Percorrer por autor "Belas, Adriana"
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- ESBL/pAmpC-producing Escherichia coli causing urinary tract infections in non-related companion animals and humansPublication . Belas, Adriana; Marques, Cátia; Menezes, Juliana; Gama, Luís Telo da; Cavaco-Silva, Patrícia; Pomba, ConstançaUrinary tract infections (UTI) caused by Escherichia coli are frequently diagnosed in humans and companion animals. Extended-spectrum beta-lactamase (ESBL)- and cephalosporinase (pAmpC)-producing Escherichia coli are worldwide-disseminated and frequently multidrug-resistant, hence leading to treatment failure and public health concerns. This study aimed to characterize and compare ESBL/pAmpC-producing E. coli strains causing community-acquired UTI in companion animals and non-related humans. Third-generation cephalosporin (3GC)-resistant E. coli (companion animals n = 35; humans n = 85) isolated from patients with UTI were tested against 14 antimicrobials following CLSI guidelines. PCR-based assays were used to detect the major E. coli phylogenetic groups, pathogenicity associated-islands (PAIs), virulence genes, and ESBLs/pAmpC resistance genes. ESBL/pAmpC-producing E. coli isolates were typed by multi-locus sequence typing (MLST) and PCR. E. coli strains from companion animals and humans shared two MDR high-risk clonal lineages: ST131 and ST648. To the best of our knowledge, this study reports the first description of E. coli ST131 clade C1-M27 and the clonal lineage ST131 clade A in humans with community-acquired UTI in Portugal. Considering that companion animals with UTI are generally treated at home by the owners, measures should be implemented to avoid the spread of multidrug-resistant high-risk clones to humans and their household environment.
- Human and companion animal Proteus mirabilis sharingPublication . Marques, Cátia; Belas, Adriana; Menezes, Juliana; Silva, Joana Moreira da; Cavaco-Silva, Patrícia; Trigueiro, Graça; Gama, Luís T.; Pomba, ConstançaProteus mirabilis is an important pathogen that is associated with urinary tract infections. This study aims to determine the colonization and sharing of P. mirabilis between healthy companion animals and humans that are living together and to evaluate the clonal relatedness of the fecal and clinical stains. Eighteen households (24 humans, 18 dogs, 8 cats) with at least one human–animal pair were studied. Fecal samples were plated onto MacConkey and Hektoen agar and P. mirabilis PFGE analysis (NotI; Dice/UPGMA; 1.5% tolerance) was conducted for the households with multiple positive participants. Antimicrobial-resistance was tested according to CLSI. The fecal P. mirabilis pulse-types were compared with uropathogenic clinical strains (n = 183). Forty-nine P. mirabilis were isolated from eight households. The percentage of colonization in the dogs (44.4%, n = 8/18) was significantly higher (p = 0.0329) than in the humans (12.5%, n = 3/24). Three households had multiple colonized participants. One human–dog pair shared related P. mirabilis strains, which clustered with a clinical strain of animal origin (82.5%). One fecal P. mirabilis strain, from a dog, clustered with two human community-acquired clinical strains (80.9%, 88.9%). To our knowledge, this is the first report of dogs and humans living in close contact and sharing related P. mirabilis strains. The high frequency of colonization in the dogs underlines their possible role as P. mirabilis reservoirs for humans and other dogs.
- Lyme disease in companion animals : an updated state-of-art and current situation in PortugalPublication . Picado, Rita; Baptista, Catarina Jota; Meneses, André; Legatti, Sabrina; Fonseca, Joana; Belas, AdrianaLyme disease (LD) is a globally distributed zoonotic multisystemic condition caused by gram-negative spirochete bacteria of the Borrelia burgdorferi complex, transmitted through tick bites. Research on LD in domestic animals in Portugal is limited, potentially leading to underestimating its prevalence. This disease affects many species, including humans, making it a critical public health issue. In domestic animals, LD often presents subclinically or with non-specific clinical signs, complicating its diagnosis. Nevertheless, veterinarians should always consider LD in cases with a history of tick exposure and compatible clinical signs. Diagnostic confirmation can be achieved through serological and other complementary tests. Treatment involves eradicating the bacterial infection and managing clinical signs using a combination of antibiotics, analgesics, anti-inflammatories, and other medications. Effective prevention primarily relies on tick control measures. This review aims to provide an up-to-date state-of-the-art LD, particularly in Portugal.
- Prevalence and genetic characterization of feline leukemia virus in portuguese stray catsPublication . Almeida, Pedro Morais de; Belas, Adriana; Bragança, Mauro; Oliveira, Joana de; Viegas, CarlosBackground: With its large stray population, Portugal had the highest prevalence of the Feline Leukemia Virus (FeLV) infection in Europe. Progressive infection is still high, especially in southern Europe. In addition to the viral load, the subgroup is a determining factor in the development of the various syndromes associated with this disease, which can be fatal, justifying the morbidity and mortality of this retrovirus. The aim of the study was to determine the prevalence of FeLV, the viral load and to identify the subgroup of circulating virus in stray cats in the Lisbon metropolitan area. This prospective study was conducted at the Veterinary Teaching Hospital of the FMV-ULusófona, from November 2022 to January 2023. Blood samples from 129 stray cats from the Lisbon metropolitan area were included. All samples were tested for p27 antigen detection and proviral DNA by qPCR. For the detection of FeLV subgroups (A, B and C) nested PCR was performed. Results: The results showed that at the time of enrollment, 8.5% (n = 11/129) of the cats tested positive for FeLV using p27 antigen and qPCR for proviral DNA. Considering the proviral DNA load, 90.9% (n = 10/11) of the FeLV infections were classified as “likely progressive infection” (> 4.0 × 105 copies/mL). In the remaining animal, the FeLV infection was classified as “likely regressive infection” (≤ 4.0 × 105 copies/mL). In this study, the most common FeLV subgroup was a combination of AB (54,5%, n = 6/11), followed by FeLV-A (45,5%, n = 5/11). FeLV-C subgroup was not detected. Conclusions: To the best of our knowledge, this is the first study to research FeLV subgroups in Portugal. The prevalence of FeLV in this group was consistent with our expectations. However, the molecular characterization differed significantly from that observed in other countries. These results suggest that stray cats may not be the main epidemiological concern.