Publication
A new pathway for Mannitol metabolism in yeasts suggests a link to the evolution of alcoholic fermentation
| dc.contributor.author | Gonçalves, Carla | |
| dc.contributor.author | Ferreira, Carolina | |
| dc.contributor.author | Gonçalves, Luís G. | |
| dc.contributor.author | Turner, David L. | |
| dc.contributor.author | Leandro, Maria José | |
| dc.contributor.author | Salema-Oom, Madalena | |
| dc.contributor.author | Santos, Helena | |
| dc.contributor.author | Gonçalves, Paula | |
| dc.date.accessioned | 2019-12-16T16:20:07Z | |
| dc.date.available | 2019-12-16T16:20:07Z | |
| dc.date.issued | 2019-11 | |
| dc.description | This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | pt_PT |
| dc.description.abstract | The yeasts belonging to the Wickerhamiella and Starmerella genera (W/S clade) share a distinctive evolutionary history marked by loss and subsequent reinstatement of alcoholic fermentation mediated by horizontal gene transfer events. Species in this clade also share unusual features of metabolism, namely the preference for fructose over glucose as carbon source, a rare trait known as fructophily. Here we show that fructose may be the preferred sugar in W/S-clade species because, unlike glucose, it can be converted directly to mannitol in a reaction with impact on redox balance. According to our results, mannitol is excreted to the growth medium in appreciable amounts along with other fermentation products such as glycerol and ethanol but unlike the latter metabolites mannitol production increases with temperature. We used comparative genomics to find genes involved in mannitol metabolism and established the mannitol biosynthesis pathway in W/S-clade species Starmerella bombicola using molecular genetics tools. Surprisingly, mannitol production seems to be so important that St. bombicola (and other W/S-clade species) deploys a novel pathway to mediate the conversion of glucose to fructose, thereby allowing cells to produce mannitol even when glucose is the sole carbon source. Using targeted mutations and 13C-labeled glucose followed by NMR analysis of end-products, we showed that the novel mannitol biosynthesis pathway involves fructose-6-phosphate as an intermediate, implying a key role for a yet unknown fructose-6-P phosphatase. We hypothesize that mannitol production contributed to mitigate the negative effects on redox balance of the ancient loss of alcoholic fermentation in the W/S clade. Presently, mannitol also seems to play a role in stress protection. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Gonçalves C, Ferreira C, Gonçalves LG, Turner DL, Leandro MJ, Salema-Oom M, Santos H and Gonçalves P (2019) A New Pathway for Mannitol Metabolism in Yeasts Suggests a Link to the Evolution of Alcoholic Fermentation. Front. Microbiol. 10:2510. doi: 10.3389/fmicb.2019.02510 | pt_PT |
| dc.identifier.doi | 10.3389/fmicb.2019.02510 | pt_PT |
| dc.identifier.issn | 1664-302X | |
| dc.identifier.uri | http://hdl.handle.net/10400.26/30511 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Frontiers Media | pt_PT |
| dc.relation.publisherversion | https://doi.org/10.3389/fmicb.2019.02510 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | pt_PT |
| dc.subject | Alcoholic fermentation | pt_PT |
| dc.subject | Yeast metabolism | pt_PT |
| dc.subject | Mannitol metabolism | pt_PT |
| dc.subject | Starmerella | pt_PT |
| dc.subject | Fructophily | pt_PT |
| dc.title | A new pathway for Mannitol metabolism in yeasts suggests a link to the evolution of alcoholic fermentation | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F89489%2F2012/PT | |
| oaire.citation.startPage | 2510 | pt_PT |
| oaire.citation.title | Frontiers in Microbiology | pt_PT |
| oaire.citation.volume | 10 | pt_PT |
| oaire.fundingStream | SFRH | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | openAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | d08d624b-5067-44c9-8311-735c752ee1d2 | |
| relation.isProjectOfPublication.latestForDiscovery | d08d624b-5067-44c9-8311-735c752ee1d2 |