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Efeito citotóxico e genotóxico de três resinas compostas Bulk Fill utilizadas em dentisteria
| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 1.45 MB | Adobe PDF |
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Advisor(s)
Abstract(s)
Introdução: As resinas compostas são rotineiramente utilizadas em procedimentos de restauração dentária e podem permancer na cavidade oral por um longo tempo, sendo assim, testes de biocompatibilidade necessários afim de verificar o seu potencial citotóxico/genotóxico. Nos últimos anos, uma nova categoria de resinas compostas Bulk Fill foi desenvolvida para simplificar e agilizar o processo restaurador, podendo ser
aplicadas em camadas de 4-5 mm.
Objectivo: Avaliar a citotoxicidade e a genotocicidade de três resinas Bulk Fill FiltekTM Bulk Fill (R1), Tetric EvoCeram® Bulk Fill (R2) e Coltene Fill-Up!TM (R3).
Métodos: para avaliar a citotoxicidade foi realizado o Ensaio do Cristal Violeta e para a genotoxicidade o Ensaio dos Micronúcleos. Ambos foram realizados in vitro em fibroblastos 3T3 de ratinho. Primeiramente, foi realizado o Teste do Cristal Violeta, através do qual foi calculada a semi-dose letal (DL50) de cada uma das resinas, e posteriormente a DL50 da Resina Coltene Fill-Up foi utilizada no Ensaio dos Micronúcleos.
Resultados: No Ensaio do Cristal Violeta, R3 se mostrou a mais citotóxica, seguida por R2 e R1. Sendo assim, R3 foi a resina utilizada em diferentes concentrações no Ensaio dos Micronúcleos, e em todas elas foram observadas alterações no DNA.
Conclusão: R3 apresentou um baixo grau na conversão dos monómeros e provavelmente por esta razão tem maior toxicidade quando comparada com R1 e R2. Além disso verificámos que a resina Bulk Fill tem capacidade genotóxica.
Introduction: Composite resins are routinely used in dental restoration procedures and can remain in the oral cavity for a long time; thus, biocompatibility tests in order to verify their cytotoxic/genotoxic potential. In recent years, a new category of Bulk Fill composite resins has been developed to simplify and streamline the restorative process, and can be applied in layers of 4-5mm. Ojective: To evaluate cytotoxicity and genotoxicity of three resins Bulk Fill: FiltekTM Bulk Fill (R1), Tetric EvoCeram® (R2) e Coltene Fill-Up! TM (R3). Methods: To evaluate cytotoxicity, the Violet Crystal Assay was performed and for genotoxicity, the Micronucleus Assay. Both were performed in vitro on mouse 3T3 fibroblasts. First, the Violet Crystal Test was performed, through which the semi-lethal dose (LD50) of each resin was calculated, and then LD50 of Coltene Fill-Up Resin was used in the Micronucleus Test. Results: In the Violet Crystal Assay, R3 was the most cytotoxic followed by R2 and R1. Therefore, R3 was the resin used in different concentrations in the Micronucleus Assay, and in all of them alterations in DNA were observed. Conclusions: R3 showed a low degree of conversion of monomers and probably for this reason it has greater toxicity when compared to R1 and R2. In addition, we found that Bulk Fill resin has genotoxic capacity.
Introduction: Composite resins are routinely used in dental restoration procedures and can remain in the oral cavity for a long time; thus, biocompatibility tests in order to verify their cytotoxic/genotoxic potential. In recent years, a new category of Bulk Fill composite resins has been developed to simplify and streamline the restorative process, and can be applied in layers of 4-5mm. Ojective: To evaluate cytotoxicity and genotoxicity of three resins Bulk Fill: FiltekTM Bulk Fill (R1), Tetric EvoCeram® (R2) e Coltene Fill-Up! TM (R3). Methods: To evaluate cytotoxicity, the Violet Crystal Assay was performed and for genotoxicity, the Micronucleus Assay. Both were performed in vitro on mouse 3T3 fibroblasts. First, the Violet Crystal Test was performed, through which the semi-lethal dose (LD50) of each resin was calculated, and then LD50 of Coltene Fill-Up Resin was used in the Micronucleus Test. Results: In the Violet Crystal Assay, R3 was the most cytotoxic followed by R2 and R1. Therefore, R3 was the resin used in different concentrations in the Micronucleus Assay, and in all of them alterations in DNA were observed. Conclusions: R3 showed a low degree of conversion of monomers and probably for this reason it has greater toxicity when compared to R1 and R2. In addition, we found that Bulk Fill resin has genotoxic capacity.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Bulk Fill Ensaio cristal violeta Ensaio dos micronúcleos