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Metabolite identification of ibuprofen biodegradation by Patulibacter medicamentivorans under aerobic conditions

2018Journal article Restricted access
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dc.contributor.authorSalgado, Ricardo
dc.contributor.authorBrito, Dulce
dc.contributor.authorNoronha, João P.
dc.contributor.authorAlmeida, Bárbara
dc.contributor.authorBronze, Maria R.
dc.contributor.authorOehmen, Adrian
dc.contributor.authorCarvalho, Gilda
dc.contributor.authorCrespo, Maria T. Barreto
dc.date.accessioned2019-04-30T13:50:31Z
dc.date.available2019-04-30T13:50:31Z
dc.date.issued2018
dc.description.abstractIbuprofen(IBU)is anon-steroidalanti-inflammatorydrugthatisbecomingincreasinglyrecognizedas an important micropollutant to be monitored in wastewater treatment plants (WWTP), since it has been detected in effluents at the µg L−1 level. The IBU metabolites from biological degradation are not completely understood and can represent a threat to natural aquatic systems. P. medicamentivorans was previously isolated from WWTP sludge and found to be capable of IBU degradation. The aerobic biodegradation of ibuprofen by this organism was investigated in a batch lab-scale reactor for the identification of the metabolites formed. The metabolites were analysed and putatively identified by HPLC-DAD-MS/MS and GC-MS and biodegradation pathways were proposed. The toxicity and the biodegradability potential of the metabolites were also investigated. The results showed that IBU biotransformation was achieved by hydroxylation followed by the formation of a carboxylic acid in the IBU molecule and by the formation of a catechol, allowing the aromatic ring cleavage. Two biodegradation pathways were proposed: in one, the metabolites generated from the enzymatic action correspond to a less biodegradable chemical structure of the intermediate products (isobutylbenzene and 3-isobutylphenol), with comparatively higher toxicity; in the other mechanism, more oxidable chemical structures were formed with less toxicity and higher biodegradability. This suggests that the biodegradation of IBU by P. medicamentivorans can take place by more than one mechanism regarding the enzymes formed by this Gram-positive bacterium, with subsequent oxidation of the parent compound to overall more soluble and less toxic compounds to fish, daphnia and green algae.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSalgado, R., Brito, D., Noronha, J. P., Almeida, B., Bronze, R. M., Oehmen,A. Carvalho, G. & Crespo, M. T. (2018). Metabolite identification of ibuprofen biodegradation by Patulibacter medicamentivorans under aerobic conditions. Environmental Technology, 27, 1-16.pt_PT
dc.identifier.doi10.1080/09593330.2018.1502362pt_PT
dc.identifier.issn1479-487X
dc.identifier.urihttp://hdl.handle.net/10400.26/28391
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.titleMetabolite identification of ibuprofen biodegradation by Patulibacter medicamentivorans under aerobic conditionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.titleEnvironmental Technologypt_PT
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT

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