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Estudo dos períodos de pré-colonização e oviposição de dípteros colonizadores primários de cadáveres durante a noite e o dia
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Abstract(s)
Na presente dissertação, desenvolveu-se um estudo sobre a pré-colonização e oviposição de dípteros primários de cadáveres durante o período diurno e noturno, realizado inicialmente na Faculdade de Ciências de Lisboa (FCUL) e posteriormente no Instituto Universitário Egas Moniz (IUEM), entre dezembro e janeiro de 2019, e entre julho e setembro de 2020, respetivamente. Na FCUL realizou-se um ensaio preliminar que visou coletar dípteros colonizadores primários atraídos para armadilhas iscadas com fígado de porco, para
proceder à identificação das espécies mais abundantes na região da Grande Lisboa que devido à pandemia Covid19 apenas decorreu no Outono. No IUEM foram realizados seis ensaios para estudar a pré-colonização
e oviposição de dípteros colonizadores primários que englobaram os períodos diurnos e noturnos, a diferentes temperaturas. Nestes ensaios, os dados meteorológicos e os registos fotográficos foram realizados com o auxílio do aparelho meteorológico Weather Station PCEFWS 20 e a câmara Wingscapes® TimelapseCam Pro WCT00126, respetivamente.
Os espécimes foram recolhidos, identificados e preservados em álcool a 70%. Não foi registada atividade noturna por parte dos dípteros colonizadores primários, mas apenas de uma família de dípteros não identificada, quando a temperatura média noturna foi de 26°C entre as 22h de 16 de julho de 2020 e as 7h de dia 17 de julho de 2020. Durante os estudos diurnos, verificou-se a presença maioritária da espécie
Lucilia sericata (Meigen) nos ensaios realizados entre julho e setembro de 2020. A análise de dados mostrou uma maior probabilidade de oviposição nas temperaturas mais elevadas. A precipitação e a humidade elevadas mostraram ter um impacto negativo na realização de oviposição, bem como no desenvolvimento dos ovos, sendo por isso fatores a ter em conta na determinação do intervalo post mortem (IPM).
In this thesis a study has been developed about precolonization and oviposition of primary diptera with forensic importance at daytime and nocturnal time performed initially at Faculty of Sciences of the University of Lisbon (FCUL) and later at the University Institute of Egas Moniz (IUEM) between December and January of 2019, and July and September of 2020, respectively. At FCUL a preliminary test was performed which aimed to collect primary colonizing diptera attracted to baited traps with pig liver, to proceed to the identification of the most abundant species in the Great Lisbon, that due to the Covid19 pandemic only took place in autumn. In IUEM six experiments were performed to study the precolonization and oviposition of primary colonizing diptera at diurnal and nocturnal time at different temperatures. In these tests the meteorological data and photographic records were performed with the assistance of meteorological device Weather Station PCEFWS 20 and Wingscapes® TimelapseCam Pro WCT00126 camera, respectively. The specimens were collected, identified, and preserved in 70% alcohol. No nocturnal activity was recorded by the primary colonizing diptera, but by an unidentified family when the average nocturnal temperature was 26°C (79°F) between 10pm of July 16th and 7am of July 17th of 2020. During daytime studies, it was found the majority presence of the species Lucilia sericata (Meigen) in tests carried out between July and September of 2020. Data analysis showed a higher probability of oviposition at high temperatures. High rainfall and humidity shown to have a negative impact on oviposition as well as on egg development, which is why these factors have to be considered in determining the post mortem interval (PMI).
In this thesis a study has been developed about precolonization and oviposition of primary diptera with forensic importance at daytime and nocturnal time performed initially at Faculty of Sciences of the University of Lisbon (FCUL) and later at the University Institute of Egas Moniz (IUEM) between December and January of 2019, and July and September of 2020, respectively. At FCUL a preliminary test was performed which aimed to collect primary colonizing diptera attracted to baited traps with pig liver, to proceed to the identification of the most abundant species in the Great Lisbon, that due to the Covid19 pandemic only took place in autumn. In IUEM six experiments were performed to study the precolonization and oviposition of primary colonizing diptera at diurnal and nocturnal time at different temperatures. In these tests the meteorological data and photographic records were performed with the assistance of meteorological device Weather Station PCEFWS 20 and Wingscapes® TimelapseCam Pro WCT00126 camera, respectively. The specimens were collected, identified, and preserved in 70% alcohol. No nocturnal activity was recorded by the primary colonizing diptera, but by an unidentified family when the average nocturnal temperature was 26°C (79°F) between 10pm of July 16th and 7am of July 17th of 2020. During daytime studies, it was found the majority presence of the species Lucilia sericata (Meigen) in tests carried out between July and September of 2020. Data analysis showed a higher probability of oviposition at high temperatures. High rainfall and humidity shown to have a negative impact on oviposition as well as on egg development, which is why these factors have to be considered in determining the post mortem interval (PMI).
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Entomologia forense Oviposição noturna Oviposição diurna Intervalo de pré-aparecimento