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Biological Evidences and Sexual Assault in Forensic Medicine

dc.contributor.authorVieira, Claudia
dc.contributor.authorLucas, Isabel
dc.contributor.authorRibeiro, Teresa
dc.contributor.authorCosta Santos, J.
dc.contributor.authorEspinheira, Rosa
dc.date.accessioned2022-07-07T15:44:19Z
dc.date.available2022-07-07T15:44:19Z
dc.date.issued2009-09
dc.descriptionTrabalho apresentado sob a forma de poster no evento: IV Mediterranean Academy of Forensic Sciences Meeting, em Belek, Antalya, Turkyept_PT
dc.description.abstractIntroduction Sexual assault crimes are ubiquitous; usually unwitnessed, so victim’s statements may be the only direct evidence that it has occurred. Medical examination and biological samples collection are of inequivocal importance. Presence of spermatozoa in smears, elevated acid phosphatase and the presence of Y-STR profiler in the victim’s evidence’s, could be crucial to corroborate victim’s testimony. The Y chromosome is far the most important scientific evidence, not only due to it’s gender specificity and easy amplification in a male/female mixtures, but also because it can easily contribute for the number of unrelated male contributor’s estimation in an biological evidence. The aim of this study is to ascertain a few characteristics concerning sexual harassment and a Y-haplotype profile obtention, which may help to prove or disprove a link between individuals and objects or places. Methodology Biological samples from 782 sexual assault cases were studied. Glass slide vaginal and anal smears were stained with Papanicolau’s and Harris Hematoxilin Solution. For the phosphatase test, a commercially available test paper strip was used (Phosphatesmo KM). DNA was extracted by phenol chloroform method and PCR amplification was performed with Powerplex® Y (Promega) or AmpF1STR Y-Filer (Applied Biosystems). Fragment analysis was done by capillary electrophoresis in an ABI 3130xl. Laboratory results were compared with other data such as, victim age, alleged assailant number, time between the act and collection of biological samples. Significant variables were entered in a logistic regression model using SPSS 17.0 for data analysis. Results and conclusion Descriptive analysis reveals that victim’s age varies between 1 to 90 years old, but almost 20% were under 12 years old. Almost 87% of cases had one perpetrator. Only 10% of the victim’s declared that they had consent sexual intercourse, in the previous 48 hours and 32% take a bath before the exam. Another variable studied was the mean time of 15 hours between the act and biological sample collection. Logistic regression demonstrates that the last two variables are of considerable importance in Y-profile obtention. Concerning acid phosphatase results a male genetic identification was obtained in 12,5 % of the negative cases. In conclusion, Y-STRs study, due to their sensitivity and specificity, should be used in every sexual assault cases, contributing to the final identification of perpetrator’s, and corroborating the victim’s story.pt_PT
dc.description.versioninfo:eu-repo/semantics/draftpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/41301
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectSexual assaultpt_PT
dc.subjectY- STRSpt_PT
dc.subjectSemen detetionpt_PT
dc.titleBiological Evidences and Sexual Assault in Forensic Medicinept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceBelek, Antalya, Turkyept_PT
oaire.citation.titleIV Mediterranean Academy of Forensic Sciences Meetingpt_PT
person.familyNameVieira
person.familyNameLucas
person.familyNameRibeiro
person.familyNameSantos
person.givenNameClaudia
person.givenNameIsabel
person.givenNameTeresa
person.givenNameJorge
person.identifier.orcid0000-0002-5897-9122
person.identifier.orcid0000-0001-6339-0008
person.identifier.orcid0000-0002-2903-0006
person.identifier.orcid0000-0002-8925-3068
person.identifier.scopus-author-id24729372100
person.identifier.scopus-author-id7402389332
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublicationcf804b4b-03b6-4959-a247-ba0920c31cdd
relation.isAuthorOfPublication.latestForDiscoverydc5e10df-512e-4d5d-b51d-4b786707877c

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