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Caracterização da composição inorgânica de um particulado de dentina autógena humana mineralizada : estudo piloto in vitro
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Abstract(s)
Objetivos: Analisar a eficácia da solução básica alcoólica na eliminação bacteriana, bem como a composição inorgânica do particulado de dentina autógena humana mineralizada de várias partes do dente (coroa, raiz e dente completo).
Materiais e métodos: Nos laboratórios do Centro de investigação interdisciplinar Egas Moniz, foram triturados dentes pertencentes aos principais grupos dentários (incisivos centrais, laterais, caninos, pré-molares e molares) com recurso ao equipamento “Smart Dentin Grinder®” e obtidos 16 particulados, um dos quais utilizado para avaliar a eficácia da solução básica alcoólica “cleanser”. Os restantes foram divididos em três grupos, A – coroa triturada, B – raiz triturada e C – dente completo triturado, formando-se, por sua vez, cinco subgrupos em cada um deles. A composição elementar das amostras foi obtida através da análise pelo sistema espectrómetro de fluorescência de raios x por dispersão em comprimento de onda (WDXRF). A análise estatística foi realizada com recurso ao software SPSS, de forma a avaliar a existência de diferenças estatísticas significativas relativamente ao rácio cálcio/fósforo de entre os grupos estudados.
Resultados: A eficácia da solução básica alcoólica foi verificada. Existem diferenças estatisticamente significativas no que respeita ao rácio cálcio/fósforo de entre os grupos estudados.
Conclusão: Este trabalho de investigação permitiu caracterizar a composição inorgânica do particulado de dentina autógena humana mineralizada dos principais constituintes dentários (coroa e raiz), bem como da sua totalidade. Verificaram-se diferenças estatisticamente significativas em relação ao rácio cálcio/fósforo. São necessários mais estudos “in vitro”, de forma a caracterizar a composição inorgânica do particulado de dentina autógena humana mineralizada, aumentando consideravelmente o tamanho da amostra.
Objectives: To assess the efficacy of the basic alcoholic solution in bacterial elimination, as well as the inorganic composition of the autogenous human dentin mineralized from various parts of the tooth (crown, root and full tooth) particulate. Materials and methods: In the interdisciplinary research center of investigation Egas Moniz, teeth from the main dental groups (central incisors, lateral incisors, canines, premolars and molars) were grinded using the “Smart Dentin Grinder®” equipment and 16 particulates were obtained, one of which was used to evaluate the efficacy of the basic alcoholic solution “cleanser”. The remaining particulates were divided into three groups, A – grinded crown, B – grinded root and C – grinded full tooth and five subgroups were created inside each one. The elementary composition of the samples was obtained through the wavelength dispersion x-ray fluorescence spectrometer system (WDXRF) analysis. The statistical analysis used the SPSS software and evaluated the significant differences between the calcium/phosphorus ratio in the studied groups. Results: The efficacy of the basic alcoholic solution was verified. There were statistical differences in the calcium/phosphorus ratio in the studied groups. Conclusion: This research paper allowed for the characterization of the inorganic composition of the autogenous human dentin mineralized from the main dental elements (crown and root) as well as the full tooth particulate. Significant statistical differences were found regarding the calcium/ phosphorus ratio. More “in vitro” studies are required to characterize the inorganic composition of mineralized human autogenous dentine particule, greatly increasing the sample size.
Objectives: To assess the efficacy of the basic alcoholic solution in bacterial elimination, as well as the inorganic composition of the autogenous human dentin mineralized from various parts of the tooth (crown, root and full tooth) particulate. Materials and methods: In the interdisciplinary research center of investigation Egas Moniz, teeth from the main dental groups (central incisors, lateral incisors, canines, premolars and molars) were grinded using the “Smart Dentin Grinder®” equipment and 16 particulates were obtained, one of which was used to evaluate the efficacy of the basic alcoholic solution “cleanser”. The remaining particulates were divided into three groups, A – grinded crown, B – grinded root and C – grinded full tooth and five subgroups were created inside each one. The elementary composition of the samples was obtained through the wavelength dispersion x-ray fluorescence spectrometer system (WDXRF) analysis. The statistical analysis used the SPSS software and evaluated the significant differences between the calcium/phosphorus ratio in the studied groups. Results: The efficacy of the basic alcoholic solution was verified. There were statistical differences in the calcium/phosphorus ratio in the studied groups. Conclusion: This research paper allowed for the characterization of the inorganic composition of the autogenous human dentin mineralized from the main dental elements (crown and root) as well as the full tooth particulate. Significant statistical differences were found regarding the calcium/ phosphorus ratio. More “in vitro” studies are required to characterize the inorganic composition of mineralized human autogenous dentine particule, greatly increasing the sample size.
Description
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Keywords
Particulado de dentina autógena Preservação óssea alveolar Particulado dentário autólogo Dentina na engenharia tecidular óssea Smart dentin grinder WDXRF