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Publication
Photobiology of lightsensitive transcription factors
| Name: | Description: | Size: | Format: | |
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| 4.75 MB | Adobe PDF |
Abstract(s)
Protein structure and function is a major research area in life sciences. A novel field
emerged since the discovery of proteins that are photosensitive. These photoreceptor proteins
undergo light-induced structural changes, performing the most varied tasks and contributing
to the operation of a large number of biological processes.
The importance of this type of proteins is because in the near future it will possible to
use them in different contexts and in a way that is beneficial to humans.
The final curricular internship of the Master in Biological and Chemical Engineering was
held at the Institute of Biotechnology of the BIOCEV research center located in Vestec, Czech
Republic. I was received for a period of 5 months and was part of a multicultural team working
on a project entitled Dynamics of Biological Processes .
Throughout these 5 months of Master's degree internship, the main objective was to
conduct a deeper study on the photoinduced changes of CarH and EL222 proteins. For both
proteins, it is important to discover the conformational changes that occur between the dark
state and the light state
A realistic work plan was developed that is appropriate to the capabilities of both the
team and the research center. At the end of the internship period, significant results were
obtained . In particular, I demonstrated the possibility of introducing non-canonical amino acids
in EL222 with sufficient yield for future infrared spectroscopy experiments. I also showed the
feasibility of studying CarH structural dynamics by infrared spectroscopy.
Description
Keywords
Protein structure and dynamics Photoreceptors Protein-DNA interactions Noncanonical amino acids Infrared spectroscopy