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Stepwise functional evolution in a fungal sugar transporter family

2016-02Journal article Open access
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dc.contributor.authorGonçalves, Carla
dc.contributor.authorCoelho, Mauro A.
dc.contributor.authorSalema-Oom, Madalena
dc.contributor.authorGonçalves, Paula
dc.date.accessioned2017-01-18T09:16:31Z
dc.date.available2017-03-01T01:30:10Z
dc.date.issued2016-02
dc.descriptionThis is a pre-copyedited, author-produced version of an article accepted for publication in Molecular Biology and Evolution following peer review. The version of record Mol Biol Evol (2016) 33 (2): 352-366 is available online at: http://mbe.oxfordjournals.org/content/33/2/352 DOI 10.1093/molbev/msv220.pt_PT
dc.description.abstract"Sugar transport is of the utmost importance for most cells and is important to a wide range of applied fields. However, despite the straightforward in silico assignment of many novel transporters, including sugar porters, to existing families, their exact biological role and evolutionary trajectory often remain unclear, mainly because biochemical characterization of membrane proteins is inherently challenging, but also owing to their uncommonly turbulent evolutionary histories. In addition, many important shifts in membrane carrier function are apparently ancient, which further limits our ability to reconstruct evolutionary trajectories in a reliable manner.pt_PT
dc.description.abstractHere we circumvented some of these obstacles by examining the relatively recent emergence of a unique family of fungal sugar facilitators, related to drug antiporters. The former transporters, named Ffz, were previously shown to be required for fructophilic metabolism in yeasts. We first exploited the wealth of fungal genomic data available to define a comprehensive but well-delimited family of Ffz-like transporters, showing that they are only present in Dikarya. Subsequently, a combination of phylogenetic analyses and in vivo functional characterization was used to retrace important changes in function, while highlighting the evolutionary events that are most likely to have determined extant distribution of the gene, such as horizontal gene transfers (HGTs). One such HGT event is proposed to have set the stage for the onset of fructophilic metabolism in yeasts, a trait that according to our results may be the metabolic hallmark of approximately one hundred yeast species that thrive in sugar rich environments."pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCarla Gonçalves, Marco A. Coelho, Madalena Salema-Oom, and Paula Gonçalves. Stepwise Functional Evolution in a Fungal Sugar Transporter Family. Mol Biol Evol (2016) 33 (2): 352-366 first published online October 15, 2015. doi:10.1093/molbev/msv220pt_PT
dc.identifier.doi10.1093/molbev/msv220pt_PT
dc.identifier.issn1537-1719
dc.identifier.issn0737-4038
dc.identifier.urihttp://hdl.handle.net/10400.26/17556
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relationImprovement of fructose fermentation by industrial Saccharomyces cerevisiae strains
dc.relation.publisherversionhttp://mbe.oxfordjournals.org/content/33/2/352pt_PT
dc.subjectFructose transportpt_PT
dc.subjectYeast metabolic evolutionpt_PT
dc.subjectHorizontal gene transferpt_PT
dc.subjectFfzpt_PT
dc.subjectDHA1pt_PT
dc.titleStepwise functional evolution in a fungal sugar transporter familypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleImprovement of fructose fermentation by industrial Saccharomyces cerevisiae strains
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FAGR-ALI%2F112802%2F2009/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04378%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F89489%2F2012/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F79198%2F2011/PT
oaire.citation.endPage366pt_PT
oaire.citation.startPage352pt_PT
oaire.citation.titleMolecular Biology and Evolutionpt_PT
oaire.citation.volume33(2)pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream5876
oaire.fundingStreamSFRH
oaire.fundingStreamSFRH
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationf30c892b-3cc0-460d-b83d-b0292a8dea38
relation.isProjectOfPublication97c6e39e-a353-4d90-aa2c-bef5fdf75e59
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relation.isProjectOfPublication4b926945-c3e5-4914-bce7-3e7f66a2887d
relation.isProjectOfPublication.latestForDiscoveryf30c892b-3cc0-460d-b83d-b0292a8dea38

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