Percorrer por autor "Trindade, Alexandre"
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- Aplicação de medidas ambientais na Academia Militar-Sede: impacto económicoPublication . Trindade, AlexandreAs Forças Armadas no geral, e o Exército em particular, têm procurado agir em conformidade com a política ambiental do Governo, cooperando em prol da preservação do ambiente e de um desenvolvimento sustentável. Neste âmbito, surge a tentativa de identificar qual a realidade ambiental existente na Academia Militar-Sede, instituição dedicada à formação dos futuros oficiais do Exército e da Guarda Nacional Republicana. Deste modo, a investigação tem como objectivo avaliar o impacto económico consequente à adopção de determinadas medidas ambientais, de acordo com a realidade envolvente. O presente trabalho foi dividido em duas partes distintas: a primeira referindo-se ao enquadramento teórico e a segunda contendo os resultados obtidos através do trabalho de campo. Relativamente à primeira fase, foi realizada uma recolha de dados para a prossecução dos objectivos anteriormente referidos, que se consubstanciou essencialmente, numa análise documental decorrente de uma exaustiva pesquisa bibliográfica. Numa segunda fase, elaboraram-se entrevistas e inquéritos, e analisaram-se as propostas referentes à implementação de medidas de protecção ambiental, com o objectivo de avaliar a sua viabilidade económica. De acordo com a análise dos dados recolhidos pelos supracitados métodos de investigação, concluiu-se que há actualmente, um fraco desempenho ambiental por parte da Academia Militar-Sede, razão pela qual se considera urgente a criação de uma política que defina claramente orientações no sentido de reverter a situação actual. Estas deverão incidir num consumo de energia e de água mais eficientes, na separação correcta de resíduos, nascompras públicas ambientalmente orientadas e na formação e sensibilização dos indivíduos que diariamente, frequentam o espaço da Academia Militar-Sede. No que respeita ao impacto económico subjacente a estas medidas, comprovou-se que elas estão intimamente ligadas à obtenção de novas fontes de receitas, devendo pois, ser encaradas como prioridades a definir.
- Bacteriostatic and antibiofilm efficacy of a Nisin Z solution against co-cultures of Staphylococcus aureus and Pseudomonas aeruginosa from diabetic foot infectionsPublication . Serrano, Isa; Alhinho, Bernardo; Cunha, Eva; Tavares, Luís; Trindade, Alexandre; Oliveira, ManuelaDiabetes mellitus (DM) patients frequently develop diabetic foot ulcers (DFU) which are generally infected by a community of microorganisms, mainly Staphylococcus aureus and Pseudomonas aeruginosa. These bacteria exhibit a multi-drug resistance profile and biofilm-forming ability which represent a hurdle in the treatment of diabetic foot infections (DFI). We aimed to evaluate the potential of Nisin Z, an antimicrobial peptide (AMP), as an alternative treatment for severe DFI. Nisin Z shows antibacterial activity against Gram-positive and Gram-negative bacteria and an increased antibacterial effect against Gram-negatives when added to EDTA. As such, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), Minimum Biofilm Inhibitory Concentration (MBIC), and Minimum Biofilm Eradication Concentration (MBEC) were determined for Nisin Z, Nisin Z + EDTA (0.4%), and Nisin Z + EDTA incorporated into guar gum, in order to test its efficacy against S. aureus and P. aeruginosa isolated from the same DFU. Results showed that Nisin Z added to the chelation agent EDTA displayed higher antibacterial and bacteriostatic efficacy against mono and dual co-cultures of S. aureus and P. aeruginosa, and higher antibiofilm efficiency against monocultures. Nisin Z was moderately cytotoxic at 200 µg/mL. Prospect in vivo studies are needed to confirm the potential of Nisin Z supplemented with EDTA to be used as a complement to conventional antibiotic therapy for severe DFI.
- From waste to resource : performance of Black Soldier fly larvae reared on restaurant food waste at an industrial scalePublication . Oliveira, Joana; Ligeiro, Carolina; Fantatto, Rafaela; Souza, Clarice Silva e; Machado, Maria Ana; Guilgur, Leonardo Gaston; Trindade, Alexandre; Assunção, Ricardo; Murta, DanielFood waste poses significant environmental, economic and public health challenges. The black soldier fly (Hermetia illucens) larvae (BSFL) represent a promising solution for organic waste valorisation, converting substrates into protein-rich biomass for animal feed and organic fertiliser. However, the use of food waste as an insect substrate remains prohibited in the European Union due to regulatory and safety concerns. This study evaluated the suitability of heterogeneous food waste for BSFL rearing under industrial conditions by comparing larval performance on a standard Gainesville diet (control) and a blend derived from local restaurant waste (test). The 14-day bioconversion assay assessed bioconversion rate (BCR), feed conversion ratio (FCR), survival rate, average growth rate, and nutritional composition. Compared with the control, the test group showed significantly improved (p < 0.001) BCR (18.34% vs. 11.02%), FCR (5.48 vs. 9.09 kg/kg), survival (69.29% vs. 51.30%), and growth (8.38 vs. 6.59 mg/day). Larvae reared on food waste also exhibited significantly higher protein (19.70% vs. 16.80%), fat (13.70% vs. 7.20%), ash (6.97% vs. 3.51%), carbohydrates (7.00% vs. 3.60%), and fibre (5.20% vs. 2.90%). Overall, heterogeneous food waste is a suitable substrate for BSFL, supporting agrifood sustainability; however, future research should focus on standardisation of these substrates.
- Genomic insights and bioconversion potential in the Black Soldier fly (Hermetia illucens) : current advances and future directionsPublication . Oliveira, Joana; Guilgur, Leonardo Gaston; Assunção, Ricardo; Murta, Daniel; Trindade, AlexandreThe global population continues to rise, placing increasing pressure on the agri-food sector and leading to the accelerated generation of urban organic waste, factors that collectively intensify climate stress and environmental instability. Insects are recognised for their remarkable capacity to transform substrates into valuable products, with the black soldier fly larvae (BSFL) emerging as one of the most efficient and widely utilised species for this purpose. Beyond recycling organic matter, BSFL can also mitigate microbial contamination, effectively reducing bacterial and fungal loads in waste substrates. Understanding and manipulating the genome could provide tools to improve BSFL bioconversion process and contribute to sustainability. In this review, we provide an overview of recent advances in black soldier fly genomics and genome-editing technologies. Although research in this subject remains limited, recent studies have clarified its origin, characterised its genome, and established the foundation for targeted genetic improvements to enhance by-product conversion, nutrient recovery, and environmental sustainability.