A carregar...
Publicação
Qual o timing de ataque ácido numa resina infiltrante de esmalte após remineralização com CPP/ACP?
| Nome: | Descrição: | Tamanho: | Formato: | |
|---|---|---|---|---|
| 13.95 MB | Adobe PDF |
Autores
Orientador(es)
Resumo(s)
Objetivos - Quantificar a microdureza de superfície do esmalte após indução artificial de
cárie, quando se aplica um pré-tratamento com Caseína Fosfopetidea – Fosfato de Cálcio Amorfo, seguido de uma aplicação uma resina infiltrante de esmalte, com distintos tempos de ataque ácido.
Materiais e métodos – 48 espécimes foram obtidos a partir de 12 molares humanos e
aleatoriamente distribuídos em 4 grupos (A-D). Todos os grupos passam por indução
artificial de white spot através de solução tampão de acido láctico durante 6 dias, e
tratamento com CPP-ACP durante 4 semanas. O grupo A não passa por mais nenhum
tratamento, enquanto que os grupos B-D são tratados com resinas infiltrantes com
diferentes timings de condicionamento ácido, de 2, 3 e 4 minutos respetivamente. Após
tratamento foram selecionados, aleatoriamente, 10 espécimes que foram submetidos a
teste de microdureza de superfície através da técnica de Vickers e 2 espécimes foram
visualizados através de microscopia eletrónica de varrimento (MEV). Na análise
estatística foram aplicados os testes ANOVA a um fator e o post-hoc de Games-Howell,
considerando um nível de significância de 5%.
Resultados – Foram demonstradas diferenças na microdureza entre grupos (p<0,05). No
entanto, as médias do grupo A e B não diferem significativamente entre si (p=0,128), nem as dos grupos C e D (p=0,686). Já as médias de B e C diferem estatisticamente (p=0,001).
Segundo a análise a MEV, o grupo A apresenta-se mais homogéneo com menor exposição de lacunas comparativamente com os grupos B, C e D, que se apresentam mais heterogéneo.
Conclusões – O grupo A tratado com CPP-ACP e o grupo B tratado com CPP-ACP e
com 2 minutos de condicionamento ácido apresentam valores semelhantes de microdureza. Os grupos C e D com condicionamento ácido superior a 2 minutos
apresentam microdureza inferior, sugerindo maior desmineralização.
Objectives – To quantify the surface microhardness of the enamel after artificial induction of caries, when a pretreatment with Casein Fosfopetidea - Amorphous Calcium Phosphate is applied, followed by an enamel infiltrating resin, with different times of acid attack. Materials and methods – 48 specimens were obtained from 12 human molars and randomly distributed in 4 groups (A-D). All groups undergo artificial white spot induction through lactic acid buffer solution for 6 days, and CPP-ACP treatment for 4 weeks. Group A does not undergo any further treatment, while the groups B-D were treated with infiltrating resins with different acid conditioning timings, 2, 3 and 4 minutes respectively. After treatment, 10 randomly selected specimens were submitted to a surface microhardness test by Vickers technique and 2 randomly selected specimens were visualized by scanning electron microscopy (SEM). Statistical analysis was performed using the one-way ANOVA test and the post-hoc Games-Howell, Results – Differences in microhardness between groups were demonstrated (p <0.05). However, the means of group A and B did not differ significantly between themselves (p = 0.128), neither those of groups C and D (p = 0.686). The mean values of B and C differ statistically (p = 0.001). According to the SEM analysis, group A is more homogeneous with less exposure of gaps compared to groups B, C and D, which are more heterogeneous. Conclusions – Group A treated with CPP-ACP and group B treated with CPP-ACP and with 2 minutes of acid conditioning presented similar values of microhardness. Groups C and D with acid conditioning for more than 2 minutes have a lower microhardness, suggesting a greater demineralization.
Objectives – To quantify the surface microhardness of the enamel after artificial induction of caries, when a pretreatment with Casein Fosfopetidea - Amorphous Calcium Phosphate is applied, followed by an enamel infiltrating resin, with different times of acid attack. Materials and methods – 48 specimens were obtained from 12 human molars and randomly distributed in 4 groups (A-D). All groups undergo artificial white spot induction through lactic acid buffer solution for 6 days, and CPP-ACP treatment for 4 weeks. Group A does not undergo any further treatment, while the groups B-D were treated with infiltrating resins with different acid conditioning timings, 2, 3 and 4 minutes respectively. After treatment, 10 randomly selected specimens were submitted to a surface microhardness test by Vickers technique and 2 randomly selected specimens were visualized by scanning electron microscopy (SEM). Statistical analysis was performed using the one-way ANOVA test and the post-hoc Games-Howell, Results – Differences in microhardness between groups were demonstrated (p <0.05). However, the means of group A and B did not differ significantly between themselves (p = 0.128), neither those of groups C and D (p = 0.686). The mean values of B and C differ statistically (p = 0.001). According to the SEM analysis, group A is more homogeneous with less exposure of gaps compared to groups B, C and D, which are more heterogeneous. Conclusions – Group A treated with CPP-ACP and group B treated with CPP-ACP and with 2 minutes of acid conditioning presented similar values of microhardness. Groups C and D with acid conditioning for more than 2 minutes have a lower microhardness, suggesting a greater demineralization.
Descrição
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Palavras-chave
Fosfato de cálcio Remineralização White spot Resina infiltrante