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In vitro design of a novel lytic bacteriophage cocktail with therapeutic potential against organisms causing diabetic foot infections

2014-08Journal article Open access
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dc.contributor.authorMendes, João J.
dc.contributor.authorLeandro, Clara
dc.contributor.authorMottola, Carla
dc.contributor.authorBarbosa, Raquel
dc.contributor.authorSilva, Filipa A.
dc.contributor.authorOliveira, Manuela
dc.contributor.authorVilela, Cristina L.
dc.contributor.authorMelo-Cristino, José
dc.contributor.authorGórski, Andrzej
dc.contributor.authorPimentel, Madalena
dc.contributor.authorSão-José, Carlos
dc.contributor.authorCavaco-Silva, Patrícia
dc.contributor.authorGarcia, Miguel
dc.date.accessioned2017-05-29T11:53:51Z
dc.date.available2017-05-29T11:53:51Z
dc.date.issued2014-08
dc.description.abstractIn patients with diabetes mellitus, foot infections pose a significant risk. These are complex infections commonly caused by Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii, all of which are potentially susceptible to bacteriophages. Here, we characterized five bacteriophages that we had determined previously to have antimicrobial and wound-healing potential in chronic S. aureus, P. aeruginosa and A. baumannii infections. Morphological and genetic features indicated that the bacteriophages were lytic members of the family Myoviridae or Podoviridae and did not harbour any known bacterial virulence genes. Combinations of the bacteriophages had broad host ranges for the different target bacterial species. The activity of the bacteriophages against planktonic cells revealed effective, early killing at 4 h, followed by bacterial regrowth to pre-treatment levels by 24 h. Using metabolic activity as a measure of cell viability within established biofilms, we found significant cell impairment following bacteriophage exposure. Repeated treatment every 4 h caused a further decrease in cell activity. The greatest effects on both planktonic and biofilm cells occurred at a bacteriophage : bacterium input multiplicity of 10. These studies on both planktonic cells and established biofilms allowed us to better evaluate the effects of a high input multiplicity and a multiple-dose treatment protocol, and the findings support further clinical development of bacteriophage therapy.pt_PT
dc.description.sponsorshipsupported by TechnoPhage S.A. and Tecnifar; Foundation for Science and Technology (PTDC/SAUMIC/122816/2010 – ‘Biofilms in diabetic foot: microbial virulence characterization and cross-talk of major isolates’); FCT PhD fellowship (SFRH/BD/72872/2010); FCT ‘Ciência 2007’ programmept_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMendes J, Leandro C, Mottola C, Barbosa R, Silva F, Oliveira M, Vilela C, Melo-Cristino J, Górski A, Pimentel M, São-José C, Cavaco-Silva P, Garcia M. J. Med. Microbiol. 63(8):1055-1065 doi:10.1099/jmm.0.071753-0pt_PT
dc.identifier.doi10.1099/jmm.0.071753-0pt_PT
dc.identifier.issn0022-2615
dc.identifier.urihttp://hdl.handle.net/10400.26/18417
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMicrobiology Societypt_PT
dc.relationBACTERIAL CROSS-TALK IN BIOFILM-ENCLOSED MIXED POPULATIONS FROM DIABETIC FOOT INFECTION
dc.relation.publisherversionhttp://jmm.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.071753-0pt_PT
dc.subjectAntimicrobial agentspt_PT
dc.subjectchemotherapypt_PT
dc.titleIn vitro design of a novel lytic bacteriophage cocktail with therapeutic potential against organisms causing diabetic foot infectionspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleBACTERIAL CROSS-TALK IN BIOFILM-ENCLOSED MIXED POPULATIONS FROM DIABETIC FOOT INFECTION
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-MIC%2F122816%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F72872%2F2010/PT
oaire.citation.endPage1065pt_PT
oaire.citation.startPage1055pt_PT
oaire.citation.titleJournal of Medical Microbiologypt_PT
oaire.citation.volume63(Pt8)pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication99f1231a-3bb9-4f81-b21c-d97b8a2f3e3e
relation.isProjectOfPublication70a2d91c-040a-4d56-8e2f-a7cd8086cdae
relation.isProjectOfPublication.latestForDiscovery99f1231a-3bb9-4f81-b21c-d97b8a2f3e3e

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