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Desenvolvimento, otimização e validação de um método para deteção e quantificação de fármacos e pesticidas em amostras de sangue animal
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Orientador(es)
Resumo(s)
Enquadramento: A alteração legislativa no Código Penal foi o grande suporte e alavanca na defensa dos direitos dos animais de companhia.
Objetivo: Desenvolver, otimizar e validar um método para a identificação de fármacos e pesticidas organofosforados em amostras biológicas (sangue) de cães e gatos por cromatografia gasosa acoplada a espectrometria de massa para dar resposta a situações de envenenamento de animais de companhia com validade em tribunal.
Materiais e métodos: A metodologia utilizada é constituída por dois métodos. O método de extração em fase sólida (SPE) e o método confirmatório de cromatografia acoplada à espetrometria de massa (GC/MS).
Resultados: De modo a validar o método pela guideline Standard Practides for Method Validation in Forensic Toxicology foi possível identificar um limite de deteção (LOD) a 50 ng/mL para 8 compostos.
Conclusão: A análise toxicológica em animais de companhia torna-
Background: The legislative change in the Penal Code was the great support and leverage in the defense of the rights of companion animals. Objective: To develop, optimize, and validate a method for the identification of drugs and organophosphate pesticides in biological (blood) of dogs and cats by gas chromatography coupled with mass spectrometry to respond to situations of poisoning of pets with court validity. Materials and methods: The methodology used is obtained by two methods. The solid phase extraction method (SPE) and the confirmation method of chromatography coupled with mass spectrometry (GC/MS). Results: In order to validate the method by the guideline, standard practices for method validation in forensic toxicology it was possible to identify a limit of detection (LOD) of 50 ng/mL for 8 compounds. Conclusion: A toxicological analysis in companion animals becomes a challenging and important process in the diagnosis in cases of poisoning.
Background: The legislative change in the Penal Code was the great support and leverage in the defense of the rights of companion animals. Objective: To develop, optimize, and validate a method for the identification of drugs and organophosphate pesticides in biological (blood) of dogs and cats by gas chromatography coupled with mass spectrometry to respond to situations of poisoning of pets with court validity. Materials and methods: The methodology used is obtained by two methods. The solid phase extraction method (SPE) and the confirmation method of chromatography coupled with mass spectrometry (GC/MS). Results: In order to validate the method by the guideline, standard practices for method validation in forensic toxicology it was possible to identify a limit of detection (LOD) of 50 ng/mL for 8 compounds. Conclusion: A toxicological analysis in companion animals becomes a challenging and important process in the diagnosis in cases of poisoning.
Descrição
Dissertação para obtenção do grau de Mestre no Instituto Universitário Egas Moniz
Palavras-chave
Toxicologia e química analítica SPE-GC/MS Animais de companhia Legislação