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Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi

2022-05Contribution to journal Open access
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datacite.subject.fosCiências Médicas
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorPlácido, Alexandra
dc.contributor.authorAmaral, Constança do Pais do
dc.contributor.authorTeixeira, Cátia
dc.contributor.authorNogueira, Ariane
dc.contributor.authorBrango-Vanegas, José
dc.contributor.authorBarbosa, Eder Alves
dc.contributor.authorMoreira, Daniel C.
dc.contributor.authorSilva-Carvalho, Amanda É.
dc.contributor.authorSilva, Maria da Gloria da
dc.contributor.authorDias, Jhones do Nascimento
dc.contributor.authorAlbuquerque, Patrícia
dc.contributor.authorSaldanha-Araújo, Felipe
dc.contributor.authorLima, Filipe C. D. A.
dc.contributor.authorBatagin-Neto, Augusto
dc.contributor.authorKuckelhaus, Selma
dc.contributor.authorBessa, Lucinda J.
dc.contributor.authorFreitas, Jaime
dc.contributor.authorBrand, Guilherme Dotto
dc.contributor.authorSantos, Nuno C.
dc.contributor.authorRelvas, João B.
dc.contributor.authorGomes, Paula
dc.contributor.authorLeite, José Roberto S. A.
dc.contributor.authorEaton, Peter
dc.date.accessioned2025-11-21T11:54:21Z
dc.date.available2025-11-21T11:54:21Z
dc.date.issued2022-05
dc.description.abstractTryptophyllins constitute a heterogeneous group of peptides that are one of the first classes of peptides identified from amphibian’s skin secretions. Here, we report the structural characterization and antioxidant properties of a novel tryptophyllin-like peptide, named PpT-2, isolated from the Iberian green frog Pelophylax perezi. The skin secretion of P. perezi was obtained by electrical stimulation and fractionated using RP-HPLC. De novo peptide sequencing was conducted using MALDI MS/MS. The primary structure of PpT-2 (FPWLLS-NH2) was confirmed by Edman degradation and subsequently investigated using in silico tools. PpT-2 shared physicochemical properties with other well-known antioxidants. To test PpT-2 for antioxidant activity in vitro, the peptide was synthesized by solid phase and assessed in the chemical-based ABTS and DPPH scavenging assays. Then, a flow cytometry experiment was conducted to assess PpT-2 antioxidant activity in oxidatively challenged murine microglial cells. As predicted by the in silico analyses, PpT-2 scavenged free radicals in vitro and suppressed the generation of reactive species in PMA-stimulated BV-2 microglia cells. We further explored possible bioactivities of PpT-2 against prostate cancer cells and bacteria, against which the peptide exerted a moderate antiproliferative effect and negligible antimicrobial activity. The biocompatibility of PpT-2 was evaluated in cytotoxicity assays and in vivo toxicity with Galleria mellonella. No toxicity was detected in cells treated with up to 512 µg/ml and in G. mellonella treated with up to 40 mg/kg PpT-2. This novel peptide, PpT-2, stands as a promising peptide with potential therapeutic and biotechnological applications, mainly for the treatment/prevention of neurodegenerative disorders.eng
dc.identifier.citationPlácido A, Pais do Amaral C, Teixeira C, et al. Neuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezi. J Cell Mol Med. 2022; 26: 2793–2807. doi:10.1111/jcmm.17292
dc.identifier.doi10.1111/jcmm.17292
dc.identifier.issn1582-4934
dc.identifier.urihttp://hdl.handle.net/10400.26/59927
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relation.hasversionhttps://doi.org/10.1111/jcmm.17292
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectAmphibia
dc.subjectantioxidant
dc.subjectbioactive peptide
dc.subjectneuroprotection
dc.subjectPelophylax perezi
dc.subjecttryptophyllin
dc.titleNeuroprotective effects on microglia and insights into the structure–activity relationship of an antioxidant peptide isolated from Pelophylax perezieng
dc.typecontribution to journal
dspace.entity.typePublication
oaire.citation.endPage2807
oaire.citation.issue10
oaire.citation.startPage2793
oaire.citation.titleJournal of Cellular and Molecular Medicine
oaire.citation.volume26
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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