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Stability of Cocaine, Opiates, and Metabolites in Dried Saliva Spots

2022Journal article Open access
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dc.contributor.authorAlmeida, Ema
dc.contributor.authorSoares, Sofia
dc.contributor.authorGonçalves, Joana
dc.contributor.authorRosado, Tiago
dc.contributor.authorFernández, Nicolás
dc.contributor.authorRodilla, Jesus M.
dc.contributor.authorPassarinha, Luís A.
dc.contributor.authorBarroso, Mário
dc.contributor.authorGallardo, Eugenia
dc.date.accessioned2022-07-04T15:17:07Z
dc.date.available2022-07-04T15:17:07Z
dc.date.issued2022
dc.description.abstractDrug abuse still represents a global problem, and it is associated with an increased risk of diseases, injuries, and deaths. Cocaine (COC) and opiates are the most abused drugs and account for a significant number of fatalities. Therefore, it is important to develop methods capable of effectively identifying and quantifying these substances. The present study aims to evaluate the long- term stability of COC, ecgonine methylester (EME), benzoylecgonine (BEG), cocaethylene (COET), norcocaine (NCOC), morphine (MOR), codeine (COD) and 6-monoacetylmorphine (6-MAM) in oral fluid samples. The analytes of interest were isolated from the matrix (50 μL) using the dried saliva spots (DSS) sampling approach and were subsequently analyzed by gas chromatography coupled with tandem mass spectrometry (GC–MS/MS). The parameters that could influence the stability of the target compounds were studied, and these were storage temperature, light, use of preservatives (and respective concentrations), and time. The effects of each parameter were evaluated using the design of experiments (DOE) approach. The stability of the target analytes was improved when the DSS were stored at room temperature, in the presence of light and using 1% sodium fluoride. The best conditions were then adopted for the DSS storage and long-term stability was assessed. COD was only stable for 1 day, EME was stable for 3 days, COC, COET, NCOC and 6-MAM were stable for 7 days, MOR for 14 days and BEG remained stable throughout the study (136 days). This is the first study that evaluates the stability of these compounds in oral fluid samples after application in DSS cards, and optimizes the conditions in order to improve their stabilitypt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAlmeida E, Soares S, Gonçalves J, Rosado T, Fernández N, Rodilla JM, Passarinha LA, Barroso M, Gallardo E. Stability of Cocaine, Opiates, and Metabolites in Dried Saliva Spots. Molecules. 2022; 27(3):641pt_PT
dc.identifier.doi10.3390/molecules27030641pt_PT
dc.identifier.issn1420-3049
dc.identifier.urihttp://hdl.handle.net/10400.26/41268
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationApplied Molecular Biosciences Unit
dc.relationalterado para: “Avaliação in vitro associados ao consumo de Ayahuasca” AVALIAÇÃO IN VITRO DOS EFEITOS TOXICOLÓGICOS ASSOCIADOS AO CONSUMO DE AYAHUASCA.
dc.relationHealth Sciences Research Centre
dc.relationApplied Molecular Biosciences Unit
dc.relationHealth Sciences Research Centre
dc.relation.publisherversionhttps://www.mdpi.com/1420-3049/27/3/641pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCocainept_PT
dc.subjectOpiatespt_PT
dc.subjectOral fluidpt_PT
dc.subjectDried saliva spotspt_PT
dc.titleStability of Cocaine, Opiates, and Metabolites in Dried Saliva Spotspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitlealterado para: “Avaliação in vitro associados ao consumo de Ayahuasca” AVALIAÇÃO IN VITRO DOS EFEITOS TOXICOLÓGICOS ASSOCIADOS AO CONSUMO DE AYAHUASCA.
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleApplied Molecular Biosciences Unit
oaire.awardTitleHealth Sciences Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F149360%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04378%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.citation.conferencePlaceBASEL, SWITZERLANDpt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage641pt_PT
oaire.citation.titleMoleculespt_PT
oaire.citation.volume27pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamPOR_CENTRO
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBarroso
person.givenNameMário
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.orcid0000-0001-8848-2734
person.identifier.scopus-author-id35329243100
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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