Percorrer por autor "Noronha, Susana"
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- Association between IL-1A and IL-1B gene polymorphisms with peri-implantitis in a Portuguese population : a pilot studyPublication . Cardoso, José Maria; Ribeiro, Ana Clara; Palos, Constança; Proença, Luís; Noronha, Susana; Alves, Ricardo CastroBackground: Scientific evidence indicates that biological complications in dental implants tend to be concentrated in a subset of individuals, which seems to imply that the host response may play a determining role in implant success. Over the last few decades, several polymorphisms have been studied. Polymorphisms in the interleukin (IL) 1 gene cluster have been associated with periodontitis. There are some similar features in the sequence of immunopathological events in peri-implant and periodontal infections. We aimed to investigate if individuals carrying the genetic single nucleotide polymorphism (SNP) in the IL-1A (rs1800587) and IL-1B (rs1143634) genes are more susceptible to develop peri-implantitis. Methods: A cross-sectional analytic pilot study was conducted in 20 Caucasian Portuguese subjects divided into two groups: 10 subjects with peri-implantitis and 10 subjects with peri-implant health (control group). Samples containing cells from the buccal mucosa were stored at −20 °C and later submitted to the DNA extraction process. Genetic analysis was performed using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. Data were analyzed by using descriptive and inferential statistical methodologies. Results: For the IL-1A (−889) gene polymorphism, it was observed that the mutated allele was present in a higher percentage in the peri-implantitis group compared to the control group (30% vs 15% respectively, Fisher’s exact test, p = 0.45). For the IL-1B (+3954) gene polymorphism, it was also observed that the altered allele was present in a higher percentage in the disease group compared to the control group (35% vs 10% respectively, Fisher’s exact test, p = 0.13). The positive genotype (at least one allele with nucleotide sequence changed in both genes) was detected in six patients, five belonging to the disease group and one to the health group. Conclusions: Regarding IL-1 gene polymorphisms, there was no statistically significant difference between the health and disease group, however a trend should be highlighted, showing a potential link between the IL-1 genotype and peri-implantitis. More studies are needed to clarify the role of genetic polymorphisms in the development of peri-implantitis.
- Association between IL-1A, IL-1B and IL-1RN polymorphisms and peri-implantitis : a systematic review and meta-analysisPublication . Cardoso, José Maria; Duarte, Sofia; Ribeiro, Ana Clara; Mascarenhas, Paulo; Noronha, Susana; Alves, Ricardo CastroRecent studies report that individuals with polymorphisms in the genes that encode for interleukin (IL)-1α and IL-1β (IL-1A and IL1B, respectively) and for IL-1 receptor antagonist (IL-1RN) may be more susceptible in developing peri-implantitis. Therefore, the current systematic review evaluates what is reported about the role of genetics, more specifically of single nucleotide polymorphisms (SNP) on IL-1 and variable number of tandem repeats (VNTR) on IL-1RN, in the development of peri-implantitis. This systematic review was carried out by screening PubMed, B-on, Cochrane and Scopus databases, for articles English, Spanish, and Portuguese, with no limit regarding the publication year. Eight articles were selected for systematic review and four for meta-analytic syntheses. Our results show that although there is a lack of consensus in the literature, there seems to be an association between IL-1A, IL-1B, and IL-1RN polymorphisms with peri-implantitis. The results of the meta-analysis showed that patients who have the polymorphic allele at position +3954 of the IL-1B gene have on average almost twice the risk of developing peri-implantitis (odds ratio = 1.986, 95% confidence interval).
