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Comparative study of sample preparation procedures to determine the main compounds in ayahuasca beverages by QuEChERS and high‐performance liquid chromatography analysis

2024Journal article Open access
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dc.contributor.authorGonçalves, Joana
dc.contributor.authorRosado, Tiago
dc.contributor.authorBarroso, Mário
dc.contributor.authorRestolho, José
dc.contributor.authorFernández, Nicolás
dc.contributor.authorLuís, Ângelo
dc.contributor.authorGallardo, Eugenia
dc.contributor.authorDuarte, Ana Paula
dc.date.accessioned2024-05-13T11:03:26Z
dc.date.available2024-05-13T11:03:26Z
dc.date.issued2024
dc.description.abstractIntroduction : Ayahuasca is a psychoactive drink originally consumed by indigenous people of the Amazon. The lack of regulation of this drink leads to uncontrolled consumption, and it is often consumed in religious contexts. Objective :The aim of this work is to compare three miniaturised extraction techniques for extracting the main ayahuasca compounds from beverages. Methodology:Three sample pretreatment techniques were evaluated (dispersive liquid–liquid microextraction [DLLME], microextraction by packed sorbent [MEPS] and QuEChERS [Quick, Easy, Cheap, Effective, Rugged and Safe]) for the simultaneous extraction of N,N-dimethyltryptamine (DMT), tetrahydroharmine (THH), harmine, harmaline, harmol and harmalol from ayahuasca beverage samples. Then, the most promising technique (QuEChERS) was chosen to pre-concentrate the analytes, subsequently detected by high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD). Results: The procedure was optimised, with the final conditions being 500 μL of extractor solvent, 85 mg of primary secondary amine (PSA) and 4 s of vortexing. The analytical method was validated, showing to be linear between 0.16 and 10 μg/mL for β-carbolines and between 0.016 and 1 μg/mL for DMT, with coefficients of determination (R2) between 0.9968 and 0.9993. The limit of detection (LOD) and lower limit of quantification (LLOQ) were 0.16 μg/mL for all compounds, except for DMT (0.016 μg/mL) and extraction efficiencies varied between 60.2% and 88.0%. Conclusion: The analytical methodology proved to be accurate and precise, with good linearity, LODs and LLOQs. This method has been fully validated and successfully applied to ayahuasca beverage samples.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationGonçalves, J., Rosado, T., Barroso, M., Restolho, J., Fernández, N., Luís, Â., Gallardo, E., & Duarte, A. P. (2024). Comparative study of sample preparation procedures to determine the main compounds in ayahuasca beverages by QuEChERS and high-performance liquid chromatography analysis. Phytochemical analysis : PCA, 35(6), 1371–1382pt_PT
dc.identifier.doi10.1002/pca.3370pt_PT
dc.identifier.issn1099-1565
dc.identifier.urihttp://hdl.handle.net/10400.26/50812
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWileypt_PT
dc.relationalterado para: “Avaliação in vitro associados ao consumo de Ayahuasca” AVALIAÇÃO IN VITRO DOS EFEITOS TOXICOLÓGICOS ASSOCIADOS AO CONSUMO DE AYAHUASCA.
dc.relationHealth Sciences Research Centre
dc.relationHealth Sciences Research Centre
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectAyahuascapt_PT
dc.subjectDLLMEpt_PT
dc.subjectMEPSpt_PT
dc.subjectQuEChERSpt_PT
dc.titleComparative study of sample preparation procedures to determine the main compounds in ayahuasca beverages by QuEChERS and high‐performance liquid chromatography analysispt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlealterado para: “Avaliação in vitro associados ao consumo de Ayahuasca” AVALIAÇÃO IN VITRO DOS EFEITOS TOXICOLÓGICOS ASSOCIADOS AO CONSUMO DE AYAHUASCA.
oaire.awardTitleHealth Sciences Research Centre
oaire.awardTitleHealth Sciences Research Centre
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_CENTRO/SFRH%2FBD%2F149360%2F2019/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00709%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00709%2F2020/PT
oaire.citation.titlePhytochemical Analysispt_PT
oaire.fundingStreamPOR_CENTRO
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameBarroso
person.givenNameMário
person.identifier.ciencia-id5110-F476-ECCC
person.identifier.orcid0000-0001-8848-2734
person.identifier.scopus-author-id35329243100
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscovery294b555e-48c4-4013-b981-b8917400c56c
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