dc.contributor.author	Rosengart, Alessandro
dc.contributor.author	Cesário, M.Teresa
dc.contributor.author	Almeida, M. Catarina M.D. de
dc.contributor.author	Raposo, Rodrigo S.
dc.contributor.author	Espert, Ana
dc.contributor.author	Díaz de Apodaca, Elena
dc.contributor.author	Fonseca, M. Manuela R. da
dc.date.accessioned	2016-09-21T11:58:09Z
dc.date.available	2016-10-31T01:30:11Z
dc.date.issued	2015-11
dc.description	© 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/	pt_PT
dc.description.abstract	"A technique using safer, non-chlorinated organic solvents for the extraction of poly-3-hydroxybutyrate (P(3HB)) from bacterial cells was developed, aiming to attain high recovery yields and purities. Some solvents were selected from the GlaxoSmithKline guide as sustainable industrial solvents and the solubility of P(3HB) calculated using predictive equations from literature. Based on the calculated solubility values, anisole, cyclohexanone and phenetole were tested as extraction solvents and the relevant process variables (extraction temperature, extraction time and mass of cells/solvent volume ratio) were addressed. Polymer recovery yields of 97% and 93% were obtained with anisole and cyclohexanone, respectively, at 120–130 °C using a cell/solvent ratio of 1.5% (w/v). Maximum polymer purities using these experimental conditions were 98% for both solvents. The recovery yield and the polymer purity attained with chloroform (reference solvent) were 96 and 98%, respectively. Higher cell/solvent ratios of 6.0% (w/v) showed slightly lower recovery yields and purities. The average molecular weight and the thermal properties of the polymers extracted with the alternative solvents were fully comparable to those of the polymers obtained by chloroform extraction, demonstrating that the applied conditions did not significantly alter the properties of the extracted P(3HB)."	pt_PT
dc.identifier.citation	Biochemical Engineering Journal Volume 103, 15 November 2015, Pages 39–46	pt_PT
dc.identifier.doi	10.1016/j.bej.2015.06.013	pt_PT
dc.identifier.issn	1369-703X
dc.identifier.uri	http://hdl.handle.net/10400.26/14802
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.publisher	Elsevier	pt_PT
dc.relation	New tailor-made PHB-based nanocomposites for high performance applications produced from environmentally friendly production routes
dc.relation	PRODUCTION OF POLY 3-HYDROXYBUTYRATE-CO- 4-HYDROXYBUTYRATE P 3HB-CO-4HB FROM LIGNOCELLULOSIC AGRO-INDUSTRIAL WASTES
dc.relation	PRODUÇÃO DE POLIHIDROXIALCANOATOS UTILIZANDO SUBSTRATOS RESIDUAIS
dc.relation.publisherversion	http://dx.doi.org/10.1016/j.bej.2015.06.013	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by-nc-nd/4.0/	pt_PT
dc.subject	Downstream processing	pt_PT
dc.subject	Cell disruption	pt_PT
dc.subject	Separation	pt_PT
dc.subject	Purification	pt_PT
dc.subject	Poly(3-hydroxybutyrate) recovery	pt_PT
dc.subject	Anisole	pt_PT
dc.subject	Cyclohexanone	pt_PT
dc.title	Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents	pt_PT
dc.type	journal article
dspace.entity.type	Publication
oaire.awardTitle	New tailor-made PHB-based nanocomposites for high performance applications produced from environmentally friendly production routes
oaire.awardTitle	PRODUCTION OF POLY 3-HYDROXYBUTYRATE-CO- 4-HYDROXYBUTYRATE P 3HB-CO-4HB FROM LIGNOCELLULOSIC AGRO-INDUSTRIAL WASTES
oaire.awardTitle	PRODUÇÃO DE POLIHIDROXIALCANOATOS UTILIZANDO SUBSTRATOS RESIDUAIS
oaire.awardURI	info:eu-repo/grantAgreement/EC/FP7/246449/EU
oaire.awardURI	info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F68587%2F2010/PT
oaire.awardURI	info:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F26678%2F2006/PT
oaire.citation.endPage	46	pt_PT
oaire.citation.startPage	39	pt_PT
oaire.citation.title	Biochemical Engineering Journal	pt_PT
oaire.citation.volume	103(15)	pt_PT
oaire.fundingStream	FP7
project.funder.identifier	http://doi.org/10.13039/501100008530
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.identifier	http://doi.org/10.13039/501100001871
project.funder.name	European Commission
project.funder.name	Fundação para a Ciência e a Tecnologia
project.funder.name	Fundação para a Ciência e a Tecnologia
rcaap.rights	openAccess	pt_PT
rcaap.type	article	pt_PT
relation.isProjectOfPublication	e88d1819-1f6d-4b1d-a864-ec2091b17e8a
relation.isProjectOfPublication	8b03dfca-bdb8-448e-8333-cbd672ab33ee
relation.isProjectOfPublication	54143930-0087-45be-ae76-b932ef0f1af1
relation.isProjectOfPublication.latestForDiscovery	e88d1819-1f6d-4b1d-a864-ec2091b17e8a

Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents

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