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Effect of Urolithin A on bovine sperm capacitation and in vitro fertilization

2023-07-19Master thesis Embargoed access
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dc.contributor.advisorDuarte, Sofia Alexandra Giestas Cancela
dc.contributor.authorJorge, Maria Manuela Martins Silvério Pereira
dc.date.accessioned2024-07-03T14:17:30Z
dc.date.embargo2026-08-16
dc.date.issued2023-07-19
dc.description.abstractReactive oxygen species (ROS) play a critical role in the functional competence of sperm cells. Conversely, excessive generation of ROS can impair sperm function, including their ability for fertilization. Urolithin A (UA), a natural metabolite with anti-aging and antioxidant properties, was investigated for the first time in bovine sperm cells in the present study. Firstly, different doses of UA (0, 1, and 10 µM; 8-16 sessions) were used during the capacitation process of frozen-thawed bovine sperm. Sperm motility was assessed using optical microscopy and CASA. Sperm vitality (eosin-nigrosin), ROS and ATP levels, as well as mitochondrial membrane potential (JC1) and oxy- gen consumption were evaluated. A second experiment to test the effect of different doses of UA (0, 1, and 10 µM; 6 sessions) in both the capacitation medium, as above, and the fertilization medium, was also implemented. The embryonic development and quality were evaluated. UA at a concen- tration of 1 µM significantly improved sperm movement quality (p < 0.025). There was a trend to- wards an increase in the oxygen consumption rate (OCR) of capacitated sperm with 1 µM and 10 µM UA supplementation. Moreover, an increase in ATP levels (p < 0.01) was observed, accompa- nied by a reduction in ROS levels at the higher UA concentration. These results suggest that UA may enhance spermatozoa mitochondrial function. UA almost doubled the number of produced embryos without significant differences in quality. Presented results further support previous find- ings indicating the potential therapeutic value of UA for addressing reproductive sub/infertility problems and improving ART results.pt_PT
dc.identifier.tid203529340pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/51192
dc.language.isoengpt_PT
dc.subjectARTpt_PT
dc.subjectBiotechnologiespt_PT
dc.subjectMitochondriapt_PT
dc.subjectOocytept_PT
dc.subjectReproductivept_PT
dc.subjectROSpt_PT
dc.subjectSpermatozoapt_PT
dc.subjectUrolithin Apt_PT
dc.titleEffect of Urolithin A on bovine sperm capacitation and in vitro fertilizationpt_PT
dc.typemaster thesis
dspace.entity.typePublication
rcaap.rightsembargoedAccesspt_PT
rcaap.typemasterThesispt_PT
thesis.degree.nameMedicina Veterináriapt_PT

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EUVG - Dissertações do Mestrado Integrado em Medicina Veterinária