mirra e lavanda e compará-los com medicamentos intracanal.
Materiais e métodos: A atividade antimicrobiana de óleos essenciais de diferentes marcas (Pranarom, Pleno Natura e Ladrôme) e de medicamentos intracanais (clorexidina, hidróxido de cálcio) foi verificada contra Enterococcus faecalis e Candida albicans utilizando o método de difusão em ágar. Em seguida, foram efectuadas medições da concentração mínima inibitória (CMI) utilizando o método de microdiluição em placas de 96 poços, a fim de avaliar com maior precisão a eficácia das diferentes marcas de óleos essenciais.
Resultados : Os testes de difusão em disco mostraram um efeito antimicrobiano moderado dos óleos essenciais. Em contraste, a clorexidina mostrou halos de inibição muito maiores, confirmando a sua eficácia superior em comparação com os óleos essenciais e o hidroxido de cálcio nao mostrou nenhum halo.
Nos testes de microdiluição em poço, a lavanda apresentou CMIs de 0,4 a 0,6 mg/ml contra C. albicans, enquanto a mirra apresentou CMIs de 2,3 a 12,9 mg/ml. Para E. faecalis, as CMIs dos óleos de lavanda (PL e LL) e mirra (LM e PM) variaram entre 50 e 100 mg/ml, mostrando pouca inibição.
Conclusão : Este estudo destacou a importância de continuar a explorar as aplicações dos óleos essenciais em endodontia. Embora as suas propriedades naturais e benefícios potenciais, como um menor risco de desenvolvimento de resistências microbianas e propriedades ansiolíticas, sejam promissores, a sua eficácia como tratamento principal ainda necessita de mais evidências científicas e estudos clínicos aprofundados. No entanto, os óleos essenciais podem constituir uma alternativa complementar interessante em estratégias de tratamento endodontico integradas, visando maximizar a eficácia enquanto minimizam os efeitos secundários e as resistências microbianas.
Objective : This in vitro experiment aims to evaluate the antibacterial and antifungal power of myrrh and lavender essential oils and compare them with intracanal medications. Materials and Methods : The antimicrobial activity of essential oils from different brands (Pranarom, Pleno Natura and Ladrôme) and intracanal medicines (chlorhexidine, calcium hydroxide) was checked against Enterococcus faecalis and Candida albicans using the agar diffusion method. Minimum inhibitory concentration (MIC) measurements were then carried out using the microdilution method in 96-well plates in order to more accurately assess the efficacy of the different brands of essential oils. Results : The disk diffusion tests showed a moderate antimicrobial effect of the essential oils. In contrast, chlorhexidine showed much larger inhibition halos, confirming its superior efficacy compared to the essential oils and calcium hydroxide showed no halo. In the microdilution well tests, lavender showed MICs of 0.4 to 0.6 mg/ml against C. albicans, while myrrh showed MICs of 2.3 to 12.9 mg/ml. For E. faecalis, the MICs of lavender (PL and LL) and myrrh (LM and PM) oils ranged from 50 to 100 mg/ml, showing little inhibition. Conclusion : This study highlighted the importance of continuing to explore the applications of essential oils in endodontics. Although their natural properties and potential benefits, such as a lower risk of developing microbial resistance and anxiolytic properties, are promising, their effectiveness as a primary treatment still requires more scientific evidence and in-depth clinical studies. However, essential oils can constitute an interesting complementary alternative in integrated endodontic treatment strategies, aiming to maximize effectiveness while minimizing side effects and microbial resistance.
Objective : This in vitro experiment aims to evaluate the antibacterial and antifungal power of myrrh and lavender essential oils and compare them with intracanal medications. Materials and Methods : The antimicrobial activity of essential oils from different brands (Pranarom, Pleno Natura and Ladrôme) and intracanal medicines (chlorhexidine, calcium hydroxide) was checked against Enterococcus faecalis and Candida albicans using the agar diffusion method. Minimum inhibitory concentration (MIC) measurements were then carried out using the microdilution method in 96-well plates in order to more accurately assess the efficacy of the different brands of essential oils. Results : The disk diffusion tests showed a moderate antimicrobial effect of the essential oils. In contrast, chlorhexidine showed much larger inhibition halos, confirming its superior efficacy compared to the essential oils and calcium hydroxide showed no halo. In the microdilution well tests, lavender showed MICs of 0.4 to 0.6 mg/ml against C. albicans, while myrrh showed MICs of 2.3 to 12.9 mg/ml. For E. faecalis, the MICs of lavender (PL and LL) and myrrh (LM and PM) oils ranged from 50 to 100 mg/ml, showing little inhibition. Conclusion : This study highlighted the importance of continuing to explore the applications of essential oils in endodontics. Although their natural properties and potential benefits, such as a lower risk of developing microbial resistance and anxiolytic properties, are promising, their effectiveness as a primary treatment still requires more scientific evidence and in-depth clinical studies. However, essential oils can constitute an interesting complementary alternative in integrated endodontic treatment strategies, aiming to maximize effectiveness while minimizing side effects and microbial resistance.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Óleo essencial Antibacteriano Antifúngico Endodontia