Repository logo
 
Publication

HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola

2014-12-05Journal article Open access
Simple item page
dc.contributor.authorBártolo, Inês
dc.contributor.authorZakovic, Susana
dc.contributor.authorMartin, Francisco
dc.contributor.authorPalladino, Claudia
dc.contributor.authorCarvalho, Patrícia
dc.contributor.authorCamacho, Ricardo
dc.contributor.authorThamm, Sven
dc.contributor.authorClemente, Sofia
dc.contributor.authorTaveira, Nuno
dc.date.accessioned2015-04-08T14:10:44Z
dc.date.available2015-04-08T14:10:44Z
dc.date.issued2014-12-05
dc.descriptionThis is an openaccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.por
dc.description.abstract"Objectives: To assess HIV-1 diversity, transmission dynamics and prevalence of transmitted drug resistance (TDR) in Angola, five years after ART scale-up. Methods: Population sequencing of the pol gene was performed on 139 plasma samples collected in 2009 from drug-naive HIV-1 infected individuals living in Luanda. HIV-1 subtypes were determined using phylogenetic analysis. Drug resistance mutations were identified using the Calibrated Population Resistance Tool (CPR). Transmission networks were determined using phylogenetic analysis of all Angolan sequences present in the databases. Evolutionary trends were determined by comparison with a similar survey performed in 2001. Results: 47.1% of the viruses were pure subtypes (all except B), 47.1% were recombinants and 5.8% were untypable. The prevalence of subtype A decreased significantly from 2001 to 2009 (40.0% to 10.8%, P50.0019) while the prevalence of unique recombinant forms (URFs) increased.2-fold (40.0% to 83.1%, P,0.0001). The most frequent URFs comprised untypable sequences with subtypes H (U/H, n57, 10.8%), A (U/A, n56, 9.2%) and G (G/U, n54, 6.2%). Newly identified U/H recombinants formed a highly supported monophyletic cluster suggesting a local and common origin. TDR mutation K103N was found in one (0.7%) patient (1.6% in 2001). Out of the 364 sequences sampled for transmission network analysis, 130 (35.7%) were part of a transmission network. Forty eight transmission clusters were identified; the majority (56.3%) comprised sequences sampled in 2008–2010 in Luanda which is consistent with a locally fuelled epidemic. Very low genetic distance was found in 27 transmission pairs sampled in the same year, suggesting recent transmission events. Conclusions: Transmission of drug resistant strains was still negligible in Luanda in 2009, five years after the scale-up of ART. The dominance of small and recent transmission clusters and the emergence of new URFs are consistent with a rising HIV-1 epidemics mainly driven by heterosexual transmission."
dc.description.sponsorshipThis work was supported by Fundação para a Ciência e Tecnologia (FCT) http://www.fct. pt), Portugal, (grants ‘‘PTDC/SAU-FAR/115290/ 2009’’ and ‘‘PTDC/SAU-EPI/122400/2010’’), and by Collaborative HIV and Anti-HIV Drug Resistance Network (CHAIN), from the European Union. Inês Bártolo was supported by a post-doc fellowship (SFRH/BPD/76225/2011) from Fundação para a Ciência e Tecnologia (FCT), Portugal. Suzana Zakovic was supported by a European Union studentship from the Erasmus Mundus Masters in Forensic Sciences (http://forensicerasmusmundus. blogs.lincoln.ac.uk/). Francisco Martin was supported by a PhD fellowship (SFRH/BD/87488/2012) from Fundação para a Ciência e Tecnologia (FCT), Portugal. Claudia Palladino was supported by a post-doc fellowship (SFRH/BPD/77448/2011) from Fundação para a Ciência e Tecnologia (FCT), Portugal. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.por
dc.identifier.citationBártolo I, Zakovic S, Martin F, Palladino C, Carvalho P, et al. (2014) HIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angola. PLoS ONE 9(12): e113626. doi:10.1371/journal.pone.0113626por
dc.identifier.doi10.1371/journal.pone.0113626
dc.identifier.issn1932-6203
dc.identifier.urihttp://hdl.handle.net/10400.26/8231
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherPLOSpor
dc.relationMolecular epidemiology, drug resistance and pathogenesis of HIV and TB in Angola: the Angolan PErinatal HIV Cohort (APEHC)
dc.relationDESENVOLVIMENTO DE NOVOS MICROBICIDAS PARA PREVENIR A INFECÇÃO POR VIH
dc.relation.publisherversionhttp://journals.plos.org/plosone/article?id=10.1371/journal.pone.0113626por
dc.subjectHIV-1por
dc.subjectAngolapor
dc.titleHIV-1 Diversity, Transmission Dynamics and Primary Drug Resistance in Angolapor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleMolecular epidemiology, drug resistance and pathogenesis of HIV and TB in Angola: the Angolan PErinatal HIV Cohort (APEHC)
oaire.awardTitleDESENVOLVIMENTO DE NOVOS MICROBICIDAS PARA PREVENIR A INFECÇÃO POR VIH
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-EPI%2F122400%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F77448%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-FAR%2F115290%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F76225%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F87488%2F2012/PT
oaire.citation.startPagee113626por
oaire.citation.titlePLoS ONEpor
oaire.citation.volume9(12)por
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
oaire.fundingStream3599-PPCDT
oaire.fundingStreamSFRH
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isProjectOfPublicationc3b60260-41c1-4212-93cd-191d5b2e6133
relation.isProjectOfPublication6820802c-e181-4622-be67-b4e7af9d2ba1
relation.isProjectOfPublication15aded3d-70a1-4e02-8508-f5e6a4e56f9a
relation.isProjectOfPublicationc548f6e8-c9c8-4ad9-9572-d64134c3a608
relation.isProjectOfPublicationa39e07a2-cbb9-4cf3-9fc2-354373f654f8
relation.isProjectOfPublication.latestForDiscoveryc3b60260-41c1-4212-93cd-191d5b2e6133

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Art01_2015ProfNTaveira.pdf
Size:
948.72 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.85 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

EM - Artigos Científicos