Percorrer por autor "Ribeiro, T."
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- Assessment of chemical hazards in insect meal production for aquaculture feedsPublication . Amaral, I.; Ahmad, S. M.; Ângelo, M.; Correia da Silva, P.; Quintas, A.; Brito, J. A. A.; Murta, D.; Santos, M. V.; Vieira, I.; Ribeiro, T.; Gonçalves, L. L.
- Growth performance, bioconversion efficiency, and nutritional quality of yellow mealworm larvae reared on formulated diets based on local agro-industrial by-productsPublication . Loiotine, Z.; Oddon, S. Bellezza; Resconi, A.; Murta, Daniel; Ribeiro, T.; Biasato, I.; Gasco, L.Studies on yellow mealworm (YM, Tenebrio molitor) have mainly used feeding substrates with heterogeneous nutritional composition. In this study, three multi-ingredient agro-industrial diets (YM1, YM2, YM3), formulated to be nutritionally comparable in terms of CP (19.0–19.6% DM), ether extract (EE 3.0–3.2% DM), and gross energy (17.7–18.5 MJ/Kg DM), were tested to evaluate their effects on larval growth, bioconversion, chemical composition, and economic affordability. Diet YM1 included wheat middlings, wafer dough cooked, dry distillery stillage, coffee silvery film, breading waste and feed waste. Diet YM2 was based on feed waste and breading waste, with minor inclusions of wafer dough cooked and sweet preparation, whereas YM3 consisted of feed waste, breading waste and rice by-products (rice husk, chaff and bran). Wheat bran (WB; CP 20.5%, EE 4.2%, gross energy 18.6 MJ/kg, DM) served as environmental control. Diet costs (€/ton) calculated from by-product inclusion level and market price were 87.80 (YM1), 83.30 (YM2), and 95.50 (YM3), when compared to €250.00/tonne for WB. Four-week-old YM larvae (10 000/tray) were reared on 3 kg of diet per replicate and sampled weekly until growth differences between consecutive samplings were < 50% (week 9 of age, 35 days). Agar (25 g/L) was supplied three times weekly. At the end of the larval growth and biomass, development time, survival, growth rate (GR, mg/day), specific growth rate (SGR, %/day), feed conversion ratio, efficiency of conversion of ingested food (%), feed intake and water intake (%) were recorded. Statistical analyses were performed using IBM SPSS (v.20.0; P < 0.05). Rearing tray was the experimental unit with four replicates per treatment. A one-way ANOVA with Tukey’s post-hoc test was applied for all the tested parameters. At 9 weeks of age, larvae fed YM2 and YM3 showed significantly higher final average weight (139 mg for both YM diets), GR (3.90–3.91 mg), and SGR (11.90% for both) than those fed YM1 (118 mg; 3.31 mg–11.40%, respectively; P < 0.001), with no differences between YM2 and YM3 (P > 0.05). Larval composition varied across treatments (P < 0.05), with higher DM and EE in YM1 (36.50−37.90%, respectively) than YM3 (35.40–35.0%), while YM2 showed no significant differences (36.0–37.3%). Ash content was higher in YM3 (4.02%) than in YM1 and YM2 (3.34–3.45%; P < 0.05). In conclusion, nutritionally comparable multi-ingredient agro-industrial diets supported YM growth and bioconversion, while reducing feed costs when compared to the WB diet.
- QUANTIFILER®TRIO DNA method performance in a collection of ancient samplesPublication . Vieira- Silva, C.; Lopes, J.; Afonso Costa, H.; Ribeiro, T.; Porto, M.J.; Dias, M; Cunha, E; Amorim, A,During the past few years significant progress has been made in solving technical challenges associated with STR profiling including the ability to analyze degraded DNA and low amounts of DNA. The result of these changes is that useful STR profiles can now be obtained from previously untypeable forensic DNA samples. Analysis of DNA from ancient material represents an important role in molecular anthropology, although there are many limitations concerning low DNA quantity and/or degraded DNA, and/or PCR inhibitors. These factors can make it difficult to decide whether to continue with STR analysis, which STR panel to use and how much DNA to add to PCR reaction. With all these constraints, DNA quantification represents an important tool to decide which method will follow in order to improve workflow and have good results in less time-consuming. The Quantifiler® Trio DNA method provides a quality index (QI) to detect the presence of degraded DNA along with PCR inhibitors.This guide allows the selection of the optimal short tandem repeat (STR) analysis chemistry (autosomal, or miniSTR) and streamlines the workflow while increasing downstream analysis success rates. In order to compare DNA quality from different extraction methods, samples from 46 exhumed Middle Ages individuals were extracted with modified phenol-chloroform method and also PrepFiler Express BTA™ method. DNA was quantified with Quantifiler® Trio DNA Quantification in an Applied Biosystems® 7500 Real-Time PCR System. Results were analyzed and allow us to point Quantifiler® Trio method as an important tool in pre-STR typing methods in ancient samples
- Usefulness of the Powerplex1 Fusion System as typing methodology for CODIS and ESS genetic markers in an immigrant population from Cabo Verde in LisboaPublication . Resende, A.; Amorim, A.; Silva, C. Vieira da; Ribeiro, T.; Porto, M. J.; Santos, J. Costa; Costa, H. Afonso"Cabo Verde is located on the West African coast, was discovered uninhabited by Portuguese explorers, colonized by European and African slaves and is culturally characterized by a mixture of their settlers. Cabo Verde is one of the countries with the highest number of emigrants living in Portugal. In order to validate the genetic markers included in the Combined DNA Index System and in the European Standard Set, samples from 500 unrelated individuals from Cabo Verde, currently living in Lisboa, were studied with the PowerPlex® Fusion System. To implement the genotyping method at the Serviço de Genética e Biologia Forenses (SGBF) we determined the minimum final volume of the PCR reaction, the proper number of PCR cycles and the minimum DNA concentration to obtain a complete genetic profile. Stutter identification and its proportion and allelic imbalance within markers were also determined. Results were compared with genetic profiles obtained by the current PCR kits used in SGBF routine testing verifying concordance between common loci. There were no off-ladder alleles detected and it was possible to type microvariants which were not present in the allelic ladders of other PCR kits. PowerPlex® Fusion System allows for the simultaneous characterization of Combined DNA Index System and in the European Standard Set genetic markers, in an economically way, with greater sensitivity and solid results."