Publicação
Histological assessment and gene expression analysis of intra-oral soft tissue graft donor sites
| datacite.subject.fos | Ciências Médicas::Ciências da Saúde | |
| datacite.subject.sdg | 03:Saúde de Qualidade | |
| dc.contributor.author | Stuhr, Sandra | |
| dc.contributor.author | Nör, Felipe | |
| dc.contributor.author | Gayar, Kareem | |
| dc.contributor.author | Couso-Queiruga, Emilio | |
| dc.contributor.author | Chambrone, Leandro | |
| dc.contributor.author | Gamborena, Iñaki | |
| dc.contributor.author | Kumar, Purnima | |
| dc.contributor.author | Avila-Ortiz, Gustavo | |
| dc.contributor.author | Ganesan, Sukirth M. | |
| dc.date.accessioned | 2026-03-02T16:25:32Z | |
| dc.date.available | 2026-03-02T16:25:32Z | |
| dc.date.issued | 2023-10 | |
| dc.description.abstract | Aim: To determine the structural and gene expression features of different intra-oral soft tissue donor sites (i.e., anterior palate, posterior palate, maxillary tuberosity and retromolar pad). Materials and Methods: Standardized mucosal tissue punch biopsies were collected from at least one donor site per subject. Histological processing was performed to determine tissue morphometry and quantify collagen composition. Site-specific gene distribution was mapped using targeted gene expression analysis and validated using real time polymerase chain reaction (qPCR). Results: A total of 50 samples from 37 subjects were harvested. Epithelial thickness did not differ between sites. However, lamina propria was thicker in the maxillary tuberosity (2.55 ± 0.92 mm) and retromolar pad (1.98 ± 0.71 mm) than in the lateral palate. Type I collagen was the predominant structural protein in the lamina propria (75.06%–80.21%). Genes involving collagen maturation and extracellular matrix regulation were highly expressed in the maxillary tuberosity and retromolar pad, while lipogenesis-associated genes were markedly expressed in the lateral palate. The retromolar pad showed the most distinct gene expression profile, and the anterior and posterior palate displayed similar transcription profiles. Conclusions: Tissue samples harvested from the anterior and posterior palate differed morphologically from those from the maxillary tuberosity and retromolar pad. Each intra-oral site showed a unique gene expression profile, which might impact their biological behaviour and outcomes of soft tissue augmentation procedures. | eng |
| dc.identifier.citation | Stuhr, S., Nör, F., Gayar, K., Couso-Queiruga, E., Chambrone, L., Gamborena, I., Kumar, P., Avila-Ortiz, G., & Ganesan, S. M. (2023). Histological assessment and gene expression analysis of intra-oral soft tissue graft donor sites. Journal of Clinical Periodontology, 50(10), 1360–1370. https://doi.org/10.1111/jcpe.13843 | |
| dc.identifier.doi | 10.1111/jcpe.13843 | |
| dc.identifier.issn | 1600-051X | |
| dc.identifier.uri | http://hdl.handle.net/10400.26/61930 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Wiley | |
| dc.relation.hasversion | https://doi.org/10.1111/jcpe.13843 | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | connective tissue | |
| dc.subject | gene expression | |
| dc.subject | histology | |
| dc.subject | oral mucosa | |
| dc.subject | phenotype | |
| dc.title | Histological assessment and gene expression analysis of intra-oral soft tissue graft donor sites | eng |
| dc.type | contribution to journal | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 1370 | |
| oaire.citation.issue | 10 | |
| oaire.citation.startPage | 1360 | |
| oaire.citation.title | Journal of Clinical Periodontology | |
| oaire.citation.volume | 50 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 |