- Association between periodontal disease and Alzheimer’s disease : a cross-sectional studyPublication . Rodrigues, Vanessa Rocha; Amorim, Mónica; Alcoforado, Gil; Noronha, SusanaObjectives: To evaluate whether periodontal disease is more prevalent in adults diagnosed with Alzheimer's disease compared to adults without the condition. Methods: A total of 72 individuals were included in the study: 36 patients diagnosed with Alzheimer's disease (test group) and 36 matched individuals without Alzheimer's disease (control group). The diagnosis of periodontal disease was established through a comprehensive periodontal assessment. Additionally, a microbiological analysis of subgingival plaque samples was conducted to detect the main periodontal pathogens. Results: Statistically significant differences were observed in periodontitis extent between the two groups: 82.4% of patients in the test group and 56.3% in the control group presented generalized periodontitis. No association was found between the stage and extent of periodontitis and the severity of Alzheimer's disease. Furthermore, no significant associations were observed between the number of years since Alzheimer's diagnosis and periodontal parameters, including the stage (p=0.659), extent (p=0.551), and grade of periodontitis (p=0.769). Prevotella intermedia was found in 25% of the test group and 5.6% of the control group (p=0.022). Campylobacter rectus appeared in 16.7% of the cases, with a significant difference between groups (p=0.011). Fusobacterium nucleatum was more common in the control group (75%) than in the test group (27.8%), with a significant difference (p<0.001). Eikenella corrodens was detected only in the control group (22.2%) (p=0.005). Conclusions: Although the overall prevalence of periodontitis was similar between groups, individuals with Alzheimer’s disease tended to present a greater extent of generalized periodontitis.
- The influence of genetic polymorphisms on the expression of interleukin-1beta, prostaglandin E2 and tumor necrosis factor alpha in peri-implant crevicular fluid : a cross-sectional studyPublication . Cardoso, José Maria; Ribeiro, Ana Clara; Botelho, João; Proença, Luís; Noronha, Susana; Alves, Ricardo CastroThe aim of this study was to evaluate the possible relationships between polymorphisms in the interleukin-1 (IL-1) A, IL-1B, and IL-1RN genes and concentrations of the inflammatory mediators IL-1β, tumor necrosis factor-alpha (TNF-α), and prostaglandin E2 (PGE2) in peri-implant crevicular fluid (PICF). A cross-sectional analytical study was conducted on 51 patients with dental implants. Samples from the buccal mucosa were obtained, and genetic analysis was performed using the real-time polymerase chain reaction (PCR) technique for IL-1A and IL-1B and PCR and restriction fragment length polymorphism analysis for IL-1RN. For the biochemical analysis, the concentrations of IL-1β and TNF-α were analyzed using multiplexed fluorescent sphere immunoassays, and PGE2 by enzyme-linked immunosorbent assay. In patients with detected IL-1RN polymorphism, there was an increase in the concentration of the three mediators with statistically significant differences in the mean values of TNF-α and PGE2, regardless of peri-implant health status (p = 0.002 and p = 0.049, respectively). The concentrations of all three mediators were positively and significantly correlated (IL-1β vs. TNF-α Rho = 0.480, p < 0.001; IL-1β vs. PGE2 Rho = 0.382, p = 0.006; and TNF-α vs. PGE2 Rho = 0.528, p < 0.001). We can conclude that the IL-1RN polymorphism exerts an influence on the PICF immune response, which may explain the influence of this genetic polymorphism on the occurrence of peri-implantitis.
- The role of hyaluron acid in non-surgical treatment of chronic periodontitis : systematic reviewPublication . Rodrigues, Vanessa Rocha; Rocha, Pedro; Otâo, Pedro; Luís, Henrique; Noronha, Susana; Mascarenhas, PauloObjectives: The purpose of this review is to perform a systematic evaluation of the clinical effects of the local subgingival use of 0.8% or 0.2% hyaluronic acid (HA) as an adjunct to scaling and root planning in patients with chronic periodontitis. Methods: An electronic search was performed on PubMed, Lilacs, and Cochrane, without any time or language restrictions, on May 9, 2018, using the following keywords: “hyaluronic acid”; “periodontitis”; “treatment of periodontitis”; and “scaling.” The following parameters were evaluated: clinical attachment level (CAL) gain, probing depth (PD), bleeding on probing (BOP), plaque index (PI), and gingival index (IG). This review was registered with the identification number CRD42019131541 in the PROSPERO International Prospective Register of Systematic Reviews hosted by the National Institute for Health Research, University of York, Centre for Reviews and Dissemination. Results: When applied to periodontitis, the combined treatment HA + SRP provides more satisfactory results than the conventional treatment of SRP alone in most of the outcome variables presented. Conclusion: It can be concluded that hyaluronic acid is a chemical adjuvant to consider in periodontal therapy.