Loading...
Publication
Efeitos da mescalina e da dietilamida do ácido lisérgico (LSD) no fluxo glicolítico de Saccharomyces cerevisiae
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 2.84 MB | Adobe PDF |
Abstract(s)
A utilização de compostos psicadélicos em contextos rituais e religiosos é multicultural e tem uma origem pré-histórica. Nas sociedades humanas ancestrais, estas substâncias eram utilizadas para alterar o estado de consciência dos xamãs, colocando-os “em contacto com a divindade”. Estas substâncias foram proibidas durante governação de Richard Nixon em meados da década de 1970, tendo como consequência a estigmatização das mesmas. Avançando para finais da época de 1980, a utilização destes compostos em contexto clínico começou a renascer, com um crescimento mais acentuado nos últimos anos. O presente trabalho visa contribuir para a compreensão de mecanismos celulares e moleculares da mescalina e do LSD utilizando um modelo celular de leveduras Saccharomyces cerevisiae. Estas leveduras apresentam mecanismos moleculares e celulares semelhantes às células humanas, traduzindo-se numa abordagem mais simples que permite compreender a bioquímica fundamental das células em diferentes condições. As metodologias empregues permitiram avaliar os parâmetros associados ao crescimento celular, bem como a sensibilidade e viabilidade celulares e a atividade metabólica na presença de concentrações crescentes de LSD e mescalina. Os resultados obtidos permitiram concluir que há um efeito exercido por ambos os compostos na atividade mitocondrial e, eventualmente, no fluxo glicolítico das leveduras estudadas. O presente trabalho mostra, pela primeira vez, um aumento do metabolismo celular na presença de LSD e mescalina. Esta observação poderá estar associada às melhorias detetadas clinicamente. Este é um estudo pioneiro e exploratório, pelo que será necessário repetir alguns ensaios para confirmar as conclusões apresentadas.
The use of psychedelic compounds in ritual and religious contexts is multicultural and has prehistoric origins. In ancient human societies, these substances were used to alter the state of consciousness of shamans, putting them “in touch with divinity”. These substances were banned during Richard Nixon’s administration in the mid-1970s, resulting in their stigmatization. Towards the end of the 1980s, the use of these compounds in a clinical context began to revive, with a more marked increase in recent years. This study aims to contribute to understanding the cellular and molecular mechanisms of mescaline and LSD using a cellular model of Saccharomyces cerevisiae yeast. This yeast has similar molecular and cellular mechanisms to human cells, making this a simpler approach to understanding the fundamental biochemistry of cells under different conditions. The methodologies used made it possible to evaluate the parameters associated with cell growth, as well as cell sensitivity, viability, and metabolic activity in the presence of increasing concentrations of LSD and mescaline. The results obtained led to the conclusion that there is an effect exerted by both compounds on the mitochondrial activity and, eventually, on the glycolytic flux of the studied yeast. This study shows, for the first time, an increase in cellular metabolism in the presence of LSD and mescaline. This observation may be associated with the improvements detected clinically. This is a pioneering and exploratory study, so it will be necessary to repeat some tests to confirm the conclusions presented.
The use of psychedelic compounds in ritual and religious contexts is multicultural and has prehistoric origins. In ancient human societies, these substances were used to alter the state of consciousness of shamans, putting them “in touch with divinity”. These substances were banned during Richard Nixon’s administration in the mid-1970s, resulting in their stigmatization. Towards the end of the 1980s, the use of these compounds in a clinical context began to revive, with a more marked increase in recent years. This study aims to contribute to understanding the cellular and molecular mechanisms of mescaline and LSD using a cellular model of Saccharomyces cerevisiae yeast. This yeast has similar molecular and cellular mechanisms to human cells, making this a simpler approach to understanding the fundamental biochemistry of cells under different conditions. The methodologies used made it possible to evaluate the parameters associated with cell growth, as well as cell sensitivity, viability, and metabolic activity in the presence of increasing concentrations of LSD and mescaline. The results obtained led to the conclusion that there is an effect exerted by both compounds on the mitochondrial activity and, eventually, on the glycolytic flux of the studied yeast. This study shows, for the first time, an increase in cellular metabolism in the presence of LSD and mescaline. This observation may be associated with the improvements detected clinically. This is a pioneering and exploratory study, so it will be necessary to repeat some tests to confirm the conclusions presented.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Ensaios de toxicidade LSD Mescalina Saccharomyces cerevisiae