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Chlamydia trachomatis: when the virulence-associated genome backbone imports a prevalence-associated major antigen signature

2019-11-07Journal article Open access
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dc.contributor.authorBorges, Vítor
dc.contributor.authorCordeiro, Dora
dc.contributor.authorSalas, Ana Isabel
dc.contributor.authorLodhia, Zohra
dc.contributor.authorCorreia, Cristina
dc.contributor.authorIsidro, Joana
dc.contributor.authorFernandes, Cândida
dc.contributor.authorRodrigues, Ana Maria
dc.contributor.authorAzevedo, Jacinta
dc.contributor.authorAlves, João
dc.contributor.authorRoxo, João
dc.contributor.authorRocha, Miguel
dc.contributor.authorCôrte-Real, Rita
dc.contributor.authorVieira, Luís
dc.contributor.authorBorrego, Maria José
dc.contributor.authorGomes, João Paulo
dc.date.accessioned2024-06-18T11:23:18Z
dc.date.available2024-06-18T11:23:18Z
dc.date.issued2019-11-07
dc.description.abstractChlamydia trachomatis is the most prevalent sexually transmitted bacterium worldwide and the causative agent of trachoma. Its strains are classified according to their ompA genotypes, which are strongly linked to differential tissue tropism and disease outcomes [ocular disease, urogenital disease and lymphogranuloma venereum (LGV)]. While the genome-based species phylogenetic tree presents four main clades correlating with tropism/prevalence, namely ocular, LGV, urogenital T1 (more prevalent genotypes) and urogenital T2 (less prevalent genotypes), inter-clade exchange of ompA is considered a rare phenomenon probably mediating marked tropism alterations. An LGV epidemic, associated with the clonal expansion of the L2b genotype, has emerged in the last few decades, raising concerns particularly due to its atypical clinical presentation (ulcerative proctitis) and circulation among men who have sex with men (MSM). Here, we report an LGV outbreak, mostly affecting human immunodeficiency virus-positive MSM engaging in high-risk sexual practices, caused by an L2b strain with a rather unique non-LGV ompA signature that precluded the laboratory notification of this outbreak as LGV. C. trachomatis whole-genome capture and sequencing directly from clinical samples was applied to deeply characterize the genomic backbone of this novel LGV outbreak-causing clone. It revealed a chimeric genome structure due to the genetic transfer of ompA and four neighbouring genes from a serovar D/Da strain, likely possessing the genomic backbone associated with the more prevalent urogenital genotypes (T1 clade), to an LGV (L2b) strain. The hybrid L2b/D-Da strain presents the adhesin and immunodominant antigen MOMP (major outer membrane protein) (encoded by ompA) with an epitope repertoire typical of non-invasive genital strains, while keeping the genome-dispersed virulence fingerprint of a classical LGV strain. As previously reported for inter-clade ompA exchange among non-LGV clades, this novel C. trachomatis genomic mosaic involving a contemporary epidemiologically and clinically relevant LGV strain may have implications on its transmission, tissue tropism and pathogenic capabilities. The emergence of variants with epidemic and pathogenic potential highlights the need for more focused surveillance strategies to capture C. trachomatis evolution in action.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1099/mgen.0.000313pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.26/51063
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectChlamydia trachomatispt_PT
dc.subjectSexually transmitted infectionspt_PT
dc.subjectLymphogranuloma venereumpt_PT
dc.subjectMen who have sex with menpt_PT
dc.titleChlamydia trachomatis: when the virulence-associated genome backbone imports a prevalence-associated major antigen signaturept_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue11pt_PT
oaire.citation.titleMicrobial Genomicspt_PT
oaire.citation.volume5pt_PT
person.familyNameRoxo
person.familyNameRocha
person.givenNameJoão
person.givenNameMiguel
person.identifier999820
person.identifier.ciencia-id5F19-66C4-5F80
person.identifier.orcid0000-0002-0743-2397
person.identifier.orcid0000-0003-1057-2419
person.identifier.ridAAL-7984-2020
person.identifier.scopus-author-id57204739884
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublication3219f60d-2c30-4396-9e66-67b0dbecae00
relation.isAuthorOfPublicationf47c1e01-1192-4946-89a8-66594bb7364e
relation.isAuthorOfPublication.latestForDiscoveryf47c1e01-1192-4946-89a8-66594bb7364e

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