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Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates

2014-01Journal article Open access
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dc.contributor.authorCesário, M. Teresa
dc.contributor.authorRaposo, Rodrigo S.
dc.contributor.authorAlmeida, M. Catarina M. D. de
dc.contributor.authorKeulen, Frederik van
dc.contributor.authorFerreira, Bruno S.
dc.contributor.authorFonseca, M. Manuela R. da
dc.date.accessioned2014-05-05T08:32:04Z
dc.date.available2014-05-05T08:32:04Z
dc.date.issued2014-01
dc.descriptionProject: EC/FP7/246449 - New tailor-made PNB-based nanocomposites for high performance applications produced from environmentally friendly production routespor
dc.descriptionNOTICE: this is the author’s version of a work that was accepted for publication in New Biotechnology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in New Biotechnology, [Vol. 31, Issue 1, (January 2014)] DOI 10.1016/j.nbt.2013.10.004
dc.description.abstract"Polyhydroxyalkanoates (PHAs) are bioplastics that can replace conventional petroleum derived products in various applications. One of the major barriers for their widespread introduction in the market is the higher production costs when compared with their petrochemical counterparts. In this work, a process was successfully implemented with high productivity based on wheat straw, a cheap and readily available agricultural residue, as raw material. The strain Burkholderia sacchari DSM 17165 which is able to metabolize glucose, xylose and arabinose, the main sugars present in wheat straw hydrolysates (WSH), was used. Results in shake flask showed that B. sacchari cells accumulated ca 70 % g P(3HB)/g cell-dry-weight with a yield of polymer on sugars (YP/S) of 0.18 g/g when grown on a mixture of commercial C6 and C5 sugars (control), while these values reached ca 60 % g P(3HB)/g cell-dry-weight and 0.19 g/g, respectively, when WSHs were used as carbon source. In fed-batch cultures carried out in 2L stirred tank reactors on WSH, a maximum polymer concentration of 105 g/L was reached after 61 h of cultivation corresponding to an accumulation of 72% of CDW. Polymer yield and productivity were 0.22 g P(3HB)/g total sugar consumed and 1.6 g/L. h, respectively. The selected feeding strategy successfully overcame the carbon catabolite repression phenomenon observed in sugar mixtures containing hexoses and pentoses. This is the first work describing fed-batch cultivations aiming at PHA production using real lignocellulosic hydrolysates. Additionally, the P(3HB) volumetric productivities attained are, by far, the highest achieved ever on agricultural wastes hydrolysates."por
dc.description.sponsorshipUEpor
dc.identifier.citationNew Biotechnology. Volume 31, Issue 1, 25 January 2014, Pages 104–113por
dc.identifier.issn1871-6784
dc.identifier.urihttp://hdl.handle.net/10400.26/6276
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relationPRODUCTION OF POLY 3-HYDROXYBUTYRATE-CO- 4-HYDROXYBUTYRATE P 3HB-CO-4HB FROM LIGNOCELLULOSIC AGRO-INDUSTRIAL WASTES
dc.relationPRODUÇÃO DE POLIHIDROXIALCANOATOS UTILIZANDO SUBSTRATOS RESIDUAIS
dc.relationNew tailor-made PHB-based nanocomposites for high performance applications produced from environmentally friendly production routes
dc.relation.publisherversionhttp://dx.doi.org/10.1016/j.nbt.2013.10.004por
dc.subjectWheat straw hydrolysatespor
dc.subjectPoly 3-hydroxybutyratepor
dc.subjectBurkholderia saccharipor
dc.subjectCarbon catabolite repressionpor
dc.subjectAgricultural lignocellulosic residuespor
dc.subjectHigh cell density culturespor
dc.titleEnhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysatespor
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlePRODUCTION OF POLY 3-HYDROXYBUTYRATE-CO- 4-HYDROXYBUTYRATE P 3HB-CO-4HB FROM LIGNOCELLULOSIC AGRO-INDUSTRIAL WASTES
oaire.awardTitlePRODUÇÃO DE POLIHIDROXIALCANOATOS UTILIZANDO SUBSTRATOS RESIDUAIS
oaire.awardTitleNew tailor-made PHB-based nanocomposites for high performance applications produced from environmentally friendly production routes
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/246449
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F68587%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F26678%2F2006/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/FP7/246449/EU
oaire.citation.endPage113por
oaire.citation.startPage104por
oaire.citation.titleNew Biotechnologypor
oaire.citation.volume31 (1)por
oaire.fundingStreamFP7
oaire.fundingStreamFP7
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspor
rcaap.typearticlepor
relation.isProjectOfPublicationcd42bd59-f71e-413d-9e60-aa768d0c9991
relation.isProjectOfPublication8b03dfca-bdb8-448e-8333-cbd672ab33ee
relation.isProjectOfPublication54143930-0087-45be-ae76-b932ef0f1af1
relation.isProjectOfPublicatione88d1819-1f6d-4b1d-a864-ec2091b17e8a
relation.isProjectOfPublication.latestForDiscoverye88d1819-1f6d-4b1d-a864-ec2091b17e8a

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