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Quantification of Δ9-THC, 11-OH-THC and THCCOOH by SPE and GC-MS-MS in whole blood samples for forensic purposes

2013-09-02Conference object Open access
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dc.contributor.authorCastro, André Lobo
dc.contributor.authorTarelho, Sónia
dc.contributor.authorQuintas, Maria José
dc.contributor.authorCosta, Pedro
dc.contributor.authorMelo, Paula
dc.contributor.authorCastañera, Antonio
dc.contributor.authorDias, Mário
dc.date.accessioned2023-09-05T09:35:23Z
dc.date.available2023-09-05T09:35:23Z
dc.date.issued2013-09-02
dc.descriptionPoster apresentado no 51st Annual Meeting of The International Association of Forensic Toxicologists, Funchal, 2013pt_PT
dc.description.abstractIntroduction: Cannabis sativa represents the most frequently abused illicit drug around the world. Its abuse represents a major issue in terms of public health, not only due to its physical and psychological effects in terms of individual behaviour, but also due to consequences in terms of impairment, mainly as to driving capabilities is concerned. Detection and accurate quantification at low levels, namely for 9-THC and 11-OH-THC, seems crucial, in terms of response of a forensic toxicology laboratory to answer to legal requirements. The aim of this work considers the analytical validation for detection and quantification of 9-THC, 11-OH-THC and THCCOOH in whole blood samples, in vivo and post-mortem, for forensic purposes. Material and methods: The method included an SPE procedure with HLB OASIS® (WATERS) cartridges, derivatization with BSTFA:TMCS 99:1 (SUPELCO) and an hyphenated instrumental technique based in a GC-450 gas chromatograph coupled to a MS-300 triple quadrupole mass detector (BRUKER). Results: The procedure included a chromatographic run, with full separation of the three compounds, and the MS-MS detection was characterized by two product-ions (289 m/z ; 305 m/z), obtained from the parent-ion 371 m/z. The ion 289 was used for quantification. The respective tri-deuterated substances were used as internal standards. The validation included several parameters, as specificity/selectivity (with 0% of False Positives and False Negatives), LOD and LOQ (1 ng/mL to all compounds), linearity (1 – 100 ng/mL to all compounds), extraction recovery, carryover, inter and intra-day run precision (both under 15%), among others. Discussion: The developed method has shown to be fit to purpose, with good results in terms of increased sensibility and detection limits, due to an optimal response from the analytical equipment, based on tandem MS technology. The LOD and LOQ are compatible with reference values for positive real samples, namely applied to traffic roadside testing. This method has been applied to the laboratory routine, representing an effective improvement in terms of the lab response to legal requirements.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/46381
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectTHCpt_PT
dc.subject11-OH-THCpt_PT
dc.subjectTHCCOOHpt_PT
dc.subjectGC-MS/MSpt_PT
dc.titleQuantification of Δ9-THC, 11-OH-THC and THCCOOH by SPE and GC-MS-MS in whole blood samples for forensic purposespt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceFunchal, Portugalpt_PT
oaire.citation.title51st Annual Meeting of The International Association of Forensic Toxicologistspt_PT
person.familyNameLobo Castro
person.familyNameTarelho
person.familyNameLandeiro e Melo
person.familyNameCastañera
person.familyNameDias
person.givenNameAndré
person.givenNameSónia
person.givenNamePaula Isabel
person.givenNameAntonio
person.givenNameMário
person.identifier.ciencia-id2F10-34E1-47B3
person.identifier.orcid0000-0002-7258-8523
person.identifier.orcid0000-0001-8366-6702
person.identifier.orcid0000-0003-0484-0683
person.identifier.orcid0000-0002-3017-8569
person.identifier.orcid0000-0002-5900-5899
person.identifier.scopus-author-id26221673200
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublication631cbcb7-4c75-4fc1-b4cf-8445d428f1de
relation.isAuthorOfPublication0761a9b2-0106-4bdd-876d-6bda23aca720
relation.isAuthorOfPublicationd3578c9c-8367-4088-8551-6b9020896ef7
relation.isAuthorOfPublicationb24227f2-a261-4e33-ab53-3edf34744335
relation.isAuthorOfPublication.latestForDiscoveryd3578c9c-8367-4088-8551-6b9020896ef7

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