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Detection and quantification of selected cannabinoids in oral fluid samples by protein precipitation and LC-MS/MS

2024Journal article Open access
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dc.contributor.authorAntunes, Mónica
dc.contributor.authorSimões, Susana
dc.contributor.authorFonseca, Suzana
dc.contributor.authorFranco, João
dc.contributor.authorGallardo, Eugenia
dc.contributor.authorBarroso, Mário
dc.date.accessioned2024-09-09T13:46:58Z
dc.date.available2024-09-09T13:46:58Z
dc.date.issued2024
dc.description.abstractCannabis is the most widely consumed illicit drug worldwide. As consumption rates increase, partially due to the decriminalization of its use for medicinal and recreational purposes, analytical methods for monitoring different cannabinoids in several biological matrices have been developed. Herein, a simple and fast extraction procedure to extract natural cannabinoids from oral fluid (OF) samples was developed and fully validated according to the ANSI/ASB 2019 Standard Practices for Method Validation in Forensic Toxicology. Using only 0.2 mL of neat OF, the analytes [Δ9-tetrahidrocannabinol (THC), 11-hydroxy-Δ9-tetrahydrocannabinol (THC-OH), 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH), cannabinol (CBN) and cannabidiol (CBD)] were extracted by protein precipitation with a mixture of methanol:acetonitrile (80:20, v/v); the extracts were centrifuged, evaporated to dryness and reconstituted in 100 µL of methanol. Analysis was performed by liquid chromatography coupled to tandem mass spectrometry (LC–MS/MS). The developed methodology produced linear results for all compounds, with working ranges of 0.1–50 ng/mL for THC, 0.5–50 ng/mL for THC-OH, CBN and CBD, and 0.05–1 ng/mL for THC-COOH. Ion suppression was observed for THC, CBN and CBD, which did not impair sensitivity considering the low limits of quantification (LOQs) and limits of detection (LODs) obtained (which varied between 0.05 and 0.5 ng/mL). The extraction procedure produced great recoveries, and the compounds were stable. No interferences were found, and the method proved to be extremely fast, selective, precise, and accurate for use in routine analysis. The method was successfully applied to authentic samples.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAntunes, M., Simões, S., Fonseca, S., Franco, J., Gallardo, E., & Barroso, M. (2024). Detection and quantification of selected cannabinoids in oral fluid samples by protein precipitation and LC-MS/MS. Forensic science international, 363, 112174pt_PT
dc.identifier.doi10.1016/j.forsciint.2024.112174pt_PT
dc.identifier.issn1872-6283
dc.identifier.urihttp://hdl.handle.net/10400.26/52021
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCannabis Consumption in the University Population in Portugal: Determination of Biomarkers in Hair and Oral Fluid Samples Consumo de Cannabis na População Universitária em Portugal: Determinação de Biomarcadores em Amostras de Cabelo e Saliva
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCannabinoidspt_PT
dc.subjectForensic Toxicologypt_PT
dc.subjectLiquid Chromatography-Mass Spectrometrypt_PT
dc.subjectSubstance Abuse Detectionpt_PT
dc.subjectOral fluidpt_PT
dc.subjectProtein precipitationpt_PT
dc.titleDetection and quantification of selected cannabinoids in oral fluid samples by protein precipitation and LC-MS/MSpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCannabis Consumption in the University Population in Portugal: Determination of Biomarkers in Hair and Oral Fluid Samples Consumo de Cannabis na População Universitária em Portugal: Determinação de Biomarcadores em Amostras de Cabelo e Saliva
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/2020.05765.BD/PT
oaire.citation.startPage112174pt_PT
oaire.citation.titleForensic Science Internationalpt_PT
oaire.citation.volume363pt_PT
oaire.fundingStreamPOR_CENTRO
person.familyNameSimões
person.familyNameFonseca
person.familyNameFranco
person.familyNameBarroso
person.givenNameSusana
person.givenNameSuzana
person.givenNameJoão
person.givenNameMário
person.identifier.ciencia-idC31D-C5F1-D6BD
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.orcid0000-0002-9457-1546
person.identifier.orcid0000-0001-9122-1017
person.identifier.orcid0000-0002-3268-3841
person.identifier.orcid0000-0001-8848-2734
person.identifier.scopus-author-id35329243100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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