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Avaliação da associação entre lúpus eritematoso sistémico e doença periodontal : uma abordagem bioinformática
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Authors
Abstract(s)
Introdução: A doença periodontal é uma doença inflamatória que afeta os tecidos de sustentação dos dentes e pode sofrer influencia imunológica e pode também estar relacionada com fatores de risco genéticos. O objetivo deste trabalho foi avaliar através da randomização mendeliana e rede de interação proteica a associação de causa-efeito entre o lúpus eritematoso sistémico e a doença periodontal.
Metodologia: Foi utilizada a plataforma MrBase para realização da randomização mendeliana. A pesquisa inicial dos dados populacionais disponíveis foi feita a partir do banco de dados Catálogo do Instituto Nacional de Pesquisa do Genoma Humano – Instituto Europeu de Bioinformática de Estudos da Associação do Genoma Humano, e foi realizada por fenótipo, usando como termos de busca systemic lupus erythematosus para exposição e periodontal disease para desfecho. Para análise da rede de interação proteína-proteína, foi utilizado a ferramenta STRING, onde foram geradas as redes de interação a partir dos dados dos genes envolvidos na doença periodontal e lúpus eritematoso sistémico, identificados na base de dados.
Resultados: A randomização mendeliana encontrou um efeito positivo fraco, porém sem significância estatística na relação entre o Lúpus Eritematoso Sistémico e a doença periodontal, MR Egger (B 0.271 SE= 0.157 p= 0.098. A análise da rede de interação proteína-proteína mostrou haver mais interações que o esperado para o conjunto de genes analisados. Algumas das interações identificados foram a proteína RUNX2 interagindo com PSMB6 e as proteínas IGF2R e PLG apresentando interação com PLAUR, enquanto ANGPT1 teve interação com GRB2 e PLD2.
Conclusão: A randomização mendeliana demonstrou que pode existir uma associação fraca entre o lúpus eritematoso sistémico e a doença periodontal, sem apresentar significância estatística. A análise da rede de interação demostrou haver proteínas de ambas as condições que interagem, interação essa superior ao que seria esperado. Essas proteínas devem ser alvo de mais estudos a fim de ser possível avaliar melhor a relação entre a doença periodontal e o lúpus eritematoso sistémico.
Introduction: Periodontal disease is an inflammatory disease that affects the supporting tissues of teeth and may be immunologically influenced and may also be related to genetic risk factors. The aim of this study was to evaluate, through Mendelian randomization and a protein interaction network, the association, including cause-effect, between systemic lupus erythematosus and periodontal disease. Methods: The MrBase app was used to analyze Mendelian randomization using data available from the Catalog of the National Institute for Human Genome Research – European Institute for Bioinformatics and Studies of the Human Genome Association, which was performed by phenotype using systemic lupus erythematosus as search terms for exposure and periodontal disease for result. For analysis of the protein-protein interaction network, the STRING tool was used, where the interaction networks were generated from the data of genes involved in periodontal disease and allergic disease identified in the database. Results: Mendelian randomization found a weak positive effect, but without statistical significance in the relationship between Systemic Lupus Erythematosus and periodontal disease, MR Egger (B 0.271 SE= 0.157 p= 0.098). The analysis of the protein-protein interaction network showed that there were more interactions than expected for the set of genes analyzed. With RUNX2 protein interacting with PSMB6 and IGF2R and PLG proteins interacting with PLAUR, while ANGPT1 interacted with GRB2 and PLD2. Conclusion: Mendelian randomization demonstrated that there may be a weak association between systemic lupus erythematosus and periodontal disease, without showing statistical significance. The analysis of the interaction network showed that there are proteins from both conditions that interact, which interaction is superior to what would be expected. These proteins should be further studied in order to better assess the relationship between periodontal disease and systemic lupus erythematosus.
Introduction: Periodontal disease is an inflammatory disease that affects the supporting tissues of teeth and may be immunologically influenced and may also be related to genetic risk factors. The aim of this study was to evaluate, through Mendelian randomization and a protein interaction network, the association, including cause-effect, between systemic lupus erythematosus and periodontal disease. Methods: The MrBase app was used to analyze Mendelian randomization using data available from the Catalog of the National Institute for Human Genome Research – European Institute for Bioinformatics and Studies of the Human Genome Association, which was performed by phenotype using systemic lupus erythematosus as search terms for exposure and periodontal disease for result. For analysis of the protein-protein interaction network, the STRING tool was used, where the interaction networks were generated from the data of genes involved in periodontal disease and allergic disease identified in the database. Results: Mendelian randomization found a weak positive effect, but without statistical significance in the relationship between Systemic Lupus Erythematosus and periodontal disease, MR Egger (B 0.271 SE= 0.157 p= 0.098). The analysis of the protein-protein interaction network showed that there were more interactions than expected for the set of genes analyzed. With RUNX2 protein interacting with PSMB6 and IGF2R and PLG proteins interacting with PLAUR, while ANGPT1 interacted with GRB2 and PLD2. Conclusion: Mendelian randomization demonstrated that there may be a weak association between systemic lupus erythematosus and periodontal disease, without showing statistical significance. The analysis of the interaction network showed that there are proteins from both conditions that interact, which interaction is superior to what would be expected. These proteins should be further studied in order to better assess the relationship between periodontal disease and systemic lupus erythematosus.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Doença periodontal Lúpus eritematoso sistémico Randomização mendeliana Rede de interação proteína-proteína