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Laser scanning microdissection - Advantages and pitfalls in forensic diagnosis.

dc.contributor.authorCainé, Laura
dc.contributor.authorCosta, Sergio
dc.contributor.authorCorreia-de-Sá, Paulo
dc.contributor.authorPorto, Maria João
dc.date.accessioned2023-02-16T16:43:37Z
dc.date.available2023-02-16T16:43:37Z
dc.date.issued2017
dc.description.abstractLaser microdissection (LMD) is a technology that has been around for more than 40 years. It combines the amplification power of a microscope with the precision cut of objects allowed by the laser technology. The main use of a Laser Microdissection System (LMS) is for clinical purposes, in order to isolate cells or its components of interest. Only in the last decade has LMD been used for forensic purposes, mainly in sexual assault casework for isolating sperm cells from vaginal swabs. Sexual assault cases are among the most difficult scenarios that forensic laboratories have to deal with. Several factors impact the achievement of a result that either implicates or exonerates a suspect. Among these factors, the most important are the quality of the sample, the amounts of DNA present or even their relative proportions in a mixture. Sometimes the interpretation of the mixture is complex, mainly without reference samples from the victim and the suspect. The LMD technology allows the selection of individual cells based on morphology (e.g. sperm cells opposed to epithelial) or on labeling with specific fluorescent dyes for posterior analysis. Mixture analysis with an azoospermic or oligospermic contributor is even more challenging. In the absence of sperm cells, male and female cells are indistinguishable, requiring the use of specific fluorescent dyes. The use of these techniques can also pave the way for detection of enough cells for analysis. Here we try to gather the main options to undertake laser microdissection analysis in forensic casework, highlighting in particular the pros and cons of combining LMD with fluorescence identification of interest specimens to increase accuracy and to speed-up isolation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationCainé, L., Costa, S., & Correia-de-Sá, P. (2017). Laser scanning microdissection - Advantages and pitfalls in forensic diagnosis. In A. Méndez-Vilas (Ed.), Microscopy and imaging science: practical approaches to applied research and education (pp. 202–207). Formatex Research Center.pt_PT
dc.identifier.isbn978-84-942134-9-6
dc.identifier.urihttp://hdl.handle.net/10400.26/43894
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFormatex Research Centerpt_PT
dc.relationCentro de Investigação Farmacológica e Inovação Medicamentosa
dc.subjectLaser microdissectionpt_PT
dc.subjectForensic medicinept_PT
dc.subjectSexual assaultpt_PT
dc.subjectSperm cells isolationpt_PT
dc.titleLaser scanning microdissection - Advantages and pitfalls in forensic diagnosis.pt_PT
dc.typebook part
dspace.entity.typePublication
oaire.awardTitleCentro de Investigação Farmacológica e Inovação Medicamentosa
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FBIM%2F4308%2F2016/PT
oaire.citation.endPage207pt_PT
oaire.citation.startPage202pt_PT
oaire.citation.titleMicroscopy and imaging science: practical approaches to applied research and educationpt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameCainé
person.givenNameLaura
person.identifierAAR-9087-2020
person.identifier.ciencia-id021A-33DD-869D
person.identifier.orcid0000-0002-6542-4256
person.identifier.scopus-author-id2311978
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typebookPartpt_PT
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relation.isAuthorOfPublication.latestForDiscovery1a1e1ffc-1439-48f2-a4c3-359c66db3ad1
relation.isProjectOfPublication46a8502e-2c8b-412c-8e0f-f467d35a96ec
relation.isProjectOfPublication.latestForDiscovery46a8502e-2c8b-412c-8e0f-f467d35a96ec

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