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Comprehensive chemical and enantiomeric characterization of commercial turpentine oils by GC–MS
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Orientador(es)
Resumo(s)
Recent reports from families of individuals with Angelman syndrome (AS), a rare neurodevelopmental disorder characterised by severe neurological impairment and epilepsy, have described perceived neurological improvements following the topical application of a commercial turpentine oil (TO). These observations prompted the present analytical study, which aimed to characterise and quantify the terpenoid composition of 2 commercial TOs (Diamond and Creekwood), together with 6 additional commercial TOs, using gas chromatography-mass spectrometry (GC–MS). Analysis of 6 TOs reported to originate from Pinus pinaster revealed similar combined percentages of α- and β-pinene (≈75%), except for one oil that exhibited a more complex profile, a lower α-pinene content (≈30%), and the presence of eucalyptol, which is uncommon in TOs from this species. The Creekwood oil contained a higher proportion of α-pinene (≈76%) and a markedly lower β-pinene content (≈3%) than the Diamond oil (≈62% and ≈26%, respectively). Chiral GC–MS analysis further revealed contrasting enantiomeric distributions, with (–)-α-pinene predominating in the Diamond oil and (+)-α-pinene in the Creekwood oil, while (–)-β-pinene predominated in both samples. For quantitative analysis, the analytical method was successfully validated, demonstrating suitable detection (1.5–5.0 mg/L) and quantification (5.0–16.7 mg/L) limits, good linearity (r2>0.991), and acceptable precision and accuracy (<15%). These findings demonstrate substantial chemical variability among commercial TOs and provide a framework for future studies on the biological effects of pine resin terpenoids in neuronal models relevant to AS.
Descrição
Palavras-chave
Turpentine oils Terpenoids Angelman syndrome Gas chromatography-mass spectrometry Validation
Contexto Educativo
Citação
Débora․S․ D․ Pombo, Bárbara F․ C․ Barra, Madalena Bettencourt, Alexandre Quintas, Evguenia P. Bekman, Nuno R. Neng, Comprehensive chemical and enantiomeric characterization of commercial turpentine oils by GC–MS, Journal of Chromatography A, Volume 1785, 2026, 467289, https://doi.org/10.1016/j.chroma.2026.467289
Editora
Elsevier