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Feeding strategies for tuning poly (3-hydroxybutyrate-co-4-hydroxybutyrate) monomeric composition and productivity using Burkholderia sacchari

2017-12Journal article Restricted access
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dc.contributor.authorRaposo, Rodrigo S.
dc.contributor.authorAlmeida, M. Catarina M. D. de
dc.contributor.authorFonseca, M. M. R. da
dc.contributor.authorCesário, M. Teresa
dc.date.accessioned2019-12-09T16:26:41Z
dc.date.available2019-12-09T16:26:41Z
dc.date.issued2017-12
dc.description.abstractPoly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-4HB)) co-polymers were produced at bench-scale in fed-batch cultivations by Burkholderia sacchari from glucose (main carbon-source) and gamma-butyrolactone (GBL) as co-substrate. As P(3HB-4HB) properties highly depend on the 4-hydroxybutyrate (4HB) molar fraction, it is advantageous to have a thorough knowledge of the process in order to promote the production of the targeted final product. In this work, polymers with a 4HB molar percentage ranging from 1.5 to 8.4% (mol/mol) were obtained as consequence of a fine tuning of the fed-batch operation conditions, namely regarding the co-substrate feeding rate and its addition time, as GBL is toxic to B. sacchari cells. The best results regarding both the 4HB incorporation (molar%) and the co-polymer productivity (7.1% and 1.1g/(L.h) respectively) were reached when a pulse of GBL (<10g/L) was added early in the accumulation phase followed by a constant GBL addition at a rate similar to that of consumption so that a steady co-substrate concentration in the medium was maintained.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Biol Macromol. 2017 Dec;105(Pt 1):825-833. doi: 10.1016/j.ijbiomac.2017.07.111pt_PT
dc.identifier.doi10.1016/j.ijbiomac.2017.07.111pt_PT
dc.identifier.issn0141-8130
dc.identifier.urihttp://hdl.handle.net/10400.26/30414
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNew tailor-made PHB-based nanocomposites for high performance applications produced from environmentally friendly production routes
dc.relationPRODUCTION OF POLY 3-HYDROXYBUTYRATE-CO- 4-HYDROXYBUTYRATE P 3HB-CO-4HB FROM LIGNOCELLULOSIC AGRO-INDUSTRIAL WASTES
dc.relationPRODUÇÃO DE POLIHIDROXIALCANOATOS UTILIZANDO SUBSTRATOS RESIDUAIS
dc.relation.publisherversionhttps://doi.org/10.1016/j.ijbiomac.2017.07.111pt_PT
dc.subjectBurkholderia sacchaript_PT
dc.subjectPoly (3-hydroxybutyrate-co-4-hydroxybutyrate)pt_PT
dc.subjectP(3HB-4HB)pt_PT
dc.subjectGamma-butyrolactonept_PT
dc.subjectBioreactor feeding strategypt_PT
dc.titleFeeding strategies for tuning poly (3-hydroxybutyrate-co-4-hydroxybutyrate) monomeric composition and productivity using Burkholderia sacchaript_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleNew tailor-made PHB-based nanocomposites for high performance applications produced from environmentally friendly production routes
oaire.awardTitlePRODUCTION OF POLY 3-HYDROXYBUTYRATE-CO- 4-HYDROXYBUTYRATE P 3HB-CO-4HB FROM LIGNOCELLULOSIC AGRO-INDUSTRIAL WASTES
oaire.awardTitlePRODUÇÃO DE POLIHIDROXIALCANOATOS UTILIZANDO SUBSTRATOS RESIDUAIS
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/246449/EU
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIO%2F04565%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F68587%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F26678%2F2006/PT
oaire.citation.endPage833pt_PT
oaire.citation.startPage825pt_PT
oaire.citation.titleInternational Journal of Biological Macromoleculespt_PT
oaire.citation.volume105(part 1)pt_PT
oaire.fundingStreamFP7
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctPolítica de copyright da editorapt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicatione88d1819-1f6d-4b1d-a864-ec2091b17e8a
relation.isProjectOfPublication05007b22-49a3-4d43-b16d-8ad72ab05be7
relation.isProjectOfPublication8b03dfca-bdb8-448e-8333-cbd672ab33ee
relation.isProjectOfPublication54143930-0087-45be-ae76-b932ef0f1af1
relation.isProjectOfPublication.latestForDiscovery05007b22-49a3-4d43-b16d-8ad72ab05be7

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