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Publication
New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling
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Advisor(s)
Abstract(s)
The increase in the consumption of antidepressants is a public health problem worldwide,
as these are a class of compounds widely used in the treatment of several illnesses, such as depression
and anxiety. This work aimed to develop and optimize a method for the quantification of a number of
antidepressants and their metabolites (fluoxetine, venlafaxine, O-desmethylvenlafaxine, citalopram,
sertraline, and paroxetine) in 100 μL of oral fluid using the dried saliva spots (DSS) sampling approach
and gas chromatography coupled with tandem mass spectrometry (GC–MS/MS). The method was
validated, presenting linearity within the studied range, with detection and quantification limits
ranging between 10 and 100 ng/mL, and coefficients of determination (R2) of at least 0.99 for all
analytes. Recoveries were between approximately 13 and 46%. The analysis of precision and accuracy
presented acceptable coefficients of variation and relative errors, considering the criteria usually
accepted in the validation of bioanalytical procedures. The method herein described is the first to be
reported using DSS for the extraction of antidepressants, proving to be a sensitive, simple, and fast
alternative to conventional techniques, and capable of being routinely applied in clinical and forensic
toxicology scenarios
Description
Keywords
Antidepressant Biological sample Dried saliva spots GC–MS/M Drug monitorin
Citation
Soares S, Rosado T, Barroso M, Gallardo E. New Method for the Monitoring of Antidepressants in Oral Fluid Using Dried Spot Sampling. Pharmaceuticals. 2021; 14(12):1284
Publisher
MDPI