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The potential of dried saliva spots for antidepressant drugs monitoring

2023-10Conference object Open access
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dc.contributor.authorSoares, Sofia
dc.contributor.authorRosado, Tiago
dc.contributor.authorBarroso, Mário
dc.contributor.authorGallardo, Eugenia
dc.date.accessioned2024-01-25T10:25:13Z
dc.date.available2024-01-25T10:25:13Z
dc.date.issued2023-10
dc.descriptionComunicação apresentada no 10º Congresso Iberoamericano de Ciências Farmacêuticas, Coimbra, Outubro de 2023. Abstract da comunicação publicado na Acta Farmacêutica Portuguesa. vol. de Outubro 2023. ISSN 2182-3340.pt_PT
dc.description.abstractIntroduction: Portugal has one of the highest rates of mental illness in Europe, and the consumption of antidepressants amongst its population has doubled between the years 2013 and 2016. Approximately 30 million packs of medication are prescribed and dispensed annually in this country to treat major depressive disorders. Therapeutic Drug Monitoring (TDM) is a practice established for a small number of drugs, for which there is a direct relationship between concentration and pharmacological effect at the site of action. Commonly, TDM involves measuring drug concentrations in serum, plasma or blood, being complemented with clinical observation measures to assess patient’s condition, support the individualization of therapy, detect changes in the patient´s pathophysiological status or the modification of the drug pharmacokinetics, but also to assess adherence to therapy. Methodology: The present work describes a methodology with great potential for TDM purposes, allowing the determination of antidepressants (fluoxetine, venlafaxine, citalopram, sertraline, paroxetine and metabolites) using 100 μL of oral fluid with dried saliva spots (DSS) and analysis by gas chromatography-tandem mass spectrometry (GC-MS/MS). Results: For both sampling and extraction with DSS, several parameters were optimized using the statistical tool Design of Experiments (DoE), namely: drying and extraction times, extraction solvent and volume. The final optimized conditions were: 1 hour of drying, 1 mL of methanol and 5 minutes of extraction. The method was validated showing linearity within the adopted therapeutic ranges, with limits of detection and quantification between 10 and 100 ng/mL. All validation parameters were assayed according to the SWGTOX guidelines. Discussion: This is the first work that uses DSS to extract antidepressants from oral fluid samples, proving to be a sensitive, simple and fast alternative to conventional techniques and samples used in TDM (solid phase extraction based procedures and plasma samples). It also reveals potential to be routinely applied in TDM and forensic toxicology purposes, as it has been successfully applied to the analysis of authentic patient samples.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/49349
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.relationThe potential use of oral fluid to monitor antidepressants in clinical and forensic scenarios
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAntidepressantspt_PT
dc.subjectDSSpt_PT
dc.subjectDrug Monitoringpt_PT
dc.titleThe potential of dried saliva spots for antidepressant drugs monitoringpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleThe potential use of oral fluid to monitor antidepressants in clinical and forensic scenarios
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F148753%2F2019/PT
oaire.citation.conferencePlaceCoimbrapt_PT
oaire.citation.titleX Congresso Iberoamericano de Ciências Farmacêuticas, Coimbra, outubro de 2023pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBarroso
person.givenNameMário
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.orcid0000-0001-8848-2734
person.identifier.scopus-author-id35329243100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication294b555e-48c4-4013-b981-b8917400c56c
relation.isAuthorOfPublication.latestForDiscovery294b555e-48c4-4013-b981-b8917400c56c
relation.isProjectOfPublication4ca3c3c9-1802-4c7f-8730-9547236896fe
